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| Product Name
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DAB2, Polyclonal Antibody ★Popular Item★
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| Also Known As
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DAB2 Rabbit Polyclonal
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| Product Synonym Names
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DAB2, Disabled homolog 2, DOC 2, DOC2, FLJ26626
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| Product Gene Name
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[Similar Products]
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| OMIM
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601236
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| 3D Structure
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ModBase 3D Structure for P98082
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| Clonality
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Polyclonal
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| Isotype
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IgG
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| Host
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Rabbit
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| Species Reactivity
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Human, Mouse, Rat
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| Specificity
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Human, Mouse ,Rat; other species are not tested. Please decide the specificity by homology
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| Purity/Purification
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Purification: Immunogen affinity purified
Purity: > or =95% as determined by SDS-PAGE
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| Form/Format
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Liquid
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| Immunogen
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Disabled homolog 2, mitogen-responsive phosphoprotein (Drosophila)
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| Buffer
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PBS with 0.02% sodium azide and 50% glycerol pH 7.3
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| Preparation and Storage
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Store at -20 degree C for 24 months (Avoid repeated freeze/thaw cycles.)
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| ISO Certification
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Manufactured in an ISO 9001:2008 Certified Laboratory.
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| Other Notes
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Small volumes of anti-DAB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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TOP
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Adapter protein that functions as clathrin-associated sorting protein (CLASP) required for clathrin-mediated endocytosis of selected cargo proteins. Can bind and assemble clathrin, and binds simultaneously to phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) and cargos containg non-phosphorylated NPXY internalization motifs, such as the LDL receptor, to recruit them to clathrin-coated pits. Can function in clathrin-mediated endocytosis independently of the AP-2 complex. Involved in endocytosis of integrin beta-1; this function seems to redundant with the AP-2 complex and seems to require DAB2 binding to endocytosis accessory EH domain-containing proteins such as EPS15, EPS15L1 and ITSN1. Involved in endocytosis of cystic fibrosis transmembrane conductance regulator/CFTR.
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| Applications Tested/Suitable for anti-DAB2 antibody
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ELISA (EIA), Western Blot (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
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| Application Notes for anti-DAB2 antibody
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WB : 1:500-1:5000
IP : 1:1000-1:10000
IF : 1:20-1:200
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| Immunofluorescent of anti-DAB2 antibody
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Immunofluorescent analysis of HepG2 cells, using DAB2 antibody MBS768460 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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| SDS-PAGE of anti-DAB2 antibody
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HeLa cells were subjected to SDS PAGE followed by western blot with MBS768460 (DAB2 antibody) at dilution of 1:1000
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TOP
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| NCBI/Uniprot data below describe general gene information for DAB2. It may not necessarily be applicable to this product. |
| NCBI GI #
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349585060
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| NCBI GeneID
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1601
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| NCBI Accession #
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NP_001231800.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_001244871.1
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[Other Products]
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| UniProt Primary Accession #
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P98082
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[Other Products]
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| UniProt Secondary Accession #
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Q13598; Q9BTY0; Q9UK04; A6NES5
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[Other Products]
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| UniProt Related Accession #
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P98082
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[Other Products]
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| Molecular Weight
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96 kDa
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TOP
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| NCBI Official Full Name
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disabled homolog 2 isoform 2
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| NCBI Official Synonym Full Names
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DAB2, clathrin adaptor protein
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| NCBI Official Symbol
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DAB2
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[Similar Products]
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| NCBI Official Synonym Symbols
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DOC2; DOC-2
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[Similar Products]
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| NCBI Protein Information
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disabled homolog 2
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| UniProt Protein Name
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Disabled homolog 2
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| UniProt Synonym Protein Names
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Adaptor molecule disabled-2; Differentially expressed in ovarian carcinoma 2; DOC-2; Differentially-expressed protein 2
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| Protein Family
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Disabled
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| UniProt Gene Name
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DAB2
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[Similar Products]
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| UniProt Synonym Gene Names
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DOC2; DOC-2
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[Similar Products]
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| UniProt Entry Name
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DAB2_HUMAN
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| NCBI Summary for DAB2
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This gene encodes a mitogen-responsive phosphoprotein. It is expressed in normal ovarian epithelial cells, but is down-regulated or absent from ovarian carcinoma cell lines, suggesting its role as a tumor suppressor. This protein binds to the SH3 domains of GRB2, an adaptor protein that couples tyrosine kinase receptors to SOS (a guanine nucleotide exchange factor for Ras), via its C-terminal proline-rich sequences, and may thus modulate growth factor/Ras pathways by competing with SOS for binding to GRB2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]
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| UniProt Comments for DAB2
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DAB2: an adaptor protein that interacts with Grb2, myosin VI, SMAD2/3, DIP1/2, Dvl-3, the integrin betasubunit, and c-Src. Negatively regulates canonical Wnt signaling by stabilizing the beta-catenin degradation complex. A component of the CSF-1 signal transduction pathway. Phosphorylated DAB2 inhibits adhesion and integrin signaling. Two alternatively spliced isoforms are described.
Protein type: Tumor suppressor; Adaptor/scaffold
Chromosomal Location of Human Ortholog: 5p13.1
Cellular Component: clathrin coated vesicle membrane; clathrin-coated vesicle; coated pit; focal adhesion; intracellular membrane-bound organelle; lysosomal membrane; nucleolus; plasma membrane
Molecular Function: protein binding; protein C-terminus binding; SMAD binding
Biological Process: apoptosis; cell proliferation; integrin-mediated signaling pathway; leading edge cell differentiation; multicellular organismal development; negative regulation of apoptosis; negative regulation of protein binding; negative regulation of transcription, DNA-dependent; positive regulation of cell migration; positive regulation of endocytosis; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway; protein transport; receptor-mediated endocytosis; Wnt receptor signaling pathway
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TOP
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| Research Articles on DAB2
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1. DAB2 regulated the cell migration associated genes in PC3 cells, and the differential DAB2 expression between LNCaP and PC3 cells was partly regulated by histone 4 acetylation.
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TOP
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Products associated with anti-DAB2 antibody | Pathways associated with anti-DAB2 antibody |
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| Diseases associated with anti-DAB2 antibody | Organs/Tissues associated with anti-DAB2 antibody |
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TOP
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Antibody 
