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anti-Dffb antibody :: Rabbit anti-Human DFF40 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS150231
Unit / Price
0.1 mg  /  $365 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DFF40 (Dffb), Polyclonal Antibody

★Popular Item★
 Also Known As   

DFF40 Antibody

 Product Synonym Names    DFF40; CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik; Cad; DNA fragmentation factor subunit beta; Caspase-activated deoxyribonuclease; CAD; DNA fragmentation factor, beta subunit
 Product Gene Name   

anti-Dffb antibody

[Similar Products]
 Antibody/Peptide Pairs    DFF40 peptide (MBS152764) is used for blocking the activity of DFF40 antibody (MBS150231)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for O54788
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    DFF40 Antibody is affinity chromatography purified via peptide column.
 Form/Format    Liquid
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 Conjugate    Unconjugated
 Immunogen    DFF40 antibody was raised against a 16 amino acid peptide near the amino terminus of human DFF40.
 Buffer    DFF40 Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    DFF40 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-Dffb antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-Dffb antibody

   DFF40 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD for caspase activated deoxyribonuclease. The human homologue of mouse CAD was more recently identified by three groups independently and termed CPAN, DFF40, and human CAD, respectively. DFF45/ICAD is the inhibitory protein of DFF40/CAD and forms complex with DFF40/CAD. Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of DFF40/CAD, which causes DNA degradation, is the hallmark of apoptotic cell death.
 Applications Tested/Suitable for anti-Dffb antibody   

ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)

 Application Notes for anti-Dffb antibody    DFF40 antibody can be used for detection of DFF40 by Western blot at 0.5 mug/mL. A 40 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.

User Note: Optimal dilutions for each application to be determined by the researcher.

Positive Control:
MBS151739 K562 Cell Lysate
MBS151729 Jurkat Cell Lysate
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 Western Blot (WB) of anti-Dffb antibody    Western blot analysis of DFF40 in Jurkat (J) and K562 (K) whole cell lysate with DFF40 antibody at 1:500 dilution.
anti-Dffb antibody Western Blot (WB) (WB) image
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 Immunocytochemistry (ICC) of anti-Dffb antibody    Immunocytochemistry of DFF40 in Jurkat cells with DFF40 antibody at 5 μg/mL.
anti-Dffb antibody Immunocytochemistry (ICC) (ICC) image
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 Immunofluorescence (IF) of anti-Dffb antibody    Immunofluorescence of DFF40 in Jurkat cells with DFF40 antibody at 20 μg/mL.
anti-Dffb antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for Dffb. It may not necessarily be applicable to this product.
 NCBI GI #    160948620
 NCBI GeneID    13368
 NCBI Accession #    NP_031885 [Other Products]
 NCBI GenBank Nucleotide #    NM_007859.4 [Other Products]
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 UniProt Primary Accession #    O54788 [Other Products]
 UniProt Related Accession #    O54788 [Other Products]
 Molecular Weight    40 kDa
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 NCBI Official Full Name    DNA fragmentation factor subunit beta
 NCBI Official Synonym Full Names    DNA fragmentation factor, beta subunit
 NCBI Official Symbol    Dffb [Similar Products]
 NCBI Official Synonym Symbols   
CAD; CPAN; 40kDa; DFF40; Didff; 5730477D02Rik
[Similar Products]
 NCBI Protein Information    DNA fragmentation factor subunit beta; DFF-40; caspase-activated DNase; caspase-activated deoxyribonuclease; DNA fragmentation factor 40 kDa subunit; DNase inhibited by DNA fragmentation factor; DNA fragmentation factor, 40 kD, beta subunit
 UniProt Protein Name    DNA fragmentation factor subunit beta
 UniProt Synonym Protein Names   
Caspase-activated deoxyribonuclease; CAD; Caspase-activated DNase; DNA fragmentation factor 40 kDa subunit; DFF-40
 Protein Family    DNA fragmentation factor
 UniProt Gene Name    Dffb [Similar Products]
 UniProt Synonym Gene Names    Cad; CAD; Caspase-activated DNase; DFF-40 [Similar Products]
 UniProt Entry Name    DFFB_MOUSE
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 UniProt Comments for Dffb    DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; EC 3.-.-.-; Deoxyribonuclease

Cellular Component: cytoplasm; nuclear chromatin; nucleolus; intracellular; nucleus; cytosol

Molecular Function: protein binding; nuclease activity; enzyme binding; DNA binding; hydrolase activity; deoxyribonuclease activity; GPI-anchor transamidase activity

Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation
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 Research Articles on Dffb    1. the highest order of chromatin compaction observed in the later steps of caspase-dependent apoptosis relies on DFF40/CAD-mediated DNA damage by generating 3'-OH ends in single-strand rather than double-strand DNA nicks/breaks
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-Dffb antibodyDiseases associated with anti-Dffb antibody
 Products by Pathway  Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptosis Signaling Pathway antibodies  Apoptosis Signaling Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 FAS Pathway And Stress Induction Of HSP Regulation antibodies  FAS Pathway And Stress Induction Of HSP Regulation Diagram
 Programmed Cell Death Pathway antibodies  Programmed Cell Death Pathway Diagram
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >13 publications with Dffb and Necrosis
 Carcinoma Antibodies  >7 publications with Dffb and Carcinoma
 Adenocarcinoma Antibodies  >4 publications with Dffb and Adenocarcinoma
 Immune System Diseases Antibodies  >4 publications with Dffb and Immune System Diseases
 Neoplasms, Experimental Antibodies  >4 publications with Dffb and Neoplasms, Experimental
 Cognition Disorders Antibodies  >3 publications with Dffb and Cognition Disorders
 Cardiovascular Diseases Antibodies  >3 publications with Dffb and Cardiovascular Diseases
 Inflammation Antibodies  >2 publications with Dffb and Inflammation
 Disease Models, Animal Antibodies  >2 publications with Dffb and Disease Models, Animal
 Lung Neoplasms Antibodies  >1 publications with Dffb and Lung Neoplasms
Organs/Tissues associated with anti-Dffb antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >9 publications with Dffb and Brain
 Lung Antibodies  >3 publications with Dffb and Lung
 Kidney Antibodies  >2 publications with Dffb and Kidney
 Spinal Cord Antibodies  >2 publications with Dffb and Spinal Cord
 Skin Antibodies  >1 publications with Dffb and Skin
 Testis Antibodies  >1 publications with Dffb and Testis
 Bone Marrow Antibodies  >1 publications with Dffb and Bone Marrow
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