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anti-EIF2S1 antibody :: Rabbit EIF2A Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS125750
Western Blot (WB)
Unit / Price
0.05 mL  /  $230 +1 FREE 8GB USB
0.1 mL  /  $305 +1 FREE 8GB USB
0.2 mL  /  $460 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EIF2A (EIF2S1), Polyclonal Antibody

★Popular Item★
 Also Known As   

EIF2A Polyclonal Antibody

 Product Synonym Names    eIF2alpha: eIF2a; EIF2S1; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A
 Product Gene Name   

anti-EIF2S1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    603907
 3D Structure    ModBase 3D Structure for P05198
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Purity/Purification    Affinity Purification
 Concentration    1mg/ml (lot specific)
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 Species    Human
 Immunogen    Recombinant Protein
 Immunogen    Fusion protein of human EIF2A
 Calculated Molecular Weight    36kDa
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 Preparation and Storage    Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
 Other Notes    Small volumes of anti-EIF2S1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-EIF2S1 antibody

   Phosphorylation of the eukaryotic initiation factor 2 (eIF2) alpha subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. eIF2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the alpha subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-gamma and TNF-alpha induces potent phosphorylation of eIF2alpha at Ser51 (5,6).
 Product Categories/Family for anti-EIF2S1 antibody    Polyclonal
 Applications Tested/Suitable for anti-EIF2S1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-EIF2S1 antibody    WB: 1:500 - 1:1000
IHC: 1:50 - 1:100
IF: 1:20 - 1:50
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 Western Blot (WB) of anti-EIF2S1 antibody    Western blot analysis of extracts of various cell lines, using EIF2S1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
anti-EIF2S1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for EIF2S1. It may not necessarily be applicable to this product.
 NCBI GI #    124200
 NCBI GeneID    1965
 NCBI Accession #    P05198.3 [Other Products]
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 UniProt Primary Accession #    P05198 [Other Products]
 UniProt Related Accession #    P05198 [Other Products]
 Molecular Weight    315
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 NCBI Official Full Name    Eukaryotic translation initiation factor 2 subunit 1
 NCBI Official Synonym Full Names    eukaryotic translation initiation factor 2, subunit 1 alpha, 35kDa
 NCBI Official Symbol    EIF2S1 [Similar Products]
 NCBI Official Synonym Symbols   
EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
[Similar Products]
 NCBI Protein Information    eukaryotic translation initiation factor 2 subunit 1; eIF-2-alpha; eukaryotic translation initiation factor 2 subunit alpha
 UniProt Protein Name    Eukaryotic translation initiation factor 2 subunit 1
 UniProt Synonym Protein Names   
Eukaryotic translation initiation factor 2 subunit alpha; eIF-2-alpha; eIF-2A; eIF-2alpha
 Protein Family    Eukaryotic translation initiation factor
 UniProt Gene Name    EIF2S1 [Similar Products]
 UniProt Synonym Gene Names    EIF2A; eIF-2-alpha; eIF-2A; eIF-2alpha [Similar Products]
 UniProt Entry Name    IF2A_HUMAN
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 NCBI Summary for EIF2S1    The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
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 UniProt Comments for EIF2S1    eIF2-alpha: a translation initiation factor that functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40s ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit to form the 80S initiation complex is preceded by hydrolysis of the GTP bound to eIF-2 and release of an eIF-2-GDP binary complex. In order for eIF-2 to recycle and catalyze another round of initiation, the GDP bound to eIF-2 must exchange with GTP by way of a reaction catalyzed by eIF-2B. Phosphorylated by at least 4 kinases: PERK, GCN2, HRI and PKR. Phosphorylation stabilizes the eIF-2/GDP/eIF-2B complex and prevents GDP/GTP exchange reaction, thus impairing the recycling of eIF-2 between successive rounds of initiation and leading to global inhibition of translation. Upregulated in some thyroid cancers and bronchiolo-alveolar adenocarcinomas; aberrant phosphorylation correlates with Alzheimer disease and Epstein-Barr virus infections.

Protein type: RNA-binding; Translation initiation; Translation

Chromosomal Location of Human Ortholog: 14q23.3

Cellular Component: eukaryotic translation initiation factor 2B complex; polysome; membrane; stress granule; nucleus; cytosol; eukaryotic translation initiation factor 2 complex

Molecular Function: protein binding; translation initiation factor activity; ribosome binding

Biological Process: regulation of translation initiation in response to stress; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; translation; unfolded protein response; protein amino acid autophosphorylation; translational initiation; gene expression
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 Research Articles on EIF2S1    1. This review addresses on the past and current efforts to delineate the molecular mechanisms of eIF2alpha in tumorigenesis, tumor progression, resistance to therapy, and tumor cachexia
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-EIF2S1 antibodyDiseases associated with anti-EIF2S1 antibody
 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway antibodies  Formation Of The Ternary Complex, And Subsequently, The 43S Complex Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Hepatitis C Pathway antibodies  Hepatitis C Pathway Diagram
 Herpes Simplex Infection Pathway antibodies  Herpes Simplex Infection Pathway Diagram
 Disease Name  Pubmed Publications
 Death Antibodies  >4 publications with EIF2S1 and Death
 Brain Diseases Antibodies  >1 publications with EIF2S1 and Brain Diseases
 Cell Transformation, Neoplastic Antibodies  >1 publications with EIF2S1 and Cell Transformation, Neoplastic
 Hyperglycemia Antibodies  >1 publications with EIF2S1 and Hyperglycemia
 Glucose Intolerance Antibodies  >1 publications with EIF2S1 and Glucose Intolerance
 Disease Models, Animal Antibodies  >1 publications with EIF2S1 and Disease Models, Animal
 Inflammation Antibodies  >1 publications with EIF2S1 and Inflammation
Organs/Tissues associated with anti-EIF2S1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >2 publications with EIF2S1 and Brain
 Pancreas Antibodies  >1 publications with EIF2S1 and Pancreas
 Blood Antibodies  >1 publications with EIF2S1 and Blood
 Placenta Antibodies  >1 publications with EIF2S1 and Placenta
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