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anti-EIF4A3 antibody :: Rabbit anti-Human EIF4A3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7048081
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EIF4A3, Polyclonal Antibody

★Popular Item★
 Also Known As   

EIF4A3 Antibody

 Product Synonym Names    Eukaryotic initiation factor 4A-III; ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic initiation facto; EIF4A3; DDX48; KIAA0111
 Product Gene Name   

anti-EIF4A3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    X79538 mRNA
 3D Structure    ModBase 3D Structure for P38919
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Immunogen    Recombinant human Eukaryotic initiation factor 4A-III protein (6-166AA)
 Preservative    0.03% Proclin 300
 Constituents    50% Glycerol, 0.01M PBS, PH 7.4
 Conjugation    Non-conjugated
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-51487 / sc-67369
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-EIF4A3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-EIF4A3 antibody

   ATP-dependent RNA helicase. Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH-RBM8A heterodimer, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH-RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S); the function is different from the established EJC assembly. Involved in craniofacial development.
 Applications Tested/Suitable for anti-EIF4A3 antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-EIF4A3 antibody    WB: 1: 1000-1: 5000
IHC: 1: 20-1: 200
IF: 1: 50-1: 200
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 Western Blot (WB) of anti-EIF4A3 antibody    Western Blot
Positive WB detected in:A549 whole cell lysate,MCF-7 whole cell lysate
All lanes: EIF4A3 antibody at 3ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 47 kDa
Observed band size: 47 kDa

anti-EIF4A3 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-EIF4A3 antibody    Immunohistochemistry of paraffin-embedded human gastric cancer using MBS7048081 at dilution of 1:100
anti-EIF4A3 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-EIF4A3 antibody    Immunofluorescent analysis of MCF-7 cells using MBS7048081 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-EIF4A3 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for EIF4A3. It may not necessarily be applicable to this product.
 NCBI GI #    7661920
 NCBI GeneID    9775
 NCBI Accession #    NP_055555.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_014740.3 [Other Products]
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 UniProt Primary Accession #    P38919 [Other Products]
 UniProt Secondary Accession #    Q15033; Q6IBQ2; Q96A18 [Other Products]
 UniProt Related Accession #    P38919 [Other Products]
 Molecular Weight    46,871 Da
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 NCBI Official Full Name    eukaryotic initiation factor 4A-III
 NCBI Official Synonym Full Names    eukaryotic translation initiation factor 4A3
 NCBI Official Symbol    EIF4A3 [Similar Products]
 NCBI Official Synonym Symbols   
RCPS; DDX48; MUK34; NUK34; NMP265; eIF4AIII
[Similar Products]
 NCBI Protein Information    eukaryotic initiation factor 4A-III
 UniProt Protein Name    Eukaryotic initiation factor 4A-III
 UniProt Synonym Protein Names   
ATP-dependent RNA helicase DDX48; ATP-dependent RNA helicase eIF4A-3; DEAD box protein 48; Eukaryotic initiation factor 4A-like NUK-34; Eukaryotic translation initiation factor 4A isoform 3; Nuclear matrix protein 265; NMP 265; hNMP 265
 Protein Family    Eukaryotic initiation factor
 UniProt Gene Name    EIF4A3 [Similar Products]
 UniProt Synonym Gene Names    DDX48; KIAA0111; eIF-4A-III; eIF4A-III; NMP 265; hNMP 265 [Similar Products]
 UniProt Entry Name    IF4A3_HUMAN
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 NCBI Summary for EIF4A3    This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]
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 UniProt Comments for EIF4A3    DDX48: ATP-dependent RNA helicase. Component of a splicing- dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC, that remains bound to spliced mRNAs throughout all stages of mRNA metabolism, functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). Constitutes at least part of the platform anchoring other EJC proteins to spliced mRNAs. Its RNA-dependent ATPase and RNA-helicase activities are induced by CASC3, but abolished in presence of the MAGOH/RBM8A heterodimer, thereby trapping the ATP- bound EJC core onto spliced mRNA in a stable conformation. The inhibition of ATPase activity by the MAGOH/RBM8A heterodimer increases the RNA-binding affinity of the EJC. Involved in translational enhancement of spliced mRNAs after formation of the 80S ribosome complex. Binds spliced mRNA in sequence-independent manner, 20-24 nucleotides upstream of mRNA exon-exon junctions. Shows higher affinity for single-stranded RNA in an ATP-bound core EJC complex than after the ATP is hydrolyzed. Belongs to the DEAD box helicase family. eIF4A subfamily.

Protein type: EC 3.6.4.13; Helicase; RNA splicing; RNA-binding; Spliceosome

Chromosomal Location of Human Ortholog: 17q25.3

Cellular Component: cytoplasm; cytosol; membrane; nucleoplasm

Molecular Function: ATP binding; ATP-dependent RNA helicase activity; mRNA binding; poly(A) binding; protein binding

Biological Process: embryonic cranial skeleton morphogenesis; mRNA 3'-end processing; mRNA catabolic process, nonsense-mediated decay; mRNA export from nucleus; negative regulation of translation; nuclear mRNA splicing, via spliceosome; poly(A) tail shortening; positive regulation of translation; RNA export from nucleus; RNA secondary structure unwinding; RNA splicing; termination of RNA polymerase II transcription

Disease: Robin Sequence With Cleft Mandible And Limb Anomalies
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 Research Articles on EIF4A3    1. Host eIF4AIII RNA helicase is required for efficient human cytomegalovirus replication.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EIF4A3 antibodyPathways associated with anti-EIF4A3 antibody
 Reference Product  PubMed Publications
 MAGOH antibody  >15 publications with EIF4A3 and MAGOH
 UPF1 antibody  >6 publications with EIF4A3 and UPF1
 UPF3B antibody  >6 publications with EIF4A3 and UPF3B
 CASC3 antibody  >5 publications with EIF4A3 and CASC3
 RNPS1 antibody  >4 publications with EIF4A3 and RNPS1
 CWC22 antibody  >3 publications with EIF4A3 and CWC22
 UPF3A antibody  >2 publications with EIF4A3 and UPF3A
 Products by Pathway  Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 Cleavage Of Growing Transcript In The Termination Region Pathway antibodies  Cleavage Of Growing Transcript In The Termination Region Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Exon Junction Complex (EJC) Pathway antibodies  Exon Junction Complex (EJC) Pathway Diagram
 Exon Junction Complex (EJC) Pathway antibodies  Exon Junction Complex (EJC) Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 ISG15 Antiviral Mechanism Pathway antibodies  ISG15 Antiviral Mechanism Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
Diseases associated with anti-EIF4A3 antibodyOrgans/Tissues associated with anti-EIF4A3 antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >4 publications with EIF4A3 and Nervous System Diseases
 Craniofacial Abnormalities Antibodies  >2 publications with EIF4A3 and Craniofacial Abnormalities
 Neoplasm Metastasis Antibodies  >1 publications with EIF4A3 and Neoplasm Metastasis
 Liver Diseases Antibodies  >1 publications with EIF4A3 and Liver Diseases
 Carcinoma, Hepatocellular Antibodies  >1 publications with EIF4A3 and Carcinoma, Hepatocellular
 Neurodegenerative Diseases Antibodies  >1 publications with EIF4A3 and Neurodegenerative Diseases
 Liver Neoplasms Antibodies  >1 publications with EIF4A3 and Liver Neoplasms
 Heart Diseases Antibodies  >1 publications with EIF4A3 and Heart Diseases
 Learning Disorders Antibodies  >1 publications with EIF4A3 and Learning Disorders
 Memory Disorders Antibodies  >1 publications with EIF4A3 and Memory Disorders
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >8 publications with EIF4A3 and Brain
 Blood Antibodies  >5 publications with EIF4A3 and Blood
 Muscle Antibodies  >3 publications with EIF4A3 and Muscle
 Heart Antibodies  >2 publications with EIF4A3 and Heart
 Bone Antibodies  >2 publications with EIF4A3 and Bone
 Liver Antibodies  >2 publications with EIF4A3 and Liver
 Embryonic Tissue Antibodies  >2 publications with EIF4A3 and Embryonic Tissue
 Nerve Antibodies  >2 publications with EIF4A3 and Nerve
 Lung Antibodies  >2 publications with EIF4A3 and Lung
 Connective Tissue Antibodies  >1 publications with EIF4A3 and Connective Tissue
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