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anti-EEF2 antibody :: Rabbit EEF2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS8222746
Unit / Price
0.03 mL  /  $165 +1 FREE 8GB USB
0.1 mL  /  $245 +1 FREE 8GB USB
0.2 mL  /  $350 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EEF2, Polyclonal Antibody

 Also Known As   

Anti-EEF2 (pT56) Antibody

 Product Synonym Names    EF2; Elongation factor 2; EF-2
 Product Gene Name   

anti-EEF2 antibody

[Similar Products]
 Antibody/Peptide Pairs    EEF2 peptide (MBS8225409) is used for blocking the activity of EEF2 antibody (MBS8222746)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    130610
 3D Structure    ModBase 3D Structure for P13639
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Bovine, Chicken, Monkey, Zebrafish
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 Specificity    Recognizes endogenous levels of EEF2 (pT56) protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EEF2 (pT56). The exact sequence is proprietary.
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-EEF2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-EEF2 antibody

   Rabbit polyclonal antibody to EEF2 (pT56)
 Applications Tested/Suitable for anti-EEF2 antibody   

Western Blot (WB),Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)

 Application Notes for anti-EEF2 antibody    WB (1/500 - 1/1000), IHC (1/100 - 1/200), IF (1/100 - 1/500), ICC (1/100 - 1/500)
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 Western Blot (WB) of anti-EEF2 antibody    Western blot analysis of EEF2 (pT56) expression in SKOV3 (A), NIH3T3 (B) whole cell lysates.
anti-EEF2 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-EEF2 antibody    Immunohistochemical analysis of EEF2 (pT56) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-EEF2 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-EEF2 antibody    Immunofluorescent analysis of EEF2 (pT56) staining in SKOV3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-EEF2 antibody Immunofluorescence (IF) image
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NCBI/Uniprot data below describe general gene information for EEF2. It may not necessarily be applicable to this product.
 NCBI GI #    4503483
 NCBI GeneID    1938
 NCBI Accession #    NP_001952.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001961.3 [Other Products]
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 UniProt Primary Accession #    P13639 [Other Products]
 UniProt Secondary Accession #    Q58J86; B2RMP5; D6W618 [Other Products]
 UniProt Related Accession #    P13639 [Other Products]
 Molecular Weight    95,338 Da
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 NCBI Official Full Name    elongation factor 2
 NCBI Official Synonym Full Names    eukaryotic translation elongation factor 2
 NCBI Official Symbol    EEF2 [Similar Products]
 NCBI Official Synonym Symbols   
EF2; EF-2; EEF-2; SCA26
[Similar Products]
 NCBI Protein Information    elongation factor 2
 UniProt Protein Name    Elongation factor 2
 Protein Family    Elongation factor
 UniProt Gene Name    EEF2 [Similar Products]
 UniProt Synonym Gene Names    EF2; EF-2 [Similar Products]
 UniProt Entry Name    EF2_HUMAN
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 NCBI Summary for EEF2    This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]
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 UniProt Comments for EEF2    EEF2: a member of the GTP-binding translation elongation factor family. An essential factor for protein synthesis. Promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by eEF2 kinase phosphorylation. eEF2 kinase is normally dependent on Ca2+ ions and calmodulin. eEF2 kinase can also be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. A variety of treatments known to raise intracellular Ca2+ or cAMP levels have been shown to result in increased phosphorylation of eEF2, and thus to inhibit peptide-chain elongation.

Protein type: Translation elongation; Translation

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: polysome; membrane; cytoplasm; plasma membrane; cytosol; nucleus; ribonucleoprotein complex

Molecular Function: GTPase activity; protein binding; GTP binding; protein kinase binding; translation elongation factor activity

Biological Process: cellular protein metabolic process; translational elongation; positive regulation of translation; translation; peptidyl-diphthamide biosynthetic process from peptidyl-histidine; hemopoietic progenitor cell differentiation; pathogenesis; gene expression; post-translational protein modification

Disease: Spinocerebellar Ataxia 26
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 Research Articles on EEF2    1. nuclear localization of active eEF-2 depends upon its interaction with p53, as cells lacking p53 contain less active eEF-2 in the nuclear compartment
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EEF2 antibodyPathways associated with anti-EEF2 antibody
 Reference Product  PubMed Publications
 RPS6 antibody  >12 publications with EEF2 and RPS6
 RPS23 antibody  >1 publications with EEF2 and RPS23
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Disease Pathway antibodies  Disease Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway antibodies  Gamma Carboxylation, Hypusine Formation And Arylsulfatase Activation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Infectious Disease Pathway antibodies  Infectious Disease Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
Diseases associated with anti-EEF2 antibodyOrgans/Tissues associated with anti-EEF2 antibody
 Disease Name  Pubmed Publications
 Hypertrophy Antibodies  >23 publications with EEF2 and Hypertrophy
 Carcinoma Antibodies  >16 publications with EEF2 and Carcinoma
 Cardiovascular Diseases Antibodies  >12 publications with EEF2 and Cardiovascular Diseases
 Adenocarcinoma Antibodies  >10 publications with EEF2 and Adenocarcinoma
 Disease Models, Animal Antibodies  >9 publications with EEF2 and Disease Models, Animal
 Necrosis Antibodies  >8 publications with EEF2 and Necrosis
 Heart Diseases Antibodies  >8 publications with EEF2 and Heart Diseases
 Breast Neoplasms Antibodies  >8 publications with EEF2 and Breast Neoplasms
 Inflammation Antibodies  >6 publications with EEF2 and Inflammation
 Liver Diseases Antibodies  >5 publications with EEF2 and Liver Diseases
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >102 publications with EEF2 and Muscle
 Brain Antibodies  >45 publications with EEF2 and Brain
 Blood Antibodies  >39 publications with EEF2 and Blood
 Heart Antibodies  >27 publications with EEF2 and Heart
 Liver Antibodies  >20 publications with EEF2 and Liver
 Kidney Antibodies  >12 publications with EEF2 and Kidney
 Vascular Antibodies  >10 publications with EEF2 and Vascular
 Lung Antibodies  >8 publications with EEF2 and Lung
 Pancreas Antibodies  >7 publications with EEF2 and Pancreas
 Bone Antibodies  >5 publications with EEF2 and Bone
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