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anti-DDAH1 antibody :: Goat DDAH1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421230
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DDAH1, Polyclonal Antibody

 Also Known As   

Goat anti-DDAH1 Antibody

 Product Synonym Names    DDAH1; dimethylarginine dimethylaminohydrolase 1; DDAH; DDAHI; NG, NG-dimethylarginine dimethylaminohydrolase; RP4-621F18.1; FLJ21264; FLJ25539; DDAH1 antibody; dimethylarginine dimethylaminohydrolase 1 antibody; DDAH antibody; DDAHI antibody; NG; NG-dimethylarginine dimethylaminohydrolase antibody; RP4-621F18.1 antibody; FLJ21264 antibody; FLJ25539 antibody
 Product Gene Name   

anti-DDAH1 antibody

[Similar Products]
 Antibody/Peptide Pairs    DDAH1 peptide (MBS425437) is used for blocking the activity of DDAH1 antibody (MBS421230)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    TCCSVLINKK VDS
 OMIM    604743
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Cow
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence TCCSVLINKKVDS, from the C Terminus of the protein sequence according to NP_036269.1; NP_001127917.1.
 Epitope    C Terminus
 Note    This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-DDAH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-DDAH1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-DDAH1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J.2005 Jul;19(9): 1175-7; PMID: 15827267). Recommended concentration: 0. 03-0.1ug/ml.
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 Western Blot (WB) of anti-DDAH1 antibody    (0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-DDAH1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for DDAH1. It may not necessarily be applicable to this product.
 NCBI GI #    6912328
 NCBI GeneID    23576
 NCBI Accession #    NP_036269.1 [Other Products]
 NCBI Related Accession #    NP_001127917.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_012137.3 [Other Products]
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 UniProt Secondary Accession #    Q5HYC8; Q86XK5 [Other Products]
 UniProt Related Accession #    O94760 [Other Products]
 Molecular Weight    20,189 Da
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 NCBI Official Full Name    N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 1
 NCBI Official Synonym Full Names    dimethylarginine dimethylaminohydrolase 1
 NCBI Official Symbol    DDAH1 [Similar Products]
 NCBI Official Synonym Symbols   
DDAH; HEL-S-16
[Similar Products]
 NCBI Protein Information    N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
 UniProt Protein Name    N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
 UniProt Synonym Protein Names   
DDAHI; Dimethylargininase-1
 Protein Family    N(G),N(G)-dimethylarginine dimethylaminohydrolase
 UniProt Gene Name    DDAH1 [Similar Products]
 UniProt Synonym Gene Names    DDAH; DDAH-1; Dimethylarginine dimethylaminohydrolase 1 [Similar Products]
 UniProt Entry Name    DDAH1_HUMAN
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 NCBI Summary for DDAH1    This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DDAH1    DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.3.18; Hydrolase

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: cytosol; mitochondrion

Molecular Function: amino acid binding; catalytic activity; dimethylargininase activity; metal ion binding

Biological Process: arginine catabolic process; citrulline metabolic process; nitric oxide mediated signal transduction; positive regulation of angiogenesis; positive regulation of nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure
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 Research Articles on DDAH1    1. Inhibiting the expression of DDAH1, but not DDAH2, resulted in a significant increase in the sensitivity of the EVT cell line SGHPL-4 to tumour necrosis factor related apoptosis inducing ligand (TRAIL) induced apoptosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-DDAH1 antibodyDiseases associated with anti-DDAH1 antibody
 Products by Pathway  Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Nitric Oxide Pathway antibodies  Metabolism Of Nitric Oxide Pathway Diagram
 ENOS Activation Pathway antibodies  ENOS Activation Pathway Diagram
 ENOS Activation And Regulation Pathway antibodies  ENOS Activation And Regulation Pathway Diagram
 Disease Name  Pubmed Publications
 Hypertension Antibodies  >21 publications with DDAH1 and Hypertension
 Disease Models, Animal Antibodies  >13 publications with DDAH1 and Disease Models, Animal
 Brain Diseases Antibodies  >10 publications with DDAH1 and Brain Diseases
 Fibrosis Antibodies  >9 publications with DDAH1 and Fibrosis
 Kidney Diseases Antibodies  >8 publications with DDAH1 and Kidney Diseases
 Inflammation Antibodies  >7 publications with DDAH1 and Inflammation
 Immune System Diseases Antibodies  >4 publications with DDAH1 and Immune System Diseases
 Necrosis Antibodies  >4 publications with DDAH1 and Necrosis
 Fatty Liver Antibodies  >3 publications with DDAH1 and Fatty Liver
 Hypertrophy Antibodies  >3 publications with DDAH1 and Hypertrophy
Organs/Tissues associated with anti-DDAH1 antibody
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >43 publications with DDAH1 and Vascular
 Heart Antibodies  >32 publications with DDAH1 and Heart
 Lung Antibodies  >17 publications with DDAH1 and Lung
 Kidney Antibodies  >17 publications with DDAH1 and Kidney
 Brain Antibodies  >15 publications with DDAH1 and Brain
 Muscle Antibodies  >14 publications with DDAH1 and Muscle
 Liver Antibodies  >13 publications with DDAH1 and Liver
 Embryonic Tissue Antibodies  >5 publications with DDAH1 and Embryonic Tissue
 Nerve Antibodies  >4 publications with DDAH1 and Nerve
 Prostate Antibodies  >3 publications with DDAH1 and Prostate
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