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anti-CASP8 antibody :: Rabbit anti-Human Caspase 8 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2032312 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $100 +1 FREE 8GB USB
0.02 mg  /  $110 +1 FREE 8GB USB
0.05 mg  /  $150 +1 FREE 8GB USB
0.1 mg  /  $195 +1 FREE 8GB USB
0.2 mg  /  $270 +1 FREE 8GB USB
1 mg  /  $645 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Caspase 8 (CASP8), Polyclonal Antibody

★Popular Item★
 Also Known As   

Caspase 8 (CASP8) Polyclonal Antibody

 Product Synonym Names    CAP4; FLICE; MACH; MCH5; Cysteinyl Aspartate Specific Proteinases 8; Apoptotic cysteine protease; FADD-homologous ICE/ced-3-like protease; MORT1-associated ced-3 homolog
 Product Gene Name   

anti-CASP8 antibody

[Similar Products]
 Matching Pairs    Antibody: Caspase 8 (MBS2032312)
Antigen: Caspase 8 (MBS2031598)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
APC-CY7 Conjugated Antibody: Caspase 8 (CASP8) (MBS2048666)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
PE Conjugated Antibody: Caspase 8 (CASP8) (MBS2048669)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
APC-CY7 Conjugated Antibody: Caspase 8 (CASP8) (MBS2048672)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
Cy3 Conjugated Antibody: Caspase 8 (CASP8) (MBS2048675)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
FITC Conjugated Antibody: Caspase 8 (CASP8) (MBS2048677)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
HRP Conjugated Antibody: Caspase 8 (CASP8) (MBS2048680)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
APC-CY7 Conjugated Secondary Antibody: Immunoglobulin G (MBS2090675)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
Unconjugated Secondary Antibody: Immunoglobulin G (MBS2090678)
 Matching Pairs    Unconjugated Antibody: Caspase 8 (MBS2032312)
Biotin Conjugated Antibody: Caspase 8 (CASP8) (MBS2092595)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X98172 mRNA
 3D Structure    ModBase 3D Structure for Q14790
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody is a rabbit polyclonal antibody raised against CASP8. It has been selected for its ability to recognize CASP8 in immunohistochemical staining and western blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4 containing 0.02% NaN3, 50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunoglobulin Type    IgG
 Conjugate    No Conjugate
 Organism Species    Human
 Fragment    CASP8 (Leu7~Asp216)
 Quality Control    Content: The quality control contains recombinant CASP8 (Leu7~Asp216) disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2048672
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-CASP8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-CASP8 antibody    Knock-Out Validation Antibody
 Applications Tested/Suitable for anti-CASP8 antibody   

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)

 Application Notes for anti-CASP8 antibody    Western Blot: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) Knockout Verification of anti-CASP8 antibody    Knockout Verification: Lane 1: Wild-type Hela cell lysate
Lane 2: CASP8 knockout Hela cell lysate
Predicted MW: 26-32,46,54-62kDa ;Observed MW: 60kDa;Primary Ab: 3ug/ml Rabbit Anti-Human CASP8 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-CASP8 antibody Western Blot (WB) Knockout Verification (WB) image
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 Western Blot (WB) of anti-CASP8 antibody    Western Blot; Sample: Lane1: Human Liver Tissue; Lane2: Human HepG2 Cells; Lane3: Human Hela Cells.
anti-CASP8 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-CASP8 antibody    Western Blot; Sample: Recombinant CASP8, Human.
anti-CASP8 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-CASP8 antibody    FITC staining on IHC-P Simple: Hela cells)
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CASP8 antibody    DAB staining on IHC-P; Samples: Human Pancreas Cancer Tissue
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CASP8 antibody    DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CASP8 antibody    DAB staining on IHC-P; Samples: Human Liver Cancer Tissue
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-CASP8 antibody    DAB staining on IHC-P; Samples: Human Breast Cancer Tissue.
anti-CASP8 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for CASP8. It may not necessarily be applicable to this product.
 NCBI GI #    122056474
 NCBI GeneID    841
 NCBI Accession #    NP_001073593.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001080124.1 [Other Products]
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 UniProt Primary Accession #    Q14790 [Other Products]
 UniProt Secondary Accession #    O14676; Q14791; Q14792; Q14793; Q14794; Q14795; Q14796; Q15780; Q15806; Q53TT5; Q8TDI1 [Other Products]
 UniProt Related Accession #    Q14790 [Other Products]
 Molecular Weight    61,836 Da
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 NCBI Official Full Name    caspase-8 isoform C
 NCBI Official Synonym Full Names    caspase 8
 NCBI Official Symbol    CASP8 [Similar Products]
 NCBI Official Synonym Symbols   
CAP4; MACH; MCH5; FLICE; ALPS2B; Casp-8
[Similar Products]
 NCBI Protein Information    caspase-8
 UniProt Protein Name    Caspase-8
 UniProt Synonym Protein Names   
Apoptotic cysteine protease; Apoptotic protease Mch-5; CAP4; FADD-homologous ICE/ced-3-like protease; FADD-like ICE; FLICE
 Protein Family    Caspase
 UniProt Gene Name    CASP8 [Similar Products]
 UniProt Synonym Gene Names    MCH5; CASP-8; FLICE; MACH [Similar Products]
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 NCBI Summary for CASP8    This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This protein is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of this protein suggests that it may interact with Fas-interacting protein FADD. This protein was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Many alternatively spliced transcript variants encoding different isoforms have been described, although not all variants have had their full-length sequences determined. [provided by RefSeq, Jul 2008]
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 UniProt Comments for CASP8    CASP8: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death- inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Interacts with human cytomegalovirus/HHV-5 protein vICA/UL36; this interaction inhibits CASP8 activation. Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. Belongs to the peptidase C14A family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.4.22.61; Protease

Chromosomal Location of Human Ortholog: 2q33.1

Cellular Component: CD95 death-inducing signaling complex; cell body; cytoskeleton; cytosol; membrane raft; mitochondrial outer membrane; mitochondrion; neuron projection; nucleoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity; death receptor binding; identical protein binding; peptidase activity; protein binding; protein complex binding; tumor necrosis factor receptor binding; ubiquitin protein ligase binding

Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; activation of cysteine-type endopeptidase activity involved in apoptotic signaling pathway; apoptosis; B cell activation; cell surface receptor signaling pathway; cellular response to organic cyclic compound; death-inducing signaling complex assembly; macrophage differentiation; natural killer cell activation; negative regulation of extrinsic apoptotic signaling pathway via death domain receptors; negative regulation of I-kappaB kinase/NF-kappaB signaling; nucleotide-binding oligomerization domain containing signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of macrophage differentiation; positive regulation of neuron death; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of proteolysis; proteolysis; proteolysis involved in cellular protein catabolic process; regulation of extrinsic apoptotic signaling pathway via death domain receptors; regulation of tumor necrosis factor-mediated signaling pathway; response to antibiotic; response to cobalt ion; response to cold; response to estradiol; response to ethanol; response to lipopolysaccharide; response to tumor necrosis factor; T cell activation; toll-like receptor 3 signaling pathway; TRAIL-activated apoptotic signaling pathway

Disease: Breast Cancer; Caspase 8 Deficiency; Hepatocellular Carcinoma; Lung Cancer
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 Research Articles on CASP8    1. study shows genetic association of rare variants in CASP8 with Alzheimer's disease and proposes a mechanism of action mediated by decreased enzyme activity; for two CASP8 variants, p.K148R and p.I298V, the association remained significant in a large combined sample
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CASP8 antibodyPathways associated with anti-CASP8 antibody
 Reference Product  PubMed Publications
 CASP3 antibody  >3914 publications with CASP8 and CASP3
 FAS antibody  >2703 publications with CASP8 and FAS
 FADD antibody  >1926 publications with CASP8 and FADD
 BID antibody  >1096 publications with CASP8 and BID
 CFLAR antibody  >796 publications with CASP8 and CFLAR
 TNFRSF10B antibody  >731 publications with CASP8 and TNFRSF10B
 BCL2L1 antibody  >465 publications with CASP8 and BCL2L1
 CASP10 antibody  >226 publications with CASP8 and CASP10
 TNFRSF10A antibody  >178 publications with CASP8 and TNFRSF10A
 CASP6 antibody  >174 publications with CASP8 and CASP6
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway antibodies  Activation, Myristolyation Of BID And Translocation To Mitochondria Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimer's Disease Pathway antibodies  Alzheimer's Disease Pathway Diagram
 Alzheimers Disease Pathway antibodies  Alzheimers Disease Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
Diseases associated with anti-CASP8 antibodyOrgans/Tissues associated with anti-CASP8 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >3275 publications with CASP8 and Neoplasms
 Necrosis Antibodies  >2434 publications with CASP8 and Necrosis
 Drug Toxicity Antibodies  >614 publications with CASP8 and Drug Toxicity
 Adenocarcinoma Antibodies  >488 publications with CASP8 and Adenocarcinoma
 Nervous System Diseases Antibodies  >425 publications with CASP8 and Nervous System Diseases
 Inflammation Antibodies  >419 publications with CASP8 and Inflammation
 Breast Neoplasms Antibodies  >365 publications with CASP8 and Breast Neoplasms
 Liver Diseases Antibodies  >354 publications with CASP8 and Liver Diseases
 Colorectal Neoplasms Antibodies  >294 publications with CASP8 and Colorectal Neoplasms
 Lung Neoplasms Antibodies  >264 publications with CASP8 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1392 publications with CASP8 and Blood
 Liver Antibodies  >595 publications with CASP8 and Liver
 Brain Antibodies  >493 publications with CASP8 and Brain
 Lung Antibodies  >484 publications with CASP8 and Lung
 Prostate Antibodies  >293 publications with CASP8 and Prostate
 Vascular Antibodies  >287 publications with CASP8 and Vascular
 Muscle Antibodies  >283 publications with CASP8 and Muscle
 Bone Antibodies  >255 publications with CASP8 and Bone
 Kidney Antibodies  >247 publications with CASP8 and Kidney
 Heart Antibodies  >222 publications with CASP8 and Heart
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