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anti-MAP1LC3B antibody :: Rabbit Cleaved LC3B Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9205943
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Cleaved LC3B (MAP1LC3B), Polyclonal Antibody

★Popular Item★
 Also Known As   

Cleaved LC3B Antibody

 Product Synonym Names    Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1LC3B; MAP1ALC3
 Product Gene Name   

anti-MAP1LC3B antibody

[Similar Products]
 Antibody/Peptide Pairs    Cleaved LC3B peptide (MBS9223091) is used for blocking the activity of Cleaved LC3B antibody (MBS9205943)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    89-122
 OMIM    609604
 3D Structure    ModBase 3D Structure for Q9GZQ8
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human, mouse (Predicted Reactivity: Bovine)
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 Specificity    This Cleaved LC3B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-122 amino acids from human Cleaved LC3B.
 Purity/Purification    Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.25 (lot specific)
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 Antigen Type    Synthetic Peptide
 Other Accession    O41515
 Antigen Source    HUMAN
  Function    Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
 Cellular Location    Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes Localizes also to discrete punctae along the ciliary axoneme (By similarity).
 Tissue Location    Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-MAP1LC3B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAP1LC3B antibody

   Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. These proteins are involved in formation of autophagosomal vacuoles (autophagosomes). MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. MAP1LC3b is one of the light chain subunits and can associate with either MAP1A or MAP1B. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
 Product Categories/Family for anti-MAP1LC3B antibody    Cancer; Cardiovascular; Metabolism; Neuroscience; Signal Transduction; Autophagy
 Applications Tested/Suitable for anti-MAP1LC3B antibody   

Immunofluorescence (IF), ELISA (EIA), Immunocytochemistry (ICC)

 Application Notes for anti-MAP1LC3B antibody    IF~~1:10~50
ICC~~1:10~50
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 Immunofluorescence (IF) of anti-MAP1LC3B antibody    Fluorescent image of U251 cells stained with cleaved LC3B antibody. U251 cells were treated with Chloroquine (50 uM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9205943 cleaved LC3B primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). LC3 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
anti-MAP1LC3B antibody Immunofluorescence (IF) image
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 Immunofluorescence (IF) of anti-MAP1LC3B antibody    Time course study of mouse leukaemic monocyte macrophage cells treated with U18666A, a drug that causes cholesterol and lipid storage in cells, thereby blocking fusion between late endosomes and lysosomes. Cleaved-LC3 (APG8b) antibody detected punctuate staining indicative of autophagic vacuole or phagosome structures. Data courtesy of Dr. Barry Boland, Department of Pharmacology, Oxford University.
anti-MAP1LC3B antibody Immunofluorescence (IF) image
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 Immunocytochemistry (ICC) of anti-MAP1LC3B antibody    SY5Y cells were pretreated with 5nM bafilomycin for 24hr and fixed in methanol (left panel) or 4% of paraformaldehyde (right panel). Treatment with antibody at dilution 1:100. Data courtesy of Jianhui Zhu, MD, PhD & Charleen T. Chu, MD, PhD, University of Pittsburgh School of Medicine.
anti-MAP1LC3B antibody Immunocytochemistry (ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for MAP1LC3B. It may not necessarily be applicable to this product.
 NCBI GI #    12383056
 NCBI GeneID    81631
 NCBI Accession #    NP_073729.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_022818.4 [Other Products]
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 UniProt Primary Accession #    Q9GZQ8 [Other Products]
 UniProt Secondary Accession #    Q6NW02 [Other Products]
 UniProt Related Accession #    Q9GZQ8 [Other Products]
 Molecular Weight    14688
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 NCBI Official Full Name    microtubule-associated proteins 1A/1B light chain 3B
 NCBI Official Synonym Full Names    microtubule-associated protein 1 light chain 3 beta
 NCBI Official Symbol    MAP1LC3B [Similar Products]
 NCBI Official Synonym Symbols   
LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
[Similar Products]
 NCBI Protein Information    microtubule-associated proteins 1A/1B light chain 3B
 UniProt Protein Name    Microtubule-associated proteins 1A/1B light chain 3B
 UniProt Synonym Protein Names   
Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
 Protein Family    Microtubule-associated protein
 UniProt Gene Name    MAP1LC3B [Similar Products]
 UniProt Synonym Gene Names    MAP1ALC3; MAP1A/MAP1B LC3 B [Similar Products]
 UniProt Entry Name    MLP3B_HUMAN
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 NCBI Summary for MAP1LC3B    The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
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 UniProt Comments for MAP1LC3B    LC3B: is a ubiquitin-like protein that is a constituent of the ATG8-conjugation system, one of two evolutionarily conserved phosphatidylethanolamine conjugation systems necessary for the formation of the autophagosome. The human ATG8 system includes seven ubiquitin-like light chain proteins (LCPs) that are homologs of yeast LC3: MAP1LC3A, -B, -C, GABARAP, GABARAPL1, -2, and -3. Pro-LCPs are cleaved by ATG4B to expose a C-terminal glycine residue, the cytosolic LCP-I form. The exposed C-terminus is conjugated to the head group amine of phosphatidylethanolamine (PE) through an amide bond by a sequence of ubiquitination-like reactions that involves an E1 (ATG7), an E2 (ATG3), and an E3 (a complex including ATG5, ATG12, and ATG16L). The PE-congugated form (LCP-II) is tightly associated with the autophagosomal membrane. The LCP-II forms can also be delipidated by the ATG4 proteases: most of the LCPs are delipidated and liberated from the membrane before autophagosomes fuse with lysosomes. Interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. Recruits proteins such as SQSTM1 and NBR1 to the autophagosome.

Protein type: Vesicle; Ubiquitin-like modifier; Autophagy; Microtubule-binding

Chromosomal Location of Human Ortholog: 16q24.2

Cellular Component: microtubule; extrinsic to membrane; mitochondrion; endomembrane system; autophagic vacuole; intracellular; cytoplasmic vesicle; axoneme; cytosol

Molecular Function: protein binding; microtubule binding; GABA receptor binding

Biological Process: mitochondrion degradation; autophagic vacuole formation; cellular response to nitrogen starvation
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Product References and Citations for anti-MAP1LC3B antibody

   References for protein:
1.Baehrecke EH. Nat Rev Mol Cell Biol. 6(6):505-10. (2005)
2.Lum JJ, et al. Nat Rev Mol Cell Biol. 6(6):439-48. (2005)
3.Greenberg JT. Dev Cell. 8(6):799-801. (2005)
4. Levine B. Cell. 120(2):159-62. (2005)
5.Shintani T and Klionsky DJ. Science. 306(5698):990-5. (2004)
6.Tanida I., et al. Int. J. Biochem. Cell Biol. 36:2503-2518(2004)
7.He H., et al. J. Biol. Chem. 278:29278-29287(2003)
8.Tanida I., et al. J. Biol. Chem. 279:36268-36276(2004)
References for U251 cell line:
1. Westermark B.; PontÚn J.; Hugosson R. (1973)." Determinants for the establishment of permanent tissue culture lines from human gliomas". Acta Pathol Microbiol Scand A. 81:791-805. [PMID: 4359449].
2. PontÚn, J.,Westermark B. (1978)." Properties of Human Malignant Glioma Cells in Vitro". Medical Biology 56: 184-193.[PMID: 359950].
3. Geng Y.;Kohli L.; Klocke B.J.; Roth K.A.(2010). "Chloroquine-induced autophagic vacuole accumulation and cell death in glioma cells is p53 independent". Neuro Oncol. 12(5): 473-481.[ PMID: 20406898].

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 Research Articles on MAP1LC3B    1. investigated the expression of autophagy-related markers microtubule-associated protein IA/IB light chain 3 (LC3) and p62/sequestosome-1 (p62), in cutaneous squamous cell carcinoma specimens and assessed their correlation to clinicopathological factors
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAP1LC3B antibodyPathways associated with anti-MAP1LC3B antibody
 Reference Product  PubMed Publications
 SQSTM1 antibody  >53 publications with MAP1LC3B and SQSTM1
 ATG5 antibody  >31 publications with MAP1LC3B and ATG5
 ATG7 antibody  >23 publications with MAP1LC3B and ATG7
 ATG12 antibody  >14 publications with MAP1LC3B and ATG12
 ULK1 antibody  >11 publications with MAP1LC3B and ULK1
 BNIP3 antibody  >5 publications with MAP1LC3B and BNIP3
 ATG3 antibody  >5 publications with MAP1LC3B and ATG3
 ATG4B antibody  >5 publications with MAP1LC3B and ATG4B
 ATG4A antibody  >1 publications with MAP1LC3B and ATG4A
 Products by Pathway  Pathway Diagram
 Nitrotoluene Degradation Pathway antibodies  Nitrotoluene Degradation Pathway Diagram
Diseases associated with anti-MAP1LC3B antibodyOrgans/Tissues associated with anti-MAP1LC3B antibody
 Disease Name  Pubmed Publications
 Breast Neoplasms Antibodies  >19 publications with MAP1LC3B and Breast Neoplasms
 Nervous System Diseases Antibodies  >15 publications with MAP1LC3B and Nervous System Diseases
 Necrosis Antibodies  >15 publications with MAP1LC3B and Necrosis
 Inflammation Antibodies  >10 publications with MAP1LC3B and Inflammation
 Neoplasms, Experimental Antibodies  >7 publications with MAP1LC3B and Neoplasms, Experimental
 Neoplasm Metastasis Antibodies  >7 publications with MAP1LC3B and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >6 publications with MAP1LC3B and Lung Neoplasms
 Carcinoma, Squamous Cell Antibodies  >5 publications with MAP1LC3B and Carcinoma, Squamous Cell
 Fibrosis Antibodies  >5 publications with MAP1LC3B and Fibrosis
 Liver Diseases Antibodies  >4 publications with MAP1LC3B and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >18 publications with MAP1LC3B and Lung
 Blood Antibodies  >17 publications with MAP1LC3B and Blood
 Brain Antibodies  >12 publications with MAP1LC3B and Brain
 Kidney Antibodies  >10 publications with MAP1LC3B and Kidney
 Skin Antibodies  >9 publications with MAP1LC3B and Skin
 Muscle Antibodies  >8 publications with MAP1LC3B and Muscle
 Lymph Node Antibodies  >7 publications with MAP1LC3B and Lymph Node
 Lymph Antibodies  >7 publications with MAP1LC3B and Lymph
 Liver Antibodies  >7 publications with MAP1LC3B and Liver
 Prostate Antibodies  >6 publications with MAP1LC3B and Prostate
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