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| Product Name
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DDAH1, Polyclonal Antibody
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| Also Known As
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Goat anti-DDAH1 Antibody
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| Product Synonym Names
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DDAH1; dimethylarginine dimethylaminohydrolase 1; DDAH; DDAHI; NG, NG-dimethylarginine dimethylaminohydrolase; RP4-621F18.1; FLJ21264; FLJ25539; DDAH1 antibody; dimethylarginine dimethylaminohydrolase 1 antibody; DDAH antibody; DDAHI antibody; NG; NG-dimethylarginine dimethylaminohydrolase antibody; RP4-621F18.1 antibody; FLJ21264 antibody; FLJ25539 antibody
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| Product Gene Name
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[Similar Products]
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| Antibody/Peptide Pairs
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DDAH1 peptide (MBS425437) is used for blocking the activity of DDAH1 antibody (MBS421230)
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| Research Use Only
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For Research Use Only. Not for use in diagnostic procedures.
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 TOP
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| Immunogen Sequence
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TCCSVLINKK VDS
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| OMIM
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604743
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| Clonality
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Polyclonal
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| Host
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Goat
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| Species Reactivity
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Tested: Human; Expected from sequence similarity: Human, Cow
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| Purity/Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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| Form/Format
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Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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| Concentration
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100ug specific antibody in 200ul (lot specific)
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| Immunogen
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Peptide with sequence TCCSVLINKKVDS, from the C Terminus of the protein sequence according to NP_036269.1; NP_001127917.1.
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| Epitope
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C Terminus
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| Note
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This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
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| Preparation and Storage
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Aliquot and store at -20 degree C. Minimize freezing and thawing.
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| ISO Certification
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Manufactured in an ISO 9001:2015 Certified Laboratory.
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| Other Notes
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Small volumes of anti-DDAH1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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| Applications Tested/Suitable for anti-DDAH1 antibody
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Peptide ELISA (EIA), Western Blot (WB)
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| Application Notes for anti-DDAH1 antibody
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Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 35kDa band observed in Human Kidney lysates (calculated MW of 31.1kDa according to NP_036269.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Pullamsetti et al, FASEB J.2005 Jul;19(9): 1175-7; PMID: 15827267). Recommended concentration: 0. 03-0.1ug/ml.
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| Western Blot (WB) of anti-DDAH1 antibody
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(0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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| NCBI/Uniprot data below describe general gene information for DDAH1. It may not necessarily be applicable to this product. |
| NCBI GI #
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6912328
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| NCBI GeneID
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23576
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| NCBI Accession #
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NP_036269.1
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[Other Products]
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| NCBI Related Accession #
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NP_001127917.1
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[Other Products]
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| NCBI GenBank Nucleotide #
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NM_012137.3
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[Other Products]
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| UniProt Secondary Accession #
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Q5HYC8; Q86XK5
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[Other Products]
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| UniProt Related Accession #
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O94760
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[Other Products]
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| Molecular Weight
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20,189 Da
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TOP
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| NCBI Official Full Name
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N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 1
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| NCBI Official Synonym Full Names
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dimethylarginine dimethylaminohydrolase 1
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| NCBI Official Symbol
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DDAH1
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[Similar Products]
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| NCBI Official Synonym Symbols
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DDAH; HEL-S-16
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[Similar Products]
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| NCBI Protein Information
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N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
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| UniProt Protein Name
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N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
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| UniProt Synonym Protein Names
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DDAHI; Dimethylargininase-1
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| Protein Family
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N(G),N(G)-dimethylarginine dimethylaminohydrolase
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| UniProt Gene Name
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DDAH1
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[Similar Products]
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| UniProt Synonym Gene Names
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DDAH; DDAH-1; Dimethylarginine dimethylaminohydrolase 1
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[Similar Products]
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| UniProt Entry Name
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DDAH1_HUMAN
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| NCBI Summary for DDAH1
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This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]
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| UniProt Comments for DDAH1
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DDAH1: Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. Belongs to the DDAH family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.5.3.18; Hydrolase
Chromosomal Location of Human Ortholog: 1p22
Cellular Component: cytosol; mitochondrion
Molecular Function: amino acid binding; catalytic activity; dimethylargininase activity; metal ion binding
Biological Process: arginine catabolic process; citrulline metabolic process; nitric oxide mediated signal transduction; positive regulation of angiogenesis; positive regulation of nitric oxide biosynthetic process; regulation of nitric-oxide synthase activity; regulation of systemic arterial blood pressure
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| Research Articles on DDAH1
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1. Inhibiting the expression of DDAH1, but not DDAH2, resulted in a significant increase in the sensitivity of the EVT cell line SGHPL-4 to tumour necrosis factor related apoptosis inducing ligand (TRAIL) induced apoptosis
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| Precautions
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All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
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| Disclaimer
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While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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| Pathways associated with anti-DDAH1 antibody | Diseases associated with anti-DDAH1 antibody |
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| Organs/Tissues associated with anti-DDAH1 antibody |
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Antibody 
