Product Name
Collagen Type IV (COL4), Polyclonal Antibody
Popular Item
Full Product Name
Collagen Type IV antibody (biotin)
Product Synonym Names
Polyclonal Collagen Type IV; Anti-Collagen Type IV; Collagen Of Basement Membrane alpha 1 Chain; Canstatin; Arresten
Research Use Only
For Research Use Only. Not for use in diagnostic procedures.
Species Reactivity
Human, Bovine
Specificity
Bovine/Human collagen type IV
Purity/Purification
Collagen Type IV antibody (biotin) was purified by affinity chromatography.
Form/Format
Lyophilized from 20mM Potassium Phosphate, pH 7.2, with 150mM Sodium Chloride, 10mg/ml BSA as a stabilizer (Immunoglobulin and Protease free), and 0.01% Sodium Azide added as a preservative. Reconstitute with 0.1ml of deionized water (or equivalent).
Concentration
100ug, lyophilized (lot specific)
Immunogen
Collagen type IV from human and bovine placenta
Species Reactivity Note
This antibody reacts with most mammalian Type IV collagens and has negligible cross reactivity with Type I, II, III, IV collagens. Non specific cross reaction with other human serum proteins or non-collagen extracellular matrix proteins is negligible.
Bioactivity
Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Preparation and Storage
Ships ambient or refrigerated. Upon receipt store at 4°C while lyophilized. After reconstituted, store at 4°C short term, or aliquot and store at -20°C long term. Avoid repeat freeze-thaws.
Other Notes
Small volumes of anti-COL4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
|
Related Product Information for
anti-COL4 antibody
Collagen is a group of naturally occurring proteins found, in nature, exclusively in animals, especially in the flesh and connective tissues of mammals. It is the main component of connective tissue, and is the most abundant protein in mammals, making up about 25% to 35% of the whole-body protein content. Type-IV collagen is a type of collagen found primarily in the basal lamina. The C-terminus domain is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, type-IV lacks the regular glycine in every third residue necessary for the tight, collagen helix.
|
Applications Tested/Suitable for
anti-COL4 antibody
ELISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes for
anti-COL4 antibody
ELISA: 1:20,000-1:100,000
IHC: 1:1,000-1:5,000
WB: 1:2,000-1:10,000
Immunohistochemistry of
anti-COL4 antibody
Immunohistochemical staining using Collagen Type IV antibody
Anti collagen IV antibody (1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli and liver (Right) with strong staining in sinusoids. Staining for both tissues was consistent with a basement membrane distribution. Slides were steamed in 0. 01 M sodium citrate buffer, pH 6. 0 at 99-100 deg C - 20 minutes for antigen retrieval.
NCBI Summary for COL4
This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
UniProt Comments for COL4
COL4A5: Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A5 are the cause of Alport syndrome X- linked (APSX). APSX is characterized by progressive glomerulonephritis, renal failure, sensorineural deafness, specific eye abnormalities (lenticonous and macular flecks), and glomerular basement membrane defects. The disorder shows considerable heterogeneity in that families differ in the age of end-stage renal disease and the occurrence of deafness. Deletions covering the N-terminal regions of COL4A5 and COL4A6, which are localized in a head-to-head manner, are found in the chromosome Xq22.3 centromeric deletion syndrome. This results in a phenotype with features of diffuse leiomyomatosis and Alport syndrome (DL-ATS). Belongs to the type IV collagen family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Extracellular matrix; Secreted
Chromosomal Location of Human Ortholog: Xq22
Cellular Component: endoplasmic reticulum lumen; collagen type IV; extracellular region; basal lamina; neuromuscular junction
Molecular Function: extracellular matrix structural constituent
Biological Process: extracellular matrix disassembly; collagen catabolic process; axon guidance; extracellular matrix organization and biogenesis; neuromuscular junction development
Disease: Alport Syndrome, X-linked
Research Articles on COL4
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our
Terms & Conditions page for more information.
Diseases associated with anti-COL4 antibody