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anti-DNMT1 antibody :: Goat DNMT1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421587
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

DNMT1, Polyclonal Antibody

★Popular Item★
 Also Known As   

Goat anti-DNMT1 Antibody

 Product Synonym Names    DNMT1; DNA (cytosine-5-)-methyltransferase 1; CXXC9; DNMT; FLJ16293; MCMT; MGC104992; DNA methyltransferase 1; DNMT1 antibody; DNA (cytosine-5-)-methyltransferase 1 antibody; CXXC9 antibody; DNMT antibody; FLJ16293 antibody; MCMT antibody; MGC104992 antibody; DNA methyltransferase 1 antibody
 Product Gene Name   

anti-DNMT1 antibody

[Similar Products]
 Antibody/Peptide Pairs    DNMT1 peptide (MBS426741) is used for blocking the activity of DNMT1 antibody (MBS421587)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    RFESPPKTQP TEDN
 OMIM    126375
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-RFESPPKTQPTEDN, from the internal egion of the protein sequence according to NP_001370.1.
 Epitope    Internal egion
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-DNMT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-DNMT1 antibody   

Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-DNMT1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Immunohistochemistry: In paraffin embedded Human Kidney shows nuclear staining in PCT. Recommended concentration, 3-6ug/ml.
Western Blot: Approx 170kDa band observed in lysates of cell line Jurkat (calculated MW of 183kDa according to NP_001370.1). Recommended concentration: 0.5-1.5ug/ml.
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 Western Blot (WB) of anti-DNMT1 antibody    (0.5ug/ml) staining of Jurkat cell lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-DNMT1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-DNMT1 antibody    (3.8ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
anti-DNMT1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for DNMT1. It may not necessarily be applicable to this product.
 NCBI GI #    4503351
 NCBI GeneID    1786
 NCBI Accession #    NP_001370.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001379.3 [Other Products]
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 UniProt Secondary Accession #    Q9UHG5; Q9ULA2; Q9UMZ6; A0AV63; B7ZLW6 [Other Products]
 UniProt Related Accession #    P26358 [Other Products]
 Molecular Weight    144,465 Da
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 NCBI Official Full Name    DNA (cytosine-5)-methyltransferase 1 isoform b
 NCBI Official Synonym Full Names    DNA (cytosine-5-)-methyltransferase 1
 NCBI Official Symbol    DNMT1 [Similar Products]
 NCBI Official Synonym Symbols   
AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI
[Similar Products]
 NCBI Protein Information    DNA (cytosine-5)-methyltransferase 1
 UniProt Protein Name    DNA (cytosine-5)-methyltransferase 1
 UniProt Synonym Protein Names   
CXXC-type zinc finger protein 9; DNA methyltransferase HsaI; DNA MTase HsaI; M.HsaI; MCMT
 UniProt Gene Name    DNMT1 [Similar Products]
 UniProt Synonym Gene Names    AIM; CXXC9; DNMT; Dnmt1; DNA MTase HsaI; M.HsaI [Similar Products]
 UniProt Entry Name    DNMT1_HUMAN
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 NCBI Summary for DNMT1    This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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 UniProt Comments for DNMT1    DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: EC 2.1.1.37; Methyltransferase, DNA; Cell development/differentiation; Transcription regulation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: cell soma; centric heterochromatin; cytoplasm; nucleoplasm; nucleus; protein complex; replication fork

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; DNA binding; DNA-methyltransferase activity; double-stranded DNA binding; estrogen receptor binding; histone deacetylase binding; methyl-CpG binding; protein binding; protein domain specific binding; RNA binding; unmethylated CpG binding; zinc ion binding

Biological Process: aging; brain development; chromatin modification; cytosine methylation within a CG sequence; DNA methylation; gene silencing; maintenance of DNA methylation; negative regulation of gene expression, epigenetic; negative regulation of histone H3-K9 methylation; negative regulation of transcription from RNA polymerase II promoter; neuron differentiation; positive regulation of histone H3-K4 methylation; Ras protein signal transduction; regulation of cell proliferation; response to activity; response to caffeine; response to estradiol stimulus; response to ethanol; response to heat; response to ionizing radiation; response to lipopolysaccharide; response to testosterone stimulus; response to toxin; response to vitamin A; S-adenosylhomocysteine metabolic process; S-adenosylmethioninamine metabolic process; S-adenosylmethionine metabolic process; transcription, DNA-dependent

Disease: Cerebellar Ataxia, Deafness, And Narcolepsy, Autosomal Dominant; Neuropathy, Hereditary Sensory, Type Ie
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 Research Articles on DNMT1    1. These studies reveal that USP7-mediated stabilization of DNMT1 is regulated by acetylation and provide a structural basis for the design of inhibitors.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-DNMT1 antibodyPathways associated with anti-DNMT1 antibody
 Reference Product  PubMed Publications
 DNMT3A antibody  >687 publications with DNMT1 and DNMT3A
 DNMT3B antibody  >650 publications with DNMT1 and DNMT3B
 HDAC1 antibody  >138 publications with DNMT1 and HDAC1
 PCNA antibody  >74 publications with DNMT1 and PCNA
 EZH2 antibody  >54 publications with DNMT1 and EZH2
 HDAC2 antibody  >53 publications with DNMT1 and HDAC2
 EHMT2 antibody  >14 publications with DNMT1 and EHMT2
 USP7 antibody  >9 publications with DNMT1 and USP7
 SMARCA5 antibody  >5 publications with DNMT1 and SMARCA5
 Products by Pathway  Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 Cysteine And Methionine Metabolism Pathway antibodies  Cysteine And Methionine Metabolism Pathway Diagram
 DNA Methylation Pathway antibodies  DNA Methylation Pathway Diagram
 Epigenetic Regulation Of Gene Expression Pathway antibodies  Epigenetic Regulation Of Gene Expression Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 Methionine Degradation Pathway antibodies  Methionine Degradation Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram
Diseases associated with anti-DNMT1 antibodyOrgans/Tissues associated with anti-DNMT1 antibody
 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >263 publications with DNMT1 and Carcinoma
 Adenocarcinoma Antibodies  >124 publications with DNMT1 and Adenocarcinoma
 Breast Neoplasms Antibodies  >112 publications with DNMT1 and Breast Neoplasms
 Liver Diseases Antibodies  >77 publications with DNMT1 and Liver Diseases
 Disease Models, Animal Antibodies  >75 publications with DNMT1 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >74 publications with DNMT1 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >70 publications with DNMT1 and Lung Neoplasms
 Carcinoma, Hepatocellular Antibodies  >69 publications with DNMT1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >66 publications with DNMT1 and Liver Neoplasms
 Neoplasms, Experimental Antibodies  >47 publications with DNMT1 and Neoplasms, Experimental
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >224 publications with DNMT1 and Blood
 Brain Antibodies  >169 publications with DNMT1 and Brain
 Liver Antibodies  >146 publications with DNMT1 and Liver
 Lung Antibodies  >120 publications with DNMT1 and Lung
 Embryonic Tissue Antibodies  >58 publications with DNMT1 and Embryonic Tissue
 Prostate Antibodies  >57 publications with DNMT1 and Prostate
 Stomach Antibodies  >48 publications with DNMT1 and Stomach
 Muscle Antibodies  >48 publications with DNMT1 and Muscle
 Bone Antibodies  >42 publications with DNMT1 and Bone
 Kidney Antibodies  >42 publications with DNMT1 and Kidney
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