View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List D-5 Dopamine receptor IgG fraction Polyclonal Antibody Catalog # MBS352077 Unit / Price 0.1 mL / $735 +1 FREE 8GB USB 5x0.1 mL / $3,200 +4 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name D-5 Dopamine receptor IgG fraction, Polyclonal Antibody Full Product Name D-5 Dopamine receptor IgG fraction Product Synonym Names D 5 (23-35); D5 Dopamine Receptor; IgG Fraction of Rabbit Anti-D5 Receptor Sera Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Specificity Polypeptide ::: % Cross ReactivityD5 Dopamine Receptor ::: ~80 Form/Format Antiserum Specificity Other Notes Small volumes of anti-D-5 Dopamine receptor IgG fraction antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-D-5 Dopamine receptor IgG fraction antibody The antiserum was raised in a rabbit which was immunized with D5(Ac-23-35-Cys36) covalently attached onto a carrier protein. and it has been characterized by immunocytochemical. western immunoblot and ELISA techniques. The antiserum and the IgG fraction of the antiserum have been found to be highly specific for this peptide sequence and are suitable for immunocytochemical and western blot detection of the D5 dopamine receptor. Rehydrate the lyophilized IgG fraction with 0.1 ml of PBS which contains 10 mg/ml BSA or with additional buffer (such as 10 mg/ml BSA in PBS) for more dilute antibody. Each vial contains 2.19 mg of purified rabbit IgG. Product Categories/Family for anti-D-5 Dopamine receptor IgG fraction antibody Histochemical Grade Antibodies; Dopamine Receptor Antibodies Applications Tested/Suitable for anti-D-5 Dopamine receptor IgG fraction antibody Immunocytochemical Staining, Western Immunoblot, Western Blot Application Notes for anti-D-5 Dopamine receptor IgG fraction antibody Immunocytochemistry: This IgG fraction has been found to stain specific cells in various regions of PLP fixed rat brain sections at a concentration of 10 ug/ml. This includes the Medial septum. Nucleus accumbens. Dentate gyrus. Globus pallidus. Medial forebrain bundle. Cortex regions 1-3. Substantia Nigra reticulata. and the Ventral tegmental area..Western Blot: Western immunoblots using whole rat brain homogenate resulted in a single band being detected at ~49 KDa at a concentration of 100 ug/ml.Western Blot Protocol: 1. After SDS-PAGE (on either 4-15% gradient gels or single percentage gels. such at 12% gels) and electrophoretic transfer to PVDF membrane. block the membrane overnight with 4% normal goat serum in TBS/Tween-20 buffer.2. Wash x 2 with TBS/Tween-20.3. Apply the IgG fraction after dilution to at least 100 ug/ml (Note: higher dilutions may be needed). Use 2% normal goat serum in TBS/Tween-20 as buffer for the primary antibody. Let the primary antibody bind for 2-4 hours.4. Wash x 3 with TBS/Tween-20.5. Apply affinity purified HRP-goat anti-rabbit IgG antiserum diluted 1:2500 (dilution may vary depending upon supplier) in 2% normal goat serum in TBS/Tween-20. Incubate 1-2 hours. Note: greater sensitivity may be achieved using ABC techniques.6. Wash x 4 for 5 minute/wash with TBS/Tween-20.7. Develop color using the enhanced DAB reaction. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP