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anti-DDB1 antibody :: Rabbit DDB1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS127654
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DDB1, Polyclonal Antibody

 Also Known As   

DDB1 Polyclonal Antibody

 Product Synonym Names    XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
 Product Gene Name   

anti-DDB1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    600045
 3D Structure    ModBase 3D Structure for Q16531
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
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 Species    Human
 Route    Synthetic Peptide
 Immunogen    A synthetic peptide of human DDB1
 Calculated Molecular Weight    127kDa
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-DDB1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-DDB1 antibody

   The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins.
 Product Categories/Family for anti-DDB1 antibody    Polyclonal
 Applications Tested/Suitable for anti-DDB1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-DDB1 antibody    WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
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 Western Blot (WB) of anti-DDB1 antibody    Western blot analysis of extracts of various cell lines, using DDB1 Antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
anti-DDB1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded human uterine cancer using DDB1 Antibody at dilution of 1:200 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded mouse testis using DDB1 Antibody at dilution of 1:200 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded human liver injury using DDB1 Antibody at dilution of 1:200 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded human kidney cancer using DDB1 Antibody at dilution of 1:200 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded human gastric cancer using DDB1 Antibody at dilution of 1:200 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded rat testis using DDB1 Antibody at dilution of 1:100 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded rat brain using DDB1 Antibody at dilution of 1:100 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-DDB1 antibody    Immunohistochemistry of paraffin-embedded human lung cancer using DDB1 Antibody at dilution of 1:100 (40x lens).
anti-DDB1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for DDB1. It may not necessarily be applicable to this product.
 NCBI GI #    12643730
 NCBI GeneID    1642
 NCBI Accession #    Q16531.1 [Other Products]
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 UniProt Primary Accession #    Q16531 [Other Products]
 UniProt Secondary Accession #    O15176; Q13289; Q58F96; A6NG77; B2R648; B4DG00 [Other Products]
 UniProt Related Accession #    Q16531 [Other Products]
 Molecular Weight    1140
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 NCBI Official Full Name    DNA damage-binding protein 1
 NCBI Official Synonym Full Names    damage-specific DNA binding protein 1, 127kDa
 NCBI Official Symbol    DDB1 [Similar Products]
 NCBI Official Synonym Symbols   
XPE; DDBA; XAP1; XPCE; XPE-BF; UV-DDB1
[Similar Products]
 NCBI Protein Information    DNA damage-binding protein 1; XAP-1; UV-DDB 1; DDB p127 subunit; XPE-binding factor; HBV X-associated protein 1; DNA damage-binding protein a; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; xeroderma pigmentosum group E-complementing protein
 UniProt Protein Name    DNA damage-binding protein 1
 UniProt Synonym Protein Names   
DDB p127 subunit; DNA damage-binding protein a; DDBa; Damage-specific DNA-binding protein 1; HBV X-associated protein 1; XAP-1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XPE-binding factor; XPE-BF; Xeroderma pigmentosum group E-complementing protein; XPCe
 Protein Family    DNA damage-binding protein
 UniProt Gene Name    DDB1 [Similar Products]
 UniProt Synonym Gene Names    XAP1; DDBa; XAP-1; UV-DDB 1; XPE-BF; XPCe [Similar Products]
 UniProt Entry Name    DDB1_HUMAN
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 NCBI Summary for DDB1    The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48) forms the small subunit. This protein complex functions in nucleotide-excision repair and binds to DNA following UV damage. Defective activity of this complex causes the repair defect in patients with xeroderma pigmentosum complementation group E (XPE) - an autosomal recessive disorder characterized by photosensitivity and early onset of carcinomas. However, it remains for mutation analysis to demonstrate whether the defect in XPE patients is in this gene or the gene encoding the small subunit. In addition, Best vitelliform mascular dystrophy is mapped to the same region as this gene on 11q, but no sequence alternations of this gene are demonstrated in Best disease patients. The protein encoded by this gene also functions as an adaptor molecule for the cullin 4 (CUL4) ubiquitin E3 ligase complex by facilitating the binding of substrates to this complex and the ubiquitination of proteins. [provided by RefSeq, May 2012]
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 UniProt Comments for DDB1    DDB1: the large subunit of theUV- damaged DNA-binding protein complex (the UV-DDB complex) required for DNA repair. The UV- DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as a component of numerous distinct DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. The functional specificity of the DCX E3 ubiquitin- protein ligase complex is determined by the variable substrate recognition component recruited by DDB1. DCX(DDB2) (also known as DDB1-CUL4-ROC1, CUL4-DDB-ROC1 and CUL4-DDB-RBX1) may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. DCX(DDB2) also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of p53 in response to radiation-induced DNA damage and during DNA replication. DCX(ERCC8) (the CSA complex) plays a role in transcription-coupled repair (TCR). May also play a role in ubiquitination of p27kip when associated with CUL4 and SKP2. Component of the UV-DDB complex which includes DDB1 and DDB2. The UV-DDB complex interacts with monoubiquitinated histone H2A and binds to XPC via the DDB2 subunit. Component of numerous DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes which consist of a core of DDB1, CUL4A or CUL4B and RBX1. DDB1 may recruit specific substrate targeting subunits to the DCX complex. These substrate targeting subunits are generally known as DCAF (DDB1- and CUL4-associated factor) or CDW (CUL4-DDB1-associated WD40-repeat) proteins. Interacts with AMBRA1, ATG16L1, BTRC, DCAF1, DCAF17, DCAF16, DCAF15, DDA1, DET1, DTL, ERCC8, FBXW5, FBXW8, GRWD1, DCAF6, KATNB1, NLE1, NUP43, PAFAH1B1, PHIP, PWP1, RBBP4, RBBP5, RBBP7, RFWD2, SNRNP40, VPRBP, WDR5, WDR5B, WDR12, DCAF4, DCAF5, DCAF11, WDR26, DCAF10, WDR39, DCAF12, WDR42, DCAF8, WDR53, WDR59, WDR61, DCAF7, WSB1, WSB2 and WDTC1. DCX complexes may associate with the COP9 signalosome, and this inhibits the E3 ubiquitin-protein ligase activity of the complex. Interacts with NF2, TSC1 and TSC2. Interacts with Simian virus 5 protein V and the HBV X protein. Interaction with SV5 protein V may prevent the recruitment of DCAF proteins to DCX complexes. Interacts with EIF2C1 and EIF2C2. Associates with the E3 ligase complex containing DYRK2, UBR5, DDB1 and VPRBP proteins (EDVP complex). Interacts directly with DYRK2. Belongs to the DDB1 family.

Protein type: DNA repair, damage

Chromosomal Location of Human Ortholog: 11q12-q13

Cellular Component: nucleoplasm; extracellular space; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; damaged DNA binding

Biological Process: proteasomal ubiquitin-dependent protein catabolic process; positive regulation of viral genome replication; Wnt receptor signaling pathway; viral reproduction; nucleotide-excision repair; protein ubiquitination during ubiquitin-dependent protein catabolic process; nucleotide-excision repair, DNA damage removal; positive regulation of viral protein levels in host cell; interaction with symbiont; DNA repair; negative regulation of apoptosis
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 Research Articles on DDB1    1. The study presents the crystal structure of human CRBN bound to DDB1 and the drug lenalidomide.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DDB1 antibodyPathways associated with anti-DDB1 antibody
 Reference Product  PubMed Publications
 CUL4A antibody  >107 publications with DDB1 and CUL4A
 DDB2 antibody  >81 publications with DDB1 and DDB2
 RBX1 antibody  >34 publications with DDB1 and RBX1
 CUL4B antibody  >14 publications with DDB1 and CUL4B
 DTL antibody  >13 publications with DDB1 and DTL
 CRBN antibody  >9 publications with DDB1 and CRBN
 ERCC8 antibody  >5 publications with DDB1 and ERCC8
 FBXW5 antibody  >2 publications with DDB1 and FBXW5
 TRPC4AP antibody  >1 publications with DDB1 and TRPC4AP
 Products by Pathway  Pathway Diagram
 Cul4-DDB1-CSA Complex Pathway antibodies  Cul4-DDB1-CSA Complex Pathway Diagram
 Cul4-DDB1-CSA Complex Pathway antibodies  Cul4-DDB1-CSA Complex Pathway Diagram
 Cul4-DDB1-DDB2 Complex Pathway antibodies  Cul4-DDB1-DDB2 Complex Pathway Diagram
 Cul4-DDB1-DDB2 Complex Pathway antibodies  Cul4-DDB1-DDB2 Complex Pathway Diagram
 DNA Repair Pathway antibodies  DNA Repair Pathway Diagram
 Dual Incision Reaction In GG-NER Pathway antibodies  Dual Incision Reaction In GG-NER Pathway Diagram
 Formation Of Incision Complex In GG-NER Pathway antibodies  Formation Of Incision Complex In GG-NER Pathway Diagram
 Global Genomic NER (GG-NER) Pathway antibodies  Global Genomic NER (GG-NER) Pathway Diagram
 Hepatitis B Pathway antibodies  Hepatitis B Pathway Diagram
 Nucleotide Excision Repair Pathway antibodies  Nucleotide Excision Repair Pathway Diagram
Diseases associated with anti-DDB1 antibodyOrgans/Tissues associated with anti-DDB1 antibody
 Disease Name  Pubmed Publications
 Hepatitis Antibodies  >25 publications with DDB1 and Hepatitis
 Liver Diseases Antibodies  >10 publications with DDB1 and Liver Diseases
 Nervous System Diseases Antibodies  >7 publications with DDB1 and Nervous System Diseases
 Liver Neoplasms Antibodies  >5 publications with DDB1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >5 publications with DDB1 and Carcinoma, Hepatocellular
 Neoplasms, Experimental Antibodies  >3 publications with DDB1 and Neoplasms, Experimental
 Ovarian Diseases Antibodies  >3 publications with DDB1 and Ovarian Diseases
 Abnormalities, Drug-Induced Antibodies  >3 publications with DDB1 and Abnormalities, Drug-Induced
 Anemia Antibodies  >3 publications with DDB1 and Anemia
 Necrosis Antibodies  >3 publications with DDB1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >17 publications with DDB1 and Blood
 Liver Antibodies  >17 publications with DDB1 and Liver
 Skin Antibodies  >16 publications with DDB1 and Skin
 Brain Antibodies  >8 publications with DDB1 and Brain
 Embryonic Tissue Antibodies  >5 publications with DDB1 and Embryonic Tissue
 Kidney Antibodies  >4 publications with DDB1 and Kidney
 Eye Antibodies  >4 publications with DDB1 and Eye
 Lung Antibodies  >4 publications with DDB1 and Lung
 Nerve Antibodies  >4 publications with DDB1 and Nerve
 Muscle Antibodies  >4 publications with DDB1 and Muscle
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