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anti-DAPK3 antibody :: Rabbit anti-Human, Mouse DAPK3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7047997
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DAPK3, Polyclonal Antibody

★Popular Item★
 Also Known As   

DAPK3 Antibody

 Product Synonym Names    Death-associated protein kinase 3; DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase; DAPK3; ZIPK
 Product Gene Name   

anti-DAPK3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    AB007144 mRNA
 3D Structure    ModBase 3D Structure for O43293
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Immunogen    Recombinant Human Death-associated protein kinase 3 protein (283-401AA)
 Preservative    0.03% Proclin 300
 Constituents    50% Glycerol, 0.01M PBS, PH 7.4
 Conjugation    Non-conjugated
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-8161 / sc-8162
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-DAPK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-DAPK3 antibody

   Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, translation and actin cytoskeleton reorganization. Involved in the regulation of smooth muscle contraction. Regulates both type I (caspase-dependent) apoptotic and type II (caspase-independent) autophagic cell deaths signal, depending on the cellular setting. Involved in regulation of starvation-induced autophagy. Regulates myosin phosphorylation in both smooth muscle and non-muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A; the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Phosphorylates MYL12B in non-muscle cells leading to reorganization of actin cytoskeleton. Isoform 2 can phosphorylate myosin, PPP1R12A and MYL12B. Overexpression leads to condensation of actin stress fibers into thick bundles. Involved in actin filament focal adhesion dynamics. The function in both reorganization of actin cytoskeleton and focal adhesion dissolution is modulated by RhoD. Positively regulates canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Phosphorylates RPL13A on 'Ser-77' upon interferon-gamma activation which is causing RPL13A release from the ribosome, RPL13A association with the GAIT complex and its subsequent involvement in transcript-selective translation inhibition. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Involved in regulation of cell cycle progression and cell proliferation. May be a tumor suppressor.
 Applications Tested/Suitable for anti-DAPK3 antibody   

ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)

 Application Notes for anti-DAPK3 antibody    WB: 1: 2000-1: 10000
IF: 1: 50-1: 200
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 Western Blot (WB) of anti-DAPK3 antibody    Western Blot
Positive WB detected in:HepG2 whole cell lysate,Mouse brain tissue
All lanes: DAPK3 antibody at 3.4ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 53,38 kDa
Observed band size: 53 kDa

anti-DAPK3 antibody Western Blot (WB) (WB) image
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 Immunofluorescence (IF) of anti-DAPK3 antibody    Immunofluorescent analysis of Hela cells using MBS7047997 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-DAPK3 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for DAPK3. It may not necessarily be applicable to this product.
 NCBI GI #    4557511
 NCBI GeneID    1613
 NCBI Accession #    NP_001339.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001348.2 [Other Products]
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 UniProt Primary Accession #    O43293 [Other Products]
 UniProt Secondary Accession #    Q05JY4; A0AVN4; B3KQE2 [Other Products]
 UniProt Related Accession #    O43293 [Other Products]
 Molecular Weight    37,048 Da
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 NCBI Official Full Name    death-associated protein kinase 3
 NCBI Official Synonym Full Names    death associated protein kinase 3
 NCBI Official Symbol    DAPK3 [Similar Products]
 NCBI Official Synonym Symbols   
DLK; ZIP; ZIPK
[Similar Products]
 NCBI Protein Information    death-associated protein kinase 3
 UniProt Protein Name    Death-associated protein kinase 3
 UniProt Synonym Protein Names   
DAP-like kinase; Dlk; MYPT1 kinase; Zipper-interacting protein kinase; ZIP-kinase
 Protein Family    Death-associated protein kinase
 UniProt Gene Name    DAPK3 [Similar Products]
 UniProt Synonym Gene Names    ZIPK; DAP kinase 3; Dlk; ZIP-kinase [Similar Products]
 UniProt Entry Name    DAPK3_HUMAN
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 NCBI Summary for DAPK3    Death-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DAPK3    DAPK3: Serine/threonine kinase which is involved in the regulation of apoptosis, autophagy, transcription, actin cytoskeleton reorganization, cell motility, smooth muscle contraction, and mitosis, particularly cytokinesis. Regulates both type I apoptotic and type II autophagic cell deaths signal, depending on the cellular setting. The former is caspase- dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Regulates myosin phosphorylation in both smooth muscle and non- muscle cells. In smooth muscle, regulates myosin either directly by phosphorylating MYL12B and MYL9 or through inhibition of smooth muscle myosin phosphatase (SMPP1M) via phosphorylation of PPP1R12A, and the inhibition of SMPP1M functions to enhance muscle responsiveness to Ca(2+) and promote a contractile state. Enhances transcription from AR-responsive promoters in a hormone- and kinase-dependent manner. Phosphorylates STAT3 and enhances its transcriptional activity. Positively regulates the canonical Wnt/beta-catenin signaling through interaction with NLK and TCF7L2. Can disrupt the NLK-TCF7L2 complex thereby influencing the phosphorylation of TCF7L2 by NLK. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection. Monomer and homotrimer. Can also exist as homodimer or form heterodimers with ATF4. Homodimerization is required for activation segment autophosphorylation Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. According to PubMed:17953487, does not interact with PARW. Interacts with AATF, CDC5L, UBE2D1, UBE2D2 AND UBE2D3. Interacts with AR and this interaction is enhanced by AATF. Interacts (via leucine zipper) with TCP10L (via leucine zipper). Interacts (via kinase domain) with DAPK1 (via kinase domain).Interacts with STAT3, NLK and TCF7L2. Isoform 1 and isoform 2 can interact with myosin and PPP1R12A. Isoform 2 is expressed in the bladder smooth muscle. Inhibited by pyridone 6 (K00225), a potent, ATP-competitive inhibitor. Phosphorylation at Thr-180, Thr-225 and Thr-265 is essential for activity. Oligomerization is required for full enzymatic activity. Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: CAMK group; DAPK family; EC 2.7.11.1; Kinase, protein; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Tumor suppressor

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; cAMP response element binding protein binding; identical protein binding; leucine zipper domain binding; protein binding; protein homodimerization activity; protein serine/threonine kinase activity; Rho GTPase binding

Biological Process: apoptosis; cytokinesis; negative regulation of translation; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of apoptosis; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of apoptosis; regulation of autophagy; regulation of focal adhesion formation; regulation of mitosis; regulation of mitotic cell cycle; regulation of smooth muscle contraction; regulation of transcription, DNA-dependent
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 Research Articles on DAPK3    1. The results revealed mutations caused a significant structural variation in DAPK3, concentrated in flexible loops forming part of the ATP binding pocket which have been associated with very low kinase activity, and the cellular progression towards cancer
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DAPK3 antibodyPathways associated with anti-DAPK3 antibody
 Reference Product  PubMed Publications
 MDM2 antibody  >2 publications with DAPK3 and MDM2
 DAXX antibody  >2 publications with DAPK3 and DAXX
 PPP1R12A antibody  >1 publications with DAPK3 and PPP1R12A
 PAWR antibody  >1 publications with DAPK3 and PAWR
 ATF4 antibody  >1 publications with DAPK3 and ATF4
 Products by Pathway  Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Caspase Activation Via Extrinsic Apoptotic Signalling Pathway antibodies  Caspase Activation Via Extrinsic Apoptotic Signalling Pathway Diagram
 Ligand-independent Caspase Activation Via DCC Pathway antibodies  Ligand-independent Caspase Activation Via DCC Pathway Diagram
 Pathways In Cancer antibodies  Pathways In Cancer Diagram
 Programmed Cell Death Pathway antibodies  Programmed Cell Death Pathway Diagram
Diseases associated with anti-DAPK3 antibodyOrgans/Tissues associated with anti-DAPK3 antibody
 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >7 publications with DAPK3 and Cardiovascular Diseases
 Inflammation Antibodies  >7 publications with DAPK3 and Inflammation
 Hypertension Antibodies  >6 publications with DAPK3 and Hypertension
 Carcinoma Antibodies  >4 publications with DAPK3 and Carcinoma
 Heart Diseases Antibodies  >3 publications with DAPK3 and Heart Diseases
 Lung Neoplasms Antibodies  >2 publications with DAPK3 and Lung Neoplasms
 Breast Neoplasms Antibodies  >2 publications with DAPK3 and Breast Neoplasms
 Hyperplasia Antibodies  >2 publications with DAPK3 and Hyperplasia
 Nervous System Diseases Antibodies  >2 publications with DAPK3 and Nervous System Diseases
 Neoplasms, Experimental Antibodies  >2 publications with DAPK3 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >15 publications with DAPK3 and Muscle
 Vascular Antibodies  >11 publications with DAPK3 and Vascular
 Blood Antibodies  >8 publications with DAPK3 and Blood
 Heart Antibodies  >6 publications with DAPK3 and Heart
 Lung Antibodies  >3 publications with DAPK3 and Lung
 Lymph Node Antibodies  >2 publications with DAPK3 and Lymph Node
 Prostate Antibodies  >2 publications with DAPK3 and Prostate
 Kidney Antibodies  >2 publications with DAPK3 and Kidney
 Bone Antibodies  >2 publications with DAPK3 and Bone
 Brain Antibodies  >2 publications with DAPK3 and Brain
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