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anti-CHAT antibody :: Goat ChAT (Choline Acetyltransferase) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502147
Testing Data
Unit / Price
0.1 mL  /  $360 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ChAT (Choline Acetyltransferase), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-Choline Acetyltransferase

 Product Gene Name   

anti-CHAT antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 10; NC_000010.11 (49609095..49665104). Location: 10q11.2
 3D Structure    ModBase 3D Structure for P28329
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Avian, Chicken, Guinea Pig, Human, Mouse, non-Human primates
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 Specificity    Specific for the ~ 70k choline acetyltransferase protein
 Purity/Purification    Affinity Purified (Prepared from goat serum by affinity purification using a column to which the protein immunogen was coupled.)
 Form/Format    100 ul in PBS + 5 mg/ml BSA + 0.2% NaN3.. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Native choline acetyltransferase purified from human placenta
 Immunogen Information    Native choline acetyltransferase purifed from human placenta
 Immunogen Species    Human
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots with chicken, guinea pig, human, mouse, non-human primate and rat tissues.
 Biological Significance    Choline acetyltransferase is a neuronal enzyme which catalyzes the reaction between Acetyl CoA and choline resulting in the formation of acetylcholine. It is therefore found primarily in cholinergic neurons making it a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease (AD) and Down syndrome (Holt et al. 1999). Decreased choline acetyltransferase activity in particular has been shown in Schizophrenic subjects (Karson et al 1993). It has furthermore been demonstrated that in patients with AD, there are significantly lower levels of cortical ChAT that correlate with severity of the disease as measured by loss of neuropsychological function (Baskin et al. 1999).
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 Preparation and Storage    For long term storage -20 degree C is recommended. Stable for at least 1 year.
 Other Notes    Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-CHAT antibody

   Affinity purified goat polyclonal antibody
 Applications Tested/Suitable for anti-CHAT antibody   

Western Blot (WB)

 Application Notes for anti-CHAT antibody    Quality Control: Western blots performed on each lot.
WB: 1:100
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 Testing Data of anti-CHAT antibody    anti-CHAT antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.
 NCBI GeneID    1103
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 UniProt Primary Accession #    P28329 [Other Products]
 UniProt Related Accession #    P28329 [Other Products]
 Molecular Weight    70
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 NCBI Official Synonym Full Names    choline O-acetyltransferase
 NCBI Official Symbol    CHAT [Similar Products]
 NCBI Official Synonym Symbols   
CMS1A; CMS1A2; CHOACTASE
[Similar Products]
 NCBI Protein Information    choline O-acetyltransferase; choline acetylase; acetyl CoA:choline O-acetyltransferase
 Protein Family    (Z)-3-hexen-1-ol acetyltransferase
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 NCBI Summary for CHAT    This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
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 UniProt Comments for CHAT    CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: Acetyltransferase; Lipid Metabolism - glycerophospholipid; EC 2.3.1.6

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cell soma; mitochondrion; axon; cytoplasm; cytosol; nucleus

Molecular Function: choline O-acetyltransferase activity

Biological Process: synaptic transmission; rhythmic excitation; muscle development; rhythmic behavior; phospholipid metabolic process; neurotransmitter secretion; glycerophospholipid biosynthetic process; phosphatidylcholine biosynthetic process; dendrite development; neuromuscular synaptic transmission; establishment of synaptic specificity at neuromuscular junction; adult walking behavior; neurotransmitter biosynthetic process

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
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Product References and Citations for anti-CHAT antibody

   • Karson CN, Casanova MF, Kleinman JE, Griffin WS (1993) Choline acetyltransferase in schizophrenia. Am. J.Psychiatry 150:454-459.
• Holt DJ, Herman MM, Hyde TM, Kleinman JE, Cinton CM, German DC, Hersh LB, Greybiel AM, Saper CB (1999) Evidence for a deficit in cholinergic interneurons in the striatum in schizophrenia. Neuroscience 94(1):21-31.
• Baskin DS, Browning JL, Pirozzolo FJ, Korporaal S, Baskin JA, Appel SH (1999) Brain choline acetyltransferase and mental function in Alzheimer disease. Arch Neurol. 56:1121-1123.
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 Research Articles on CHAT    1. rs1880676 is functional and the allelic variations of this polymorphism are involved in developing nicotine dependence by altering ChAT expression.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-CHAT antibody
 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
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