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anti-CALB1 antibody :: Rabbit Calbindin Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS176994
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
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 Product Name   

Calbindin (CALB1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Calbindin Antibody

 Product Synonym Names    Calbindin; calbindin 1, 28kDa; avian-type antibody; CAB27 antibody; CALB 1 antibody; CALB antibody; CALB1 antibody; CALB1_HUMAN antibody; Calbindin 1 28kDa antibody; Calbindin antibody; Calbindin D28 antibody; D 28K antibody; D-28K antibody; D28K antibody; OTTHUMP00000166027 antibody; OTTHUMP00000225441 antibody; RTVL H protein antibody; Vitamin D dependent calcium binding protein antibody; Vitamin D dependent calcium binding protein avian type antibody; Vitamin D dependent calcium binding protein avian-type antibody; Vitamin D-dependent calcium-binding protein antibody
 Product Gene Name   

anti-CALB1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    114050
 3D Structure    ModBase 3D Structure for P05937
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    No cross reactivity with other proteins.
 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized
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 Immunogen    E Coli-derived human Calbindin recombinant protein (Position: A2-E175). Human Calbindin shares 99% amino acid (aa) sequence identity with mouse and rat Calbindin.
 Contents    Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
 Reconstitution    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-CALB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CALB1 antibody

   Description: Rabbit IgG polyclonal antibody for Calbindin(CALB1) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: Calbindin is a calcium-binding protein belonging to the troponin C superfamily. Calretinin is expressed in central and peripheral nervous system and in many normal and pathological tissues. The rat and human calretinin exhibit 98% sequence homology and 91% homology to many other species. Two calcium binding proteins, calbindin and calretinin, have been reported to be expressed in abundance in Purkinje cells and other cell types in the cerebellum.
 Applications Tested/Suitable for anti-CALB1 antibody   

Western Blot (WB), Immunohistochemistry (IHC) Paraffin

 Application Notes for anti-CALB1 antibody    Western Blot:
Concentration: 0.1-0.5ug/ml
Tested Species: Rat
Predicted Species: Hu
Antigen Retrieval: -

Immunohistochemistry (Paraffin-embedded Section) Concentration: 0.5-1 ug/ml
Tested Species: Hu, Ms, Rat
Predicted Species: -
Antigen Retrieval: By Heat

WB: The detection limit for Calbindin is approximately 0.25ng/lane under reducing conditions.
Tested Species: in-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
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 Immunohistochemistry (IHC) of anti-CALB1 antibody    Figure 1. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CALB1 antibody    Figure 2. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CALB1 antibody    Figure 3. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Mouse Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CALB1 antibody    Figure 4. IHC analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Calbindin was detected in paraffin-embedded section of Rat Kidney Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-Calbindin Antibody (MBS176994) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
anti-CALB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-CALB1 antibody    Figure 5. Western blot analysis of Calbindin using anti-Calbindin antibody (MBS176994).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Kidney Tissue Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calbindin antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Calbindin at approximately 37KD. The expected band size for Calbindin is at 30KD.
anti-CALB1 antibody Western Blot (WB) (WB) image
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 Flow Cytometry (FC/FACS) of anti-CALB1 antibody    Figure 6. Flow Cytometry analysis of SiHa cells using anti-Calbindin antibody (MBS176994).
Overlay histogram showing SiHa cells stained with MBS176994 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (MBS176994,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
anti-CALB1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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 Flow Cytometry (FC/FACS) of anti-CALB1 antibody    Figure 7. Flow Cytometry analysis of Hela cells using anti-Calbindin antibody (MBS176994).
Overlay histogram showing Hela cells stained with MBS176994 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Calbindin Antibody (MBS176994,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
anti-CALB1 antibody Flow Cytometry (FC/FACS) (FC/FACS) image
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NCBI/Uniprot data below describe general gene information for CALB1. It may not necessarily be applicable to this product.
 NCBI GI #    115394
 NCBI GeneID    793
 NCBI Accession #    P05937.2 [Other Products]
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 UniProt Primary Accession #    P05937 [Other Products]
 UniProt Secondary Accession #    B2R696; B7Z9J4 [Other Products]
 UniProt Related Accession #    P05937 [Other Products]
 Molecular Weight    23,609 Da
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 NCBI Official Full Name    Calbindin
 NCBI Official Synonym Full Names    calbindin 1, 28kDa
 NCBI Official Symbol    CALB1 [Similar Products]
 NCBI Official Synonym Symbols   
CALB
[Similar Products]
 NCBI Protein Information    calbindin; D-28K; calbindin D28; RTVL-H protein; calbindin 1, (28kD); vitamin D-dependent calcium-binding protein, avian-type
 UniProt Protein Name    Calbindin
 UniProt Synonym Protein Names   
Calbindin D28; D-28K; Vitamin D-dependent calcium-binding protein, avian-type
 Protein Family    Calbindin
 UniProt Gene Name    CALB1 [Similar Products]
 UniProt Synonym Gene Names    CAB27 [Similar Products]
 UniProt Entry Name    CALB1_HUMAN
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 NCBI Summary for CALB1    Calbindin is a calcium-binding protein belonging to the troponin C superfamily. It was originally described as a 27-kD protein induced by vitamin D in the duodenum of the chick. In the brain, its synthesis is independent of vitamin-D-derived hormones. Calbindin contains 4 active calcium-binding domains, and 2 modified domains that presumably have lost their calcium-binding capacity. The neurons in brains of patients with Huntington disease are calbindin-depleted. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CALB1    CALB1: Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase. Interacts with RANBP9. Belongs to the calbindin family.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 8q21.3

Cellular Component: cell soma; axon; dendrite; synapse; nucleus; cytosol

Molecular Function: vitamin D binding; protein binding; zinc ion binding; calcium ion binding

Biological Process: cytosolic calcium ion homeostasis; long-term memory; short-term memory; regulation of synaptic plasticity; locomotory behavior
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 Research Articles on CALB1    1. The calbindin-D 28k was decreased in the putamen, the dorsal tier of the substantia nigra along the lower border of the red nucleus, and in the cerebellar cortex in multiple system atrophy.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CALB1 antibodyPathways associated with anti-CALB1 antibody
 Reference Product  PubMed Publications
 CA2 antibody  >107 publications with CALB1 and CA2
 S100G antibody  >42 publications with CALB1 and S100G
 TRPV5 antibody  >21 publications with CALB1 and TRPV5
 TRPV6 antibody  >11 publications with CALB1 and TRPV6
 BDNF antibody  >8 publications with CALB1 and BDNF
 GDNF antibody  >4 publications with CALB1 and GDNF
 PVALB antibody  >1 publications with CALB1 and PVALB
 Products by Pathway  Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
 Endocrine And Other Factor-regulated Calcium Reabsorption Pathway antibodies  Endocrine And Other Factor-regulated Calcium Reabsorption Pathway Diagram
Diseases associated with anti-CALB1 antibodyOrgans/Tissues associated with anti-CALB1 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >115 publications with CALB1 and Nervous System Diseases
 Brain Diseases Antibodies  >94 publications with CALB1 and Brain Diseases
 Nerve Degeneration Antibodies  >47 publications with CALB1 and Nerve Degeneration
 Epilepsy Antibodies  >27 publications with CALB1 and Epilepsy
 Disease Models, Animal Antibodies  >24 publications with CALB1 and Disease Models, Animal
 Seizures Antibodies  >18 publications with CALB1 and Seizures
 Kidney Diseases Antibodies  >15 publications with CALB1 and Kidney Diseases
 Neurotoxicity Syndromes Antibodies  >9 publications with CALB1 and Neurotoxicity Syndromes
 Necrosis Antibodies  >9 publications with CALB1 and Necrosis
 Cognition Disorders Antibodies  >8 publications with CALB1 and Cognition Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >461 publications with CALB1 and Brain
 Kidney Antibodies  >99 publications with CALB1 and Kidney
 Blood Antibodies  >52 publications with CALB1 and Blood
 Embryonic Tissue Antibodies  >35 publications with CALB1 and Embryonic Tissue
 Muscle Antibodies  >23 publications with CALB1 and Muscle
 Eye Antibodies  >17 publications with CALB1 and Eye
 Lung Antibodies  >14 publications with CALB1 and Lung
 Connective Tissue Antibodies  >6 publications with CALB1 and Connective Tissue
 Stomach Antibodies  >5 publications with CALB1 and Stomach
 Ear Antibodies  >4 publications with CALB1 and Ear
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