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anti-CASP3 antibody :: Rabbit anti-Mouse Caspase 3 (CASP3) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2003999 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $105 +1 FREE 8GB USB
0.02 mg  /  $125 +1 FREE 8GB USB
0.05 mg  /  $170 +1 FREE 8GB USB
0.1 mg  /  $210 +1 FREE 8GB USB
0.2 mg  /  $315 +1 FREE 8GB USB
1 mg  /  $745 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Caspase 3 (CASP3), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to Caspase 3 (CASP3)

 Product Gene Name   

anti-CASP3 antibody

[Similar Products]
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
Biotin Conjugated Antibody: Caspase 3 (CASP3) (MBS2005976)
 Matching Pairs    Antibody: Caspase 3 (CASP3) (MBS2003999)
Antigen: Caspase 3 (CASP3) (MBS2009683)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
FITC Conjugated Antibody: Caspase 3 (CASP3) (MBS2013972)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
APC-CY7 Conjugated Antibody: Caspase 3 (CASP3) (MBS2045164)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
PE Conjugated Antibody: Caspase 3 (CASP3) (MBS2045165)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
APC-CY7 Conjugated Antibody: Caspase 3 (CASP3) (MBS2045166)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
Cy3 Conjugated Antibody: Caspase 3 (CASP3) (MBS2045167)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
HRP Conjugated Antibody: Caspase 3 (CASP3) (MBS2045168)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
 Matching Pairs    Unconjugated Antibody: Caspase 3 (CASP3) (MBS2003999)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SG IYLDSSYKMD YPEMGICIII NNKNFHKSTG MSSRSGTDVD AANLRETFMG LKYQVRNKND LTREDILELM DSVSKEDHSK RSSFVCVILS HGDEGVIYGT NGPVELKKLT SFFRGDYCRS LTGKPKLFII QACRGTELDC GIETD
 3D Structure    ModBase 3D Structure for P70677
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse
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 Specificity    The antibody is a rabbit polyclonal antibody raised against CASP3. It has been selected for its ability to recognize CASP3 in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Recombinant CASP3 (Ser29~Asp175) expressed in E.coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2045166
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-CASP3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CASP3 antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

 Application Notes for anti-CASP3 antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) of anti-CASP3 antibody    (Figure. Western Blot; Sample. Lane1: Mouse Spleen Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Blood Cells.)
anti-CASP3 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-CASP3 antibody    (Figure. Western Blot; Sample: Recombinant CASP3, Mouse.)
anti-CASP3 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-CASP3 antibody    (Used in DAB staining on formalin fixed paraffin-embedded Liver tissue)
anti-CASP3 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CASP3. It may not necessarily be applicable to this product.
 NCBI GI #    549806822
 NCBI GeneID    12367
 NCBI Accession #    NP_001271338.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001284409.1 [Other Products]
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 UniProt Primary Accession #    P70677 [Other Products]
 UniProt Secondary Accession #    O08668; Q8CHV5; Q9QWI4 [Other Products]
 UniProt Related Accession #    P70677 [Other Products]
 Molecular Weight    31,475 Da
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 NCBI Official Full Name    caspase-3
 NCBI Official Synonym Full Names    caspase 3
 NCBI Official Symbol    Casp3 [Similar Products]
 NCBI Official Synonym Symbols   
CC3; AC-3; Lice; Yama; mldy; CPP32; SCA-1; CASP-3; CPP-32; Caspase-3; A830040C14Rik
[Similar Products]
 NCBI Protein Information    caspase-3
 UniProt Protein Name    Caspase-3
 UniProt Synonym Protein Names   
Apopain; Cysteine protease CPP32; CPP-32
 Protein Family    Caspase
 UniProt Gene Name    Casp3 [Similar Products]
 UniProt Synonym Gene Names    Cpp32; CASP-3; CPP-32; SCA-1 [Similar Products]
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 NCBI Summary for CASP3    This gene encodes a protein that belongs to a highly conserved family of cysteinyl aspartate-specific proteases that function as essential regulators of programmed cell death through apoptosis. Members of this family contain an N-terminal pro-domain and require cleavage at specific aspartate residues to become mature. The protein encoded by this gene belongs to a subgroup of cysteinyl aspartate-specific proteases that are activated by initiator caspases and that perform the proteolytic cleavage of apoptotic target proteins. Mice defective for this gene exhibit a variety of phenotypes including reduced neuronal apoptosis resulting in hyperplasias, hearing loss, attenuated osteogenic differentiation of bone marrow stromal stem cells, and pre- and post-natal lethality. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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 UniProt Comments for CASP3    CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family.

Protein type: Apoptosis; EC 3.4.22.56; Motility/polarity/chemotaxis; Protease

Chromosomal Location of Human Ortholog: 8 B1.1|8 26.39 cM

Cellular Component: cytoplasm; cytosol; membrane raft; nucleus

Molecular Function: aspartic-type endopeptidase activity; cyclin-dependent protein serine/threonine kinase inhibitor activity; cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; hydrolase activity; peptidase activity; phospholipase A2 activator activity; protease binding; protein binding; protein complex binding

Biological Process: apoptosis; B cell homeostasis; cell fate commitment; cellular response to DNA damage stimulus; cellular response to organic substance; erythrocyte differentiation; heart development; intrinsic apoptotic signaling pathway in response to osmotic stress; keratinocyte differentiation; learning and/or memory; negative regulation of activated T cell proliferation; negative regulation of apoptosis; negative regulation of B cell proliferation; negative regulation of cell cycle; negative regulation of cyclin-dependent protein serine/threonine kinase activity; nerve growth factor receptor signaling pathway; neuron apoptosis; neuron differentiation; positive regulation of apoptosis; positive regulation of neuron apoptotic process; protein processing; proteolysis; response to glucose stimulus; response to hydrogen peroxide; response to organic substance; response to UV; response to wounding; sensory perception of sound; T cell homeostasis
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 Research Articles on CASP3    1. findings suggest that caspase-3 activation can trigger necrosis by cleaving GSDME and offer new insights into cancer chemotherapy
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CASP3 antibodyPathways associated with anti-CASP3 antibody
 Reference Product  PubMed Publications
 Casp9 antibody  >7299 publications with CASP3 and Casp9
 Casp8 antibody  >5152 publications with CASP3 and Casp8
 Bcl2 antibody  >1181 publications with CASP3 and Bcl2
 Xiap antibody  >978 publications with CASP3 and Xiap
 Apaf1 antibody  >348 publications with CASP3 and Apaf1
 Birc3 antibody  >47 publications with CASP3 and Birc3
 Birc2 antibody  >41 publications with CASP3 and Birc2
 Dffb antibody  >39 publications with CASP3 and Dffb
 Mapk14 antibody  >23 publications with CASP3 and Mapk14
 Dffa antibody  >5 publications with CASP3 and Dffa
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 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
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Diseases associated with anti-CASP3 antibodyOrgans/Tissues associated with anti-CASP3 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >6413 publications with CASP3 and Necrosis
 Nervous System Diseases Antibodies  >5034 publications with CASP3 and Nervous System Diseases
 Inflammation Antibodies  >3501 publications with CASP3 and Inflammation
 Reperfusion Injury Antibodies  >2247 publications with CASP3 and Reperfusion Injury
 Liver Diseases Antibodies  >2195 publications with CASP3 and Liver Diseases
 Brain Ischemia Antibodies  >1601 publications with CASP3 and Brain Ischemia
 Heart Diseases Antibodies  >1502 publications with CASP3 and Heart Diseases
 Breast Neoplasms Antibodies  >1401 publications with CASP3 and Breast Neoplasms
 Neoplasms, Experimental Antibodies  >1259 publications with CASP3 and Neoplasms, Experimental
 Kidney Diseases Antibodies  >853 publications with CASP3 and Kidney Diseases
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >7401 publications with CASP3 and Brain
 Lung Antibodies  >3095 publications with CASP3 and Lung
 Kidney Antibodies  >1978 publications with CASP3 and Kidney
 Prostate Antibodies  >1199 publications with CASP3 and Prostate
 Eye Antibodies  >1197 publications with CASP3 and Eye
 Skin Antibodies  >1017 publications with CASP3 and Skin
 Intestine Antibodies  >877 publications with CASP3 and Intestine
 Embryonic Tissue Antibodies  >773 publications with CASP3 and Embryonic Tissue
 Pancreas Antibodies  >737 publications with CASP3 and Pancreas
 Spinal Cord Antibodies  >662 publications with CASP3 and Spinal Cord
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