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anti-GJA1 antibody :: Rabbit anti-Rat Connexin 43 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS502014
Testing Data
Unit / Price
0.1 mL  /  $360 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Connexin 43 (GJA1), Polyclonal Antibody

 Also Known As   

Anti-Connexin43

 Product Gene Name   

anti-GJA1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 20; NC_005119.3 (39612080..39624555). Location: 20q11
 3D Structure    ModBase 3D Structure for P08050
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Rat
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 Specificity    Specific for the ~43k connexin43 protein.
 Purity/Purification    Affinity Purified (Prepared from rabbit serum by affinity purification on a column made with the C-terminal peptide used as antigen.)
 Form/Format    100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
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 Antigen    Peptide corresponding to amino acid residues from the C-terminal region of rat connexin43.
 Immunogen Information    Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
 Immunogen Species    Rat
 Reactivity Assumed Based on 100% Sequence Homology    Chicken, canine, finch, human, mouse non-human primate, sheep Xenopus and zebra fish
 Species Reactivity Note    The antibody has been directly tested for reactivity in Western blots in rat tissues. It is anticipated that the antibody will also work with chicken, dog, finch, human, mouse, non-human primate, sheep, Xenopus and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
 Biological Significance    Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at er368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b).
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 Preparation and Storage    Store at -20 degree C; stable for at least one year.
 Other Notes    Small volumes of anti-GJA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GJA1 antibody

   Affinity purified rabbit polyclonal antibody
 Applications Tested/Suitable for anti-GJA1 antibody   

Western Blot (WB)

 Application Notes for anti-GJA1 antibody    Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: Not recommended
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 Testing Data of anti-GJA1 antibody    anti-GJA1 antibody Testing Data image
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NCBI/Uniprot data below describe general gene information for GJA1. It may not necessarily be applicable to this product.
 NCBI GI #    6978896
 NCBI GeneID    24392
 NCBI Accession #    NP_036699.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012567.2 [Other Products]
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 UniProt Primary Accession #    P08050 [Other Products]
 UniProt Secondary Accession #    Q53ZE1 [Other Products]
 UniProt Related Accession #    P08050 [Other Products]
 Molecular Weight    43
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 NCBI Official Full Name    gap junction alpha-1 protein
 NCBI Official Synonym Full Names    gap junction protein, alpha 1
 NCBI Official Symbol    Gja1 [Similar Products]
 NCBI Official Synonym Symbols   
Cx43
[Similar Products]
 NCBI Protein Information    gap junction alpha-1 protein; connexin 43; connexin-43; gap junction 43 kDa heart protein; gap junction membrane channel protein alpha 1
 UniProt Protein Name    Gap junction alpha-1 protein
 UniProt Synonym Protein Names   
Connexin-43; Cx43; Gap junction 43 kDa heart protein
 Protein Family    Gap junction alpha-1 protein
 UniProt Gene Name    Gja1 [Similar Products]
 UniProt Synonym Gene Names    Cxn-43; Cx43 [Similar Products]
 UniProt Entry Name    CXA1_RAT
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 NCBI Summary for GJA1    gap junction component; plays a role in cell-cell communication [RGD, Feb 2006]
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 UniProt Comments for GJA1    Function: Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract

By similarity.

Subunit structure: A connexon is composed of a hexamer of connexins. Interacts with SGSM3. Interacts with KIAA1432/CIP150. Interacts with CNST and CSNK1D

By similarity. Interacts (via C-terminus) with TJP1. Interacts (via C-terminus) with SRC (via SH3 domain). Interacts with UBQLN4. Ref.10 Ref.12 Ref.13

Subcellular location: Cell membrane; Multi-pass membrane protein. Cell junction › gap junction Ref.13.

Induction: In bladder smooth muscle cells, exhibits night/day variations with low levels during the sleep phase, at circadian time (CT) 4-8 (at protein level). Expression starts to increase around CT12 and forms a plateau during the active phase (CT16-24) (at protein level). Ref.11

Post-translational modification: Contains at least one intramolecular disulfide bond.Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity

By similarity. Ref.9Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2

By similarity.S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.

Sequence similarities: Belongs to the connexin family. Alpha-type (group II) subfamily.
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Product References and Citations for anti-GJA1 antibody

   • Aronica E, Gorter JA, Jansen GH, Leenstra S, Yankaya B, Troost D (2001) Expression of connexin 43 and connexin 32 gap-junction proteins in epilepsy-associated brain tumors and in the perilesional epileptic cortex. Acta Neuropathol (Berl) 101:449-459.
• Bao X, Altenberg GA, Reuss L (2004a) Mechanism of regulation of the gap junction protein connexin 43 by protein kinase C-mediated phosphorylation. Am J Physiol Cell Physiol 286:C647-C654.
• Bao X, Reuss L, Altenberg GA (2004b) Regulation of purified and reconstituted connexin 43 hemichannels by protein kinase C-mediated phosphorylation of Serine 368. J Biol Chem 279:20058-20066.
• Yogo K, Ogawa T, Akiyama M, Ishida N, Takeya T (2002) Identification and functional analysis of novel phosphorylation sites in Cx43 in rat primary granulosa cells. FEBS Lett 531:132-136.
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 Research Articles on GJA1    1. Data indicate that Gja1, Cav3 and Kcnk3 were up-regulated and Hcn2 was down-regulated in hearts of exercise trained Goto-Kakizaki (GK) type 2 diabetic rat compared to sedentary GK controls.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-GJA1 antibodyDiseases associated with anti-GJA1 antibody
 Products by Pathway  Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway antibodies  Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway Diagram
 Calcium Regulation In The Cardiac Cell Pathway antibodies  Calcium Regulation In The Cardiac Cell Pathway Diagram
 EGFR1 Signaling Pathway antibodies  EGFR1 Signaling Pathway Diagram
 Formation Of Annular Gap Junctions Pathway antibodies  Formation Of Annular Gap Junctions Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Assembly Pathway antibodies  Gap Junction Assembly Pathway Diagram
 Gap Junction Degradation Pathway antibodies  Gap Junction Degradation Pathway Diagram
 Gap Junction Trafficking Pathway antibodies  Gap Junction Trafficking Pathway Diagram
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >69 publications with GJA1 and Cardiovascular Diseases
 Heart Diseases Antibodies  >62 publications with GJA1 and Heart Diseases
 Oculodentodigital Dysplasia Antibodies  >53 publications with GJA1 and Oculodentodigital Dysplasia
 Neoplasms Antibodies  >44 publications with GJA1 and Neoplasms
 Arrhythmias, Cardiac Antibodies  >39 publications with GJA1 and Arrhythmias, Cardiac
 Disease Models, Animal Antibodies  >28 publications with GJA1 and Disease Models, Animal
 Fibrosis Antibodies  >11 publications with GJA1 and Fibrosis
 Infertility, Male Antibodies  >10 publications with GJA1 and Infertility, Male
 Inflammation Antibodies  >8 publications with GJA1 and Inflammation
 Breast Neoplasms Antibodies  >7 publications with GJA1 and Breast Neoplasms
Organs/Tissues associated with anti-GJA1 antibody
 Organ/Tissue Name  Pubmed Publications
 Heart Antibodies  >95 publications with GJA1 and Heart
 Muscle Antibodies  >86 publications with GJA1 and Muscle
 Eye Antibodies  >53 publications with GJA1 and Eye
 Brain Antibodies  >33 publications with GJA1 and Brain
 Ovary Antibodies  >18 publications with GJA1 and Ovary
 Embryonic Tissue Antibodies  >15 publications with GJA1 and Embryonic Tissue
 Lung Antibodies  >12 publications with GJA1 and Lung
 Kidney Antibodies  >10 publications with GJA1 and Kidney
 Liver Antibodies  >7 publications with GJA1 and Liver
 Connective Tissue Antibodies  >6 publications with GJA1 and Connective Tissue
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