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anti-EGF antibody :: Rabbit anti-Mouse, Rat EGF R Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS221529 anti-EGF antibody
Unit / Price
0.05 mg  /  $500 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EGF R, Polyclonal Antibody

 Also Known As   

RABBIT ANTI HUMAN EGF RECEPTOR

 Product Gene Name   

anti-EGF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    131550
 3D Structure    ModBase 3D Structure for P00533
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse, Rat
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 Form/Format    Purified
Purified IgG - liquid
 Concentration    IgG concentration 1.0mg/ml (lot specific)
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 Perservative Stabilisers    0.01% Sodium Azide (NaN3)
 Buffer Solution    Phosphate buffered saline
 Antiserum Preparation    Antisera to EGF receptor were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
 Target Species    Human
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 Preparation and Storage    Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
 Other Notes    Small volumes of anti-EGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-EGF antibody

   Rabbit anti Human epidermal growth factor receptor antibody recognizes human epidermal growth factor receptor (EGFR), also known as Proto-oncogene c-ErbB-1. EGFR is a 1210 amino acid single pass type 1 ~170kDa transmembrane glycoprotein which is a member of the ErbB family of receptor tyrosine kinases. EGFR is the cell surface receptor for members of the EGF family, and functions in early embryonic development and in the renewal of stem cells in tissues such as the skin, liver and gut (Nautiyal et al. 2010). Mutations to EGFR have been found in many human carcinomas including breast, brain, lung, and colon(Garcia de Palazzo et al. 1993).
 Applications Tested/Suitable for anti-EGF antibody   

ELISA (EIA), Western Blot (WB)

 Application Notes for anti-EGF antibody    Western Blot: This item detects a band of approximately 170kDa in A431 cell lysates.
ELISA: Minimum Dilution: 1/8000; Maximum Dilution: 1/32000
Western Blotting: Minimum Dilution: 1/500; Maximum Dilution: 1/5000
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NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.
 NCBI GI #    29725609
 NCBI GeneID    1956
 NCBI Accession #    NP_005219.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_005228.3 [Other Products]
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 UniProt Primary Accession #    P00533 [Other Products]
 UniProt Secondary Accession #    O00688; O00732; P06268; Q14225; Q68GS5; Q92795; Q9BZS2; Q9GZX1; Q9H2C9; Q9H3C9; Q9UMD7 [Other Products]
 UniProt Related Accession #    P00533 [Other Products]
 Molecular Weight    69,228 Da
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 NCBI Official Full Name    epidermal growth factor receptor isoform a
 NCBI Official Synonym Full Names    epidermal growth factor receptor
 NCBI Official Symbol    EGFR [Similar Products]
 NCBI Official Synonym Symbols   
ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
[Similar Products]
 NCBI Protein Information    epidermal growth factor receptor; avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; erb-b2 receptor tyrosine kinase 1; proto-oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1
 UniProt Protein Name    Epidermal growth factor receptor
 UniProt Synonym Protein Names   
Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1
 Protein Family    Pro-epidermal growth factor
 UniProt Gene Name    EGFR [Similar Products]
 UniProt Synonym Gene Names    ERBB; ERBB1; HER1 [Similar Products]
 UniProt Entry Name    EGFR_HUMAN
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 NCBI Summary for EGF    The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. Multiple alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2010]
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 UniProt Comments for EGF    EGFR: a receptor tyrosine kinase. This is a receptor for epidermal growth factor (EGF) and related growth factors including TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30, and vaccinia virus growth factor. EGFR is involved in the control of cell growth and differentiation. It is a single-pass transmembrane tyrosine kinase. Ligand binding to this receptor results in receptor dimerization, autophosphorylation (in trans), activation of various downstream signaling molecules and lysosomal degradation. It can be phosphorylated and activated by Src. Activated EGFR binds the SH2 domain of phospholipase C-gamma (PLC-gamma), activating PLC-gamma-mediated downstream signaling. Phosphorylated EGFR binds Cbl, leading to its ubiquitination and degradation. Grb2 and SHC bind to phospho-EGFR and are involved in the activation of MAP kinase signaling pathways. Phosphorylation on Ser and Thr residues is thought to represent a mechanism for attenuation of EGFR kinase activity. EGFR is overexpressed in breast, head and neck cancers, correlating with poor survival. Activating somatic mutations are seen in lung cancer, corresponding to the minority of patients with strong responses to the EGFR inhibitor Iressa (gefitinib). Mutations and amplifications are also seen in glioblastoma, and upregulation is seen in colon cancer and neoplasms. In xenografts, inhibitors synergize with cytotoxic drugs in the inhibition of many tumor types. Inhibitors include: Iressa/ZD1839, Erbitux, Tarceva, and lapatinib. Four alternatively spliced isoforms have been described.

Protein type: Membrane protein, integral; Kinase, protein; Protein kinase, TK; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Tumor suppressor; TK group; EGFR family

Chromosomal Location of Human Ortholog: 7p12

Cellular Component: extracellular space; endoplasmic reticulum membrane; nuclear membrane; focal adhesion; cell surface; basolateral plasma membrane; integral to membrane; lipid raft; Golgi membrane; membrane; perinuclear region of cytoplasm; cytoplasm; apical plasma membrane; plasma membrane; AP-2 adaptor complex; endosome membrane; nucleus; endosome; receptor complex

Molecular Function: identical protein binding; epidermal growth factor receptor activity; epidermal growth factor binding; nitric-oxide synthase regulator activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; protein phosphatase binding; protein kinase binding; actin filament binding; integrin binding; protein binding; enzyme binding; transmembrane receptor activity; MAP kinase kinase kinase activity; protein heterodimerization activity; ubiquitin protein ligase binding; protein-tyrosine kinase activity; double-stranded DNA binding; chromatin binding; glycoprotein binding; ATP binding

Biological Process: circadian rhythm; diterpenoid metabolic process; positive regulation of nitric oxide biosynthetic process; activation of MAPKK activity; nerve growth factor receptor signaling pathway; alkanesulfonate metabolic process; positive regulation of vasodilation; protein insertion into membrane; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; positive regulation of MAP kinase activity; positive regulation of fibroblast proliferation; cell-cell adhesion; cell surface receptor linked signal transduction; ovulation cycle; hair follicle development; positive regulation of superoxide release; negative regulation of mitotic cell cycle; fibroblast growth factor receptor signaling pathway; positive regulation of DNA repair; digestive tract morphogenesis; response to osmotic stress; phospholipase C activation; hydrogen peroxide metabolic process; response to hydroxyisoflavone; positive regulation of transcription from RNA polymerase II promoter; regulation of nitric-oxide synthase activity; response to oxidative stress; response to calcium ion; negative regulation of protein catabolic process; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of epidermal growth factor receptor signaling pathway; tongue development; axon guidance; embryonic placenta development; peptidyl-tyrosine phosphorylation; translation; protein amino acid autophosphorylation; positive regulation of smooth muscle cell proliferation; signal transduction; positive regulation of synaptic transmission, glutamatergic; learning and/or memory; positive regulation of cell proliferation; salivary gland morphogenesis; response to stress; regulation of peptidyl-tyrosine phosphorylation; epidermal growth factor receptor signaling pathway; ossification; phosphoinositide-mediated signaling; MAPKKK cascade; liver development; positive regulation of protein kinase B signaling cascade; cell proliferation; cerebral cortex cell migration; calcium-dependent phospholipase A2 activation; positive regulation of vasoconstriction; innate immune response; positive regulation of protein amino acid phosphorylation; astrocyte activation; response to cobalamin; positive regulation of DNA replication; positive regulation of phosphorylation; lung development; positive regulation of cell migration; positive regulation of inflammatory response

Disease: Lung Cancer
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 Research Articles on EGF    1. We observed EGFR alteration in a subset of cases with operable state gastric cancer and determined that it was associated with an unfavorable prognosis
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-EGF antibodyDiseases associated with anti-EGF antibody
 Products by Pathway  Pathway Diagram
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 Disease Name  Pubmed Publications
 Carcinoma Antibodies  >9487 publications with EGF and Carcinoma
 Lung Neoplasms Antibodies  >5270 publications with EGF and Lung Neoplasms
 Adenocarcinoma Antibodies  >3959 publications with EGF and Adenocarcinoma
 Kidney Diseases Antibodies  >3902 publications with EGF and Kidney Diseases
 Small Cell Lung Carcinoma Antibodies  >3542 publications with EGF and Small Cell Lung Carcinoma
 Carcinoma, Non-Small-Cell Lung Antibodies  >3501 publications with EGF and Carcinoma, Non-Small-Cell Lung
 Disease Progression Antibodies  >3006 publications with EGF and Disease Progression
 Breast Neoplasms Antibodies  >2643 publications with EGF and Breast Neoplasms
 Carcinoma, Squamous Cell Antibodies  >2552 publications with EGF and Carcinoma, Squamous Cell
 Head and Neck Neoplasms Antibodies  >2008 publications with EGF and Head and Neck Neoplasms
Organs/Tissues associated with anti-EGF antibody
 Organ/Tissue Name  Pubmed Publications
 Lung Antibodies  >7147 publications with EGF and Lung
 Kidney Antibodies  >5399 publications with EGF and Kidney
 Vascular Antibodies  >2462 publications with EGF and Vascular
 Brain Antibodies  >2073 publications with EGF and Brain
 Liver Antibodies  >1279 publications with EGF and Liver
 Skin Antibodies  >1207 publications with EGF and Skin
 Heart Antibodies  >1187 publications with EGF and Heart
 Muscle Antibodies  >674 publications with EGF and Muscle
 Prostate Antibodies  >659 publications with EGF and Prostate
 Bone Antibodies  >519 publications with EGF and Bone
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