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anti-DEPDC5 antibody :: Rabbit DEPD5 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9207358
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DEPD5 (DEPDC5), Polyclonal Antibody

★Popular Item★
 Also Known As   

DEPD5 Antibody (C-term)

 Product Synonym Names    DEP domain-containing protein 5; DEPDC5; KIAA0645
 Product Gene Name   

anti-DEPDC5 antibody

[Similar Products]
 Antibody/Peptide Pairs    DEPD5 peptide (MBS9218794) is used for blocking the activity of DEPD5 antibody (MBS9207358)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    1404-1432
 OMIM    604364
 3D Structure    ModBase 3D Structure for O75140
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human (Predicted Reactivity: Mouse)
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 Specificity    This DEPD5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1404-1432 amino acids of human DEPD5.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.49 (lot specific)
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 Antigen Type    Synthetic Peptide
 Clone Names    RB31640
 Antigen Source    HUMAN
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-DEPDC5 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-DEPDC5 antibody    Cancer; Neuroscience
 Applications Tested/Suitable for anti-DEPDC5 antibody   

Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)

 Application Notes for anti-DEPDC5 antibody    FC~~1:25
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 Flow Cytometry of anti-DEPDC5 antibody    Overlay histogram showing Hela cells stained with MBS9207358 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (MBS9207358, 1:25 dilution) for 60 min at 37║C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight« 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37║C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
anti-DEPDC5 antibody Flow Cytometry (FC/FACS) image
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 Western Blot of anti-DEPDC5 antibody    All lanes : Anti-DEPD5 Antibody (C-term) at 1:2000 dilution Lane 1: HL-60 whole cell lysate Lane 2: KG-1 whole cell lysate Lane 3: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 171 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
anti-DEPDC5 antibody Western Blot (WB) image
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NCBI/Uniprot data below describe general gene information for DEPDC5. It may not necessarily be applicable to this product.
 NCBI GI #    55749924
 NCBI GeneID    9681
 NCBI Accession #    NP_001007189.1 [Other Products]
 NCBI Related Accession #    NP_001129501.1; NP_001229825.1; NP_001229826.1; NP_055477.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001007188.2 [Other Products]
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 UniProt Primary Accession #    O75140 [Other Products]
 UniProt Secondary Accession #    Q5K3V5; Q5THY9; Q5THZ0; Q5THZ1; Q5THZ3; Q68DR1; Q6MZX3; A6H8V6; A8MPX9; B4DH93; B9EGN9 [Other Products]
 UniProt Related Accession #    O75140 [Other Products]
 Molecular Weight    181264
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 NCBI Official Full Name    DEP domain-containing protein 5 isoform 2
 NCBI Official Synonym Full Names    DEP domain containing 5
 NCBI Official Symbol    DEPDC5 [Similar Products]
 NCBI Official Synonym Symbols   
[Similar Products]
 NCBI Protein Information    DEP domain-containing protein 5
 UniProt Protein Name    DEP domain-containing protein 5
 Protein Family    DEP domain-containing protein
 UniProt Gene Name    DEPDC5 [Similar Products]
 UniProt Synonym Gene Names    KIAA0645 [Similar Products]
 UniProt Entry Name    DEPD5_HUMAN
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 NCBI Summary for DEPDC5    This gene encodes a member of the IML1 family of proteins involved in G-protein signaling pathways. The mechanistic target of rapamycin complex 1 (mTORC1) pathway regulates cell growth by sensing the availability of nutrients. The protein encoded by this gene is a component of the GATOR1 (GAP activity toward Rags) complex which inhibits the amino acid-sensing branch of the mTORC1 pathway. Mutations in this gene are associated with autosomal dominant familial focal epilepsy with variable foci. A single nucleotide polymorphism in an intron of this gene has been associated with an increased risk of hepatocellular carcinoma in individuals with chronic hepatitis C virus infection. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]
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 UniProt Comments for DEPDC5    DEPDC5: Belongs to the IML1 family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 22q12.3

Cellular Component: perinuclear region of cytoplasm; lysosomal membrane; cytosol

Molecular Function: GTPase activator activity

Biological Process: positive regulation of GTPase activity

Disease: Epilepsy, Familial Focal, With Variable Foci
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Product References and Citations for anti-DEPDC5 antibody

   Seng, T.J., et al. Genes Chromosomes Cancer 43(2):181-193(2005)
Dunham, I., et al. Nature 402(6761):489-495(1999)
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 Research Articles on DEPDC5    1. An association was made for DEPDC5 with sporadic focal cortical dysplasia and also hemimegalencephaly.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Diseases associated with anti-DEPDC5 antibodyOrgans/Tissues associated with anti-DEPDC5 antibody
 Disease Name  Pubmed Publications
 Carcinoma, Hepatocellular Antibodies  >7 publications with DEPDC5 and Carcinoma, Hepatocellular
 Epilepsy, Partial, with Variable Foci Antibodies  >6 publications with DEPDC5 and Epilepsy, Partial, with Variable Foci
 Liver Neoplasms Antibodies  >4 publications with DEPDC5 and Liver Neoplasms
 Nervous System Malformations Antibodies  >2 publications with DEPDC5 and Nervous System Malformations
 Congenital Abnormalities Antibodies  >2 publications with DEPDC5 and Congenital Abnormalities
 Fibrosis Antibodies  >1 publications with DEPDC5 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >12 publications with DEPDC5 and Brain
 Liver Antibodies  >6 publications with DEPDC5 and Liver
 Blood Antibodies  >1 publications with DEPDC5 and Blood
 Muscle Antibodies  >1 publications with DEPDC5 and Muscle
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