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anti-CHAT antibody :: Goat Choline acetyltransferase Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420146
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Choline acetyltransferase (CHAT), Polyclonal Antibody

 Also Known As   

Goat anti-Choline acetyltransferase Antibody

 Product Synonym Names    CHAT; choline acetyltransferase; CMS1A; CMS1A2; acetyl CoA: choline O-acetyltransferase; choline acetyltransferase antibody; CHAT antibody; CMS1A antibody; CMS1A2 antibody; acetyl CoA: choline O-acetyltransferase antibody; Choline acetyltransferase; Choline acetyltransferase
 Product Gene Name   

anti-CHAT antibody

[Similar Products]
 Antibody/Peptide Pairs    Choline acetyltransferase peptide (MBS425778) is used for blocking the activity of Choline acetyltransferase antibody (MBS420146)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    CKEKATRPSQ GHQP
 OMIM    118490
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence CKEKATRPSQGHQP, from the C Terminus of the protein sequence according to NP_066264.3; NP_065574.3; NP_001136405.1.
 Epitope    C Terminus
 Note    This antibody is expected to recognise isoform 1 (NP_066264.3), isoform 2 (NP_065574.3) and isoform 3 (NP_001136405.1). Reported variants represent identical protein (NP_066264.3; NP_066265.3; NP_066266.3; NP_001136406.1; NP_001136401.1).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-CHAT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-CHAT antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-CHAT antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 70kDa band observed in Human Placenta lysates (calculated MW of 70.5kDa according to NP_065574 and NP_066264). Recommended concentration: 0. 03-0.3ug/ml.
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 Western Blot (WB) of anti-CHAT antibody    (0. 03ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-CHAT antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for CHAT. It may not necessarily be applicable to this product.
 NCBI GI #    188595668
 NCBI GeneID    1103
 NCBI Accession #    NP_066264.3 [Other Products]
 NCBI Related Accession #    NP_065574.3; NP_001136405.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_020984.3 [Other Products]
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 UniProt Secondary Accession #    Q16488; Q9BQ23; Q9BQ35; Q9BQE1; A2BDF4; A2BDF5 [Other Products]
 UniProt Related Accession #    P28329 [Other Products]
 Molecular Weight    70,394 Da
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 NCBI Official Full Name    choline O-acetyltransferase isoform 1
 NCBI Official Synonym Full Names    choline O-acetyltransferase
 NCBI Official Symbol    CHAT [Similar Products]
 NCBI Official Synonym Symbols   
CMS6; CMS1A; CMS1A2; CHOACTASE
[Similar Products]
 NCBI Protein Information    choline O-acetyltransferase
 UniProt Protein Name    Choline O-acetyltransferase
 Protein Family    (Z)-3-hexen-1-ol acetyltransferase
 UniProt Gene Name    CHAT [Similar Products]
 UniProt Synonym Gene Names    CHOACTase; ChAT; Choline acetylase [Similar Products]
 UniProt Entry Name    CLAT_HUMAN
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 NCBI Summary for CHAT    This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
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 UniProt Comments for CHAT    CHAT: an enzyme that catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. Four alternative splice variants have been described.

Protein type: EC 2.3.1.6; Acetyltransferase; Lipid Metabolism - glycerophospholipid

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: cytoplasm; cytosol; neuron projection; nucleus

Molecular Function: choline binding; choline O-acetyltransferase activity

Biological Process: acetylcholine biosynthetic process; antral ovarian follicle growth; memory; neurotransmitter secretion; phosphatidylcholine biosynthetic process; response to drug; response to ethanol; response to hypoxia; response to nutrient

Disease: Myasthenic Syndrome, Congenital, Associated With Episodic Apnea
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 Research Articles on CHAT    1. There is a striking variability in the severity of phenotypes resulting from mutations in CHAT, which is the only gene so far known to be linked with congenital deficiency of ACh synthesis.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-CHAT antibodyPathways associated with anti-CHAT antibody
 Reference Product  PubMed Publications
 ACHE antibody  >734 publications with CHAT and ACHE
 SLC18A3 antibody  >94 publications with CHAT and SLC18A3
 SLC5A7 antibody  >6 publications with CHAT and SLC5A7
 SLC44A1 antibody  >2 publications with CHAT and SLC44A1
 NTRK1 antibody  >2 publications with CHAT and NTRK1
 VAMP2 antibody  >1 publications with CHAT and VAMP2
 KIFAP3 antibody  >1 publications with CHAT and KIFAP3
 Products by Pathway  Pathway Diagram
 Acetylcholine Neurotransmitter Release Cycle Pathway antibodies  Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
 Acetylcholine Synthesis Pathway antibodies  Acetylcholine Synthesis Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Cholinergic Synapse Pathway antibodies  Cholinergic Synapse Pathway Diagram
 Glycerophospholipid Biosynthesis Pathway antibodies  Glycerophospholipid Biosynthesis Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Glycerophospholipid Metabolism Pathway antibodies  Glycerophospholipid Metabolism Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Lipids And Lipoproteins Pathway antibodies  Metabolism Of Lipids And Lipoproteins Pathway Diagram
 Neuronal System Pathway antibodies  Neuronal System Pathway Diagram
Diseases associated with anti-CHAT antibodyOrgans/Tissues associated with anti-CHAT antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >2638 publications with CHAT and Nervous System Diseases
 Brain Diseases Antibodies  >1404 publications with CHAT and Brain Diseases
 Neurodegenerative Diseases Antibodies  >1064 publications with CHAT and Neurodegenerative Diseases
 Neurobehavioral Manifestations Antibodies  >930 publications with CHAT and Neurobehavioral Manifestations
 Disease Models, Animal Antibodies  >605 publications with CHAT and Disease Models, Animal
 Nerve Degeneration Antibodies  >448 publications with CHAT and Nerve Degeneration
 Memory Disorders Antibodies  >290 publications with CHAT and Memory Disorders
 Movement Disorders Antibodies  >281 publications with CHAT and Movement Disorders
 Cognition Disorders Antibodies  >266 publications with CHAT and Cognition Disorders
 Learning Disorders Antibodies  >249 publications with CHAT and Learning Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >6079 publications with CHAT and Brain
 Eye Antibodies  >577 publications with CHAT and Eye
 Testis Antibodies  >13 publications with CHAT and Testis
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