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anti-DAD1 antibody :: Rabbit anti-Human, Mouse DAD1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS151036
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DAD1, Polyclonal Antibody

 Also Known As   

DAD1 Antibody

 Product Synonym Names    DAD1; OST2; Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; Defender against cell death 1; Oligosaccharyl transferase subunit DAD1; defender against cell death 1
 Product Gene Name   

anti-DAD1 antibody

[Similar Products]
 Antibody/Peptide Pairs    DAD1 peptide (MBS152867) is used for blocking the activity of DAD1 antibody (MBS151036)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600243
 3D Structure    ModBase 3D Structure for P61803
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Purity/Purification    DAD1 Antibody is Ion exchange chromatography purified.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    DAD1 antibody was raised against a peptide corresponding to 14 amino acids near the C-terminus of human DAD1.
 Buffer    DAD1 Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    DAD1 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-DAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-DAD1 antibody

   DAD1 Antibody: Defender of cell death 1 (DAD1) was initially discovered in BHK21 cells as a negative regulator of programmed cell death, a process important for normal organism development and tissue homeostasis. DAD1 was later shown to be a subunit of the mammalian oligosaccharyltransferase complex and is required for its function and structural integrity. Mice lacking DAD1 express abnormal N-linked glycoproteins and undergo increased apoptotic-associated embryonic death. Furthermore, overexpression of DAD1 mRNA is seen in some human hepatocellular carcinomas, indicating it may also play a role in carcinogenesis. It should be noted that DAD1 is not related to the inhibitor of apoptosis proteins (IAP) family and does not contain any baculoviral IAP repeat (BIR) domains.
 Applications Tested/Suitable for anti-DAD1 antibody   

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-DAD1 antibody    DAD1 antibody can be used for detection of DAD1 by Western blot at 0.5 to 2 mug/mL. Despite its predicted molecular weight, DAD1 migrates at ~22 kDa in SDS-PAGE. Antibody can also be used for immunocytochemistry starting at 10 mug/mL. For immunofluorescence start at 10 mug/mL.
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 Western Blot (WB) of anti-DAD1 antibody    Western blot analysis of DAD1 in HepG2 cell lysate with DAD1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
anti-DAD1 antibody Western Blot (WB) (WB) image
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 Immunocytochemistry (ICC) of anti-DAD1 antibody    Immunocytochemistry of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
anti-DAD1 antibody Immunocytochemistry (ICC) (ICC) image
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 Immunofluorescence (IF) of anti-DAD1 antibody    Immunofluorescence of DAD1 in HepG2 cells with DAD1 antibody at 10 μg/mL.
anti-DAD1 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for DAD1. It may not necessarily be applicable to this product.
 NCBI GI #    14602573
 NCBI GeneID    1603
 NCBI Accession #    AAH09798 [Other Products]
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 UniProt Primary Accession #    P61803 [Other Products]
 UniProt Secondary Accession #    O08552; O70364; P46966; P46968; Q6FGA3; Q96GB7; D3DS25 [Other Products]
 UniProt Related Accession #    P61803 [Other Products]
 Molecular Weight    12,497 Da
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 NCBI Official Full Name    Defender against cell death 1
 NCBI Official Synonym Full Names    defender against cell death 1
 NCBI Official Symbol    DAD1 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1; DAD-1; oligosaccharyltransferase 2 homolog; oligosaccharyl transferase subunit DAD1; oligosaccharyltransferase subunit 2 (non-catalytic)
 UniProt Protein Name    Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit DAD1
 UniProt Synonym Protein Names   
Defender against cell death 1; DAD-1
 Protein Family    DASH complex
 UniProt Gene Name    DAD1 [Similar Products]
 UniProt Synonym Gene Names    Oligosaccharyl transferase subunit DAD1; DAD-1 [Similar Products]
 UniProt Entry Name    DAD1_HUMAN
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 NCBI Summary for DAD1    DAD1, the defender against apoptotic cell death, was initially identified as a negative regulator of programmed cell death in the temperature sensitive tsBN7 cell line. The DAD1 protein disappeared in temperature-sensitive cells following a shift to the nonpermissive temperature, suggesting that loss of the DAD1 protein triggered apoptosis. DAD1 is believed to be a tightly associated subunit of oligosaccharyltransferase both in the intact membrane and in the purified enzyme, thus reflecting the essential nature of N-linked glycosylation in eukaryotes. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DAD1    DAD1: Component of the N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). Loss of the DAD1 protein triggers apoptosis. Belongs to the DAD/OST2 family.

Protein type: Endoplasmic reticulum; Transferase; Glycan Metabolism - N-glycan biosynthesis; Membrane protein, integral; Apoptosis; Membrane protein, multi-pass; EC

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: endoplasmic reticulum membrane; membrane; oligosaccharyl transferase complex; integral to membrane

Molecular Function: dolichyl-diphosphooligosaccharide-protein glycotransferase activity; oligosaccharyl transferase activity

Biological Process: response to drug; blastocyst development; cellular protein metabolic process; apoptosis; protein amino acid glycosylation; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; response to nutrient; negative regulation of apoptosis
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 Research Articles on DAD1    1. single-nucleotide polymorphisms in DAD1 gene is associated with neuroendocrine tumor.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DAD1 antibodyPathways associated with anti-DAD1 antibody
 Reference Product  PubMed Publications
 STT3B antibody  >2 publications with DAD1 and STT3B
 STT3A antibody  >2 publications with DAD1 and STT3A
 OSTC antibody  >1 publications with DAD1 and OSTC
 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway antibodies  Defective ALG1 Causes ALG1-CDG (CDG-1k) Pathway Diagram
 Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway antibodies  Defective ALG11 Causes ALG11-CDG (CDG-1p) Pathway Diagram
 Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway antibodies  Defective ALG12 Causes ALG12-CDG (CDG-1g) Pathway Diagram
 Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway antibodies  Defective ALG14 Causes Congenital Myasthenic Syndrome (ALG14-CMS) Pathway Diagram
 Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway antibodies  Defective ALG2 Causes ALG2-CDG (CDG-1i) Pathway Diagram
 Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway antibodies  Defective ALG3 Causes ALG3-CDG (CDG-1d) Pathway Diagram
 Defective ALG6 Causes ALG6-CDG (CDG-1c) Pathway antibodies  Defective ALG6 Causes ALG6-CDG (CDG-1c) Pathway Diagram
 Defective ALG8 Causes ALG8-CDG (CDG-1h) Pathway antibodies  Defective ALG8 Causes ALG8-CDG (CDG-1h) Pathway Diagram
 Defective ALG9 Causes ALG9-CDG (CDG-1l) Pathway antibodies  Defective ALG9 Causes ALG9-CDG (CDG-1l) Pathway Diagram
Diseases associated with anti-DAD1 antibodyOrgans/Tissues associated with anti-DAD1 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >8 publications with DAD1 and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with DAD1 and Adenocarcinoma
 Liver Diseases Antibodies  >3 publications with DAD1 and Liver Diseases
 Fetal Death Antibodies  >3 publications with DAD1 and Fetal Death
 Inflammation Antibodies  >3 publications with DAD1 and Inflammation
 Liver Neoplasms Antibodies  >3 publications with DAD1 and Liver Neoplasms
 Immune System Diseases Antibodies  >2 publications with DAD1 and Immune System Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with DAD1 and Carcinoma, Hepatocellular
 Breast Neoplasms Antibodies  >1 publications with DAD1 and Breast Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with DAD1 and Cell Transformation, Neoplastic
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >8 publications with DAD1 and Blood
 Prostate Antibodies  >5 publications with DAD1 and Prostate
 Brain Antibodies  >5 publications with DAD1 and Brain
 Kidney Antibodies  >4 publications with DAD1 and Kidney
 Muscle Antibodies  >3 publications with DAD1 and Muscle
 Liver Antibodies  >3 publications with DAD1 and Liver
 Vascular Antibodies  >3 publications with DAD1 and Vascular
 Pancreas Antibodies  >3 publications with DAD1 and Pancreas
 Embryonic Tissue Antibodies  >2 publications with DAD1 and Embryonic Tissue
 Lung Antibodies  >2 publications with DAD1 and Lung
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