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anti-F8 antibody :: Rabbit anti-Human Coagulation Factor VIII (F8) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2002060 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $105 +1 FREE 8GB USB
0.02 mg  /  $125 +1 FREE 8GB USB
0.05 mg  /  $170 +1 FREE 8GB USB
0.1 mg  /  $220 +1 FREE 8GB USB
0.2 mg  /  $320 +1 FREE 8GB USB
1 mg  /  $760 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Coagulation Factor VIII (F8), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to Coagulation Factor VIII (F8)

 Product Gene Name   

anti-F8 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-GRQV TVQEFALFFT IFDETKSWYF TENMERNCRA PCNIQMEDPT FKENYRFHAI NGYIMDTLPG LVMAQDQRIR WYLLSMGSNE NIHSIHFSGH VFTVRKKEEY
 OMIM    M14113 mRNA
 3D Structure    ModBase 3D Structure for P00451
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Specificity    The antibody is a rabbit polyclonal antibody raised against F8. It has been selected for its ability to recognize F8 in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Recombinant F8 (Gly1887~Leu2044) expressed in E.coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2060195
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-F8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-F8 antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

 Application Notes for anti-F8 antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) of anti-F8 antibody    (Figure. Western Blot; Sample: Recombinant protein.)
anti-F8 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-F8 antibody    (DAB staining on IHC-P; Samples: Human Cerebrum Tissue)
anti-F8 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-F8 antibody    (Used in DAB staining on formalin fixed paraffin- embedded glioma tissue)
anti-F8 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for F8. It may not necessarily be applicable to this product.
 NCBI GI #    4503647
 NCBI GeneID    2157
 NCBI Accession #    NP_000123.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000132.3 [Other Products]
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 UniProt Primary Accession #    P00451 [Other Products]
 UniProt Secondary Accession #    Q14286; Q5HY69 [Other Products]
 UniProt Related Accession #    P00451 [Other Products]
 Molecular Weight    24,641 Da
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 NCBI Official Full Name    coagulation factor VIII isoform a preproprotein
 NCBI Official Synonym Full Names    coagulation factor VIII
 NCBI Official Symbol    F8 [Similar Products]
 NCBI Official Synonym Symbols   
AHF; F8B; F8C; HEMA; FVIII; DXS1253E
[Similar Products]
 NCBI Protein Information    coagulation factor VIII
 UniProt Protein Name    Coagulation factor VIII
 UniProt Synonym Protein Names   
Antihemophilic factor; AHF
 Protein Family    Coagulation factor
 UniProt Gene Name    F8 [Similar Products]
 UniProt Synonym Gene Names    F8C; AHF [Similar Products]
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 NCBI Summary for F8    This gene encodes coagulation factor VIII, which participates in the intrinsic pathway of blood coagulation; factor VIII is a cofactor for factor IXa which, in the presence of Ca+2 and phospholipids, converts factor X to the activated form Xa. This gene produces two alternatively spliced transcripts. Transcript variant 1 encodes a large glycoprotein, isoform a, which circulates in plasma and associates with von Willebrand factor in a noncovalent complex. This protein undergoes multiple cleavage events. Transcript variant 2 encodes a putative small protein, isoform b, which consists primarily of the phospholipid binding domain of factor VIIIc. This binding domain is essential for coagulant activity. Defects in this gene results in hemophilia A, a common recessive X-linked coagulation disorder. [provided by RefSeq, Jul 2008]
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 UniProt Comments for F8    F8: Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa. Defects in F8 are the cause of hemophilia A (HEMA). A disorder of blood coagulation characterized by a permanent tendency to hemorrhage. About 50% of patients have severe hemophilia resulting in frequent spontaneous bleeding into joints, muscles and internal organs. Less severe forms are characterized by bleeding after trauma or surgery. Of particular interest for the understanding of the function of F8 is the category of CRM (cross-reacting material) positive patients (approximately 5%) that have considerable amount of F8 in their plasma (at least 30% of normal), but the protein is non- functional; i.e. the F8 activity is much less than the plasma protein level. CRM-reduced is another category of patients in which the F8C antigen and activity are reduced to approximately the same level. Most mutations are CRM negative, and probably affect the folding and stability of the protein. Belongs to the multicopper oxidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: COPII-coated ER to Golgi transport vesicle; endoplasmic reticulum lumen; endoplasmic reticulum-Golgi intermediate compartment membrane; extracellular region; extracellular space; Golgi membrane; plasma membrane

Molecular Function: copper ion binding; oxidoreductase activity; protein binding

Biological Process: acute-phase response; blood coagulation; blood coagulation, intrinsic pathway; COPII vesicle coating; ER to Golgi vesicle-mediated transport; platelet activation; platelet degranulation

Disease: Hemophilia A
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 Research Articles on F8    1. Potential mutations of the F8 gene were analyzed.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-F8 antibodyPathways associated with anti-F8 antibody
 Reference Product  PubMed Publications
 VWF antibody  >58 publications with F8 and VWF
 F10 antibody  >26 publications with F8 and F10
 LMAN1 antibody  >3 publications with F8 and LMAN1
 SERPINE1 antibody  >2 publications with F8 and SERPINE1
 Products by Pathway  Pathway Diagram
 Blood Clotting Cascade Pathway antibodies  Blood Clotting Cascade Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Complement And Coagulation Cascades Pathway antibodies  Complement And Coagulation Cascades Pathway Diagram
 Formation Of Fibrin Clot (Clotting Cascade) Pathway antibodies  Formation Of Fibrin Clot (Clotting Cascade) Pathway Diagram
 Hemostasis Pathway antibodies  Hemostasis Pathway Diagram
 Intrinsic Pathway antibodies  Intrinsic Pathway Diagram
 Platelet Activation, Signaling And Aggregation Pathway antibodies  Platelet Activation, Signaling And Aggregation Pathway Diagram
 Platelet Degranulation Pathway antibodies  Platelet Degranulation Pathway Diagram
 Response To Elevated Platelet Cytosolic Ca2+ Pathway antibodies  Response To Elevated Platelet Cytosolic Ca2+ Pathway Diagram
Diseases associated with anti-F8 antibodyOrgans/Tissues associated with anti-F8 antibody
 Disease Name  Pubmed Publications
 Hemophilia A Antibodies  >507 publications with F8 and Hemophilia A
 Hemorrhage Antibodies  >134 publications with F8 and Hemorrhage
 Neoplasms Antibodies  >112 publications with F8 and Neoplasms
 Nervous System Diseases Antibodies  >100 publications with F8 and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >87 publications with F8 and Cardiovascular Diseases
 Thrombosis Antibodies  >69 publications with F8 and Thrombosis
 Brain Diseases Antibodies  >47 publications with F8 and Brain Diseases
 Heart Diseases Antibodies  >29 publications with F8 and Heart Diseases
 Kidney Diseases Antibodies  >27 publications with F8 and Kidney Diseases
 Venous Thrombosis Antibodies  >26 publications with F8 and Venous Thrombosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >140 publications with F8 and Brain
 Muscle Antibodies  >55 publications with F8 and Muscle
 Kidney Antibodies  >54 publications with F8 and Kidney
 Bone Antibodies  >42 publications with F8 and Bone
 Liver Antibodies  >37 publications with F8 and Liver
 Nerve Antibodies  >26 publications with F8 and Nerve
 Lung Antibodies  >24 publications with F8 and Lung
 Skin Antibodies  >23 publications with F8 and Skin
 Eye Antibodies  >23 publications with F8 and Eye
 Spleen Antibodies  >16 publications with F8 and Spleen
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