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anti-DDT antibody :: Rabbit anti-Human DDT Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS175215
Western Blot (WB)
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DDT, Polyclonal Antibody

 Also Known As   

Anti-human DDT antibody

 Product Synonym Names    D-dopachrome decarboxylase; D-dopachrome tautomerase; D dopachrome decarboxylase antibody; D dopachrome tautomerase antibody; D-dopachrome decarboxylase antibody; D-dopachrome tautomerase antibody; DDCT antibody; Ddt antibody; Phenylpyruvate tautomerase II antibody
 Product Gene Name   

anti-DDT antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602750
 3D Structure    ModBase 3D Structure for P30046
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized
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 Immunogen    E Coli-derived human DDT recombinant protein (Position: M1-L118).
 Contents    Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
 Reconstitution    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-DDT antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-DDT antibody

   Description: Rabbit IgG polyclonal antibody for D-dopachrome decarboxylase(DDT) detection. Tested with WB, IHC-P, ICC, ELISA in Human.
Background: DDT, D-dopachrome tautomerization, converts D-dopachrome into 5,6-dihydroxyindole. Northern blot analysis revealed that DDT was expressed as a 0.6-kb mRNA in all tissues tested, with the strongest expression in liver. The DDT gene in human and mouse is identical in exon structure to the MIF gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure.the genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10.
 Applications Tested/Suitable for anti-DDT antibody   

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), ELISA (EIA)

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 Western Blot (WB) of anti-DDT antibody    Figure. Western blot analysis of DDT using anti- DDT antibody (MBS175215).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane: Recombinant Human DDT Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- DDT antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DDT at approximately 15KD. The expected band size for DDT is at 15KD.
anti-DDT antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for DDT. It may not necessarily be applicable to this product.
 NCBI GI #    2828192
 NCBI GeneID    1652
 NCBI Accession #    P30046.3 [Other Products]
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 UniProt Primary Accession #    P30046 [Other Products]
 UniProt Secondary Accession #    O00774; O60787; Q13534; B7Z522 [Other Products]
 UniProt Related Accession #    P30046 [Other Products]
 Molecular Weight    11,323 Da
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 NCBI Official Full Name    D-dopachrome decarboxylase
 NCBI Official Synonym Full Names    D-dopachrome tautomerase
 NCBI Official Symbol    DDT [Similar Products]
 NCBI Official Synonym Symbols   
DDCT
[Similar Products]
 NCBI Protein Information    D-dopachrome decarboxylase; phenylpyruvate tautomerase II
 UniProt Protein Name    D-dopachrome decarboxylase
 UniProt Synonym Protein Names   
D-dopachrome tautomerase; Phenylpyruvate tautomerase II
 Protein Family    D-dopachrome decarboxylase
 UniProt Gene Name    DDT [Similar Products]
 UniProt Entry Name    DOPD_HUMAN
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 NCBI Summary for DDT    D-dopachrome tautomerase converts D-dopachrome into 5,6-dihydroxyindole. The DDT gene is related to the migration inhibitory factor (MIF) in terms of sequence, enzyme activity, and gene structure. DDT and MIF are closely linked on chromosome 22. [provided by RefSeq, Jul 2008]
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 UniProt Comments for DDT    DDT: Tautomerization of D-dopachrome with decarboxylation to give 5,6-dihydroxyindole (DHI). Belongs to the MIF family.

Protein type: EC 4.1.1.84; Lyase

Chromosomal Location of Human Ortholog: 22q11.23

Cellular Component: cytoplasm

Molecular Function: D-dopachrome decarboxylase activity; dopachrome isomerase activity

Biological Process: melanin biosynthetic process
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 Research Articles on DDT    1. findings identify DDT as a functionally redundant but more potent cytokine to MIF in cancer and suggest that current attempts to inhibit MIF signaling may fail because of DDT compensation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DDT antibodyDiseases associated with anti-DDT antibody
 Reference Product  PubMed Publications
 MIF antibody  >8 publications with DDT and MIF
 DPT antibody  >3 publications with DDT and DPT
 DCT antibody  >1 publications with DDT and DCT
 Disease Name  Pubmed Publications
 Poisoning Antibodies  >480 publications with DDT and Poisoning
 Liver Diseases Antibodies  >213 publications with DDT and Liver Diseases
 Liver Neoplasms Antibodies  >138 publications with DDT and Liver Neoplasms
 Disease Models, Animal Antibodies  >137 publications with DDT and Disease Models, Animal
 Death Antibodies  >133 publications with DDT and Death
 Brain Diseases Antibodies  >91 publications with DDT and Brain Diseases
 Immune System Diseases Antibodies  >84 publications with DDT and Immune System Diseases
 Liver Neoplasms, Experimental Antibodies  >80 publications with DDT and Liver Neoplasms, Experimental
 Congenital Abnormalities Antibodies  >70 publications with DDT and Congenital Abnormalities
 Prenatal Injuries Antibodies  >65 publications with DDT and Prenatal Injuries
Organs/Tissues associated with anti-DDT antibody
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >1262 publications with DDT and Liver
 Adipose Tissue Antibodies  >650 publications with DDT and Adipose Tissue
 Connective Tissue Antibodies  >615 publications with DDT and Connective Tissue
 Brain Antibodies  >420 publications with DDT and Brain
 Kidney Antibodies  >233 publications with DDT and Kidney
 Skin Antibodies  >135 publications with DDT and Skin
 Lung Antibodies  >109 publications with DDT and Lung
 Heart Antibodies  >108 publications with DDT and Heart
 Ovary Antibodies  >108 publications with DDT and Ovary
 Embryonic Tissue Antibodies  >101 publications with DDT and Embryonic Tissue
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