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anti-EP300 antibody :: Rabbit anti-Human EP300 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7046078
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EP300, Polyclonal Antibody

★Popular Item★
 Also Known As   

EP300 Antibody

 Product Synonym Names    Histone acetyltransferase p300; E1A-associated protein p300; EP300; P300
 Product Gene Name   

anti-EP300 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    602700
 3D Structure    ModBase 3D Structure for Q09472
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    >95%, Protein G purified
 Form/Format    Liquid
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 Species    Human
 Immunogen    Recombinant human Histone acetyltransferase p300 protein
 Conjugate    Non-conjugated
 Buffer    Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-585 / sc-8981 / sc-584
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-EP300 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-EP300 antibody

   Functions as histone acetyltransferase and regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Histone acetylation gives an epigenetic tag for transcriptional activation. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Mediates acetylation of histone H3 at 'Lys-122' (H3K122ac), a modification that localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability. Mediates acetylation of histone H3 at 'Lys-27' (H3K27ac). Also functions as acetyltransferase for nonhistone targets. Acetylates 'Lys-131' of ALX1 and acts as its coactivator. Acetylates SIRT2 and is proposed to indirectly increase the transcriptional activity of TP53 through acetylation and subsequent attenuation of SIRT2 deacetylase function. Acetylates HDAC1 leading to its inactivation and modulation of transcription. Acts as a TFAP2A-mediated transcriptional coactivator in presence of CITED2. Plays a role as a coactivator of NEUROD1-dependent transcription of the secretin and p21 genes and controls terminal differentiation of cells in the intestinal epithelium. Promotes cardiac myocyte enlargement. Can also mediate transcriptional repression. Binds to and may be involved in the transforming capacity of the adenovirus E1A protein. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes. Acetylates FOXO1 and enhances its transcriptional activity. Acetylates BCL6 wich disrupts its ability to recruit histone deacetylases and hinders its transcriptional repressor activity. Participates in CLOCK or NPAS2-regulated rhythmic gene transcription; exhibits a circadian association with CLOCK or NPAS2, correlating with increase in PER1/2 mRNA and histone H3 acetylation on the PER1/2 promoter. Acetylates MTA1 at 'Lys-626' which is essential for its transcriptional coactivator activity (PubMed:10733570, PubMed:11430825, PubMed:11701890, PubMed:12402037, PubMed:12586840, PubMed:12929931, PubMed:14645221, PubMed:15186775, PubMed:15890677, PubMed:16617102, PubMed:16762839, PubMed:18722353, PubMed:18995842, PubMed:23415232, PubMed:23911289, PubMed:23934153, PubMed:8945521). Acetylates XBP1 isoform 2; acetylation increases protein stability of XBP1 isoform 2 and enhances its transcriptional activity (PubMed:20955178). Acetylates PCNA; acetylation promotes removal of chromatin-bound PCNA and its degradation during nucleotide excision repair (NER) (PubMed:24939902). Acetylates MEF2D.
 Applications Tested/Suitable for anti-EP300 antibody   

ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF)

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 Immunohistochemistry (IHC) of anti-EP300 antibody    Immunohistochemistry of paraffin-embedded MBS7046078 using human bladder cancer at dilution 1:100
anti-EP300 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-EP300 antibody    Immunohistochemistry of paraffin-embedded human skin tissue using MBS7046078 at dilution 1:100
anti-EP300 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-EP300 antibody    Immunofluorescent analysis of MCF7 cells using MBS7046078 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-EP300 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for EP300. It may not necessarily be applicable to this product.
 NCBI GI #    50345997
 NCBI GeneID    2033
 NCBI Accession #    NP_001420.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_001429.3 [Other Products]
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 UniProt Primary Accession #    Q09472 [Other Products]
 UniProt Secondary Accession #    B1AKC2 [Other Products]
 UniProt Related Accession #    Q09472 [Other Products]
 Molecular Weight    264,161 Da
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 NCBI Official Full Name    histone acetyltransferase p300
 NCBI Official Synonym Full Names    E1A binding protein p300
 NCBI Official Symbol    EP300 [Similar Products]
 NCBI Official Synonym Symbols   
p300; KAT3B; RSTS2
[Similar Products]
 NCBI Protein Information    histone acetyltransferase p300
 UniProt Protein Name    Histone acetyltransferase p300
 UniProt Synonym Protein Names   
E1A-associated protein p300
 Protein Family    EP300-interacting inhibitor of differentiation
 UniProt Gene Name    EP300 [Similar Products]
 UniProt Synonym Gene Names    P300; p300 HAT [Similar Products]
 UniProt Entry Name    EP300_HUMAN
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 NCBI Summary for EP300    This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
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 UniProt Comments for EP300    p300: a histone acetyltransferase and transcriptional co-activator that regulates transcription via chromatin remodeling. Acetylates all four core histones in nucleosomes. Related to CPB (CREB-binding protein), and like CPB can stimulate transcription through activation of CREB. Specifically inhibited by the adenovirus oncoprotein E1A. A co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. Methylated at R580 and R604 in the KIX domain by CARM1, which blocks association with CREB, inhibits CREB signaling and activates the apoptotic response. Also methylated at R2142 by CARM1, which impairs interaction with NCoA2.

Protein type: Acetyltransferase; EC 2.3.1.48; Nuclear receptor co-regulator; Motility/polarity/chemotaxis; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 22q13.2

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: acetyltransferase activity; androgen receptor binding; beta-catenin binding; chromatin binding; damaged DNA binding; DNA binding; histone acetyltransferase activity; lysine N-acetyltransferase activity; nuclear hormone receptor binding; peptide N-acetyltransferase activity; protein binding; protein C-terminus binding; transcription activator binding; transcription coactivator activity; transcription factor binding; transferase activity, transferring acyl groups

Biological Process: apoptosis; circadian rhythm; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; fat cell differentiation; internal peptidyl-lysine acetylation; internal protein amino acid acetylation; N-terminal peptidyl-lysine acetylation; negative regulation of transcription from RNA polymerase II promoter; nervous system development; Notch signaling pathway; positive regulation of gene expression, epigenetic; positive regulation of interferon type I production; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription of target genes involved in unfolded protein response; protein amino acid acetylation; protein stabilization; regulation of autophagy; regulation of cell cycle; regulation of transcription, DNA-dependent; response to estrogen stimulus; response to hypoxia; stimulatory C-type lectin receptor signaling pathway; transcription-coupled nucleotide-excision repair

Disease: Colorectal Cancer; Rubinstein-taybi Syndrome 1; Rubinstein-taybi Syndrome 2
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 Research Articles on EP300    1. levels of p300 protein are temporally maintained in ligand-enhanced skeletal myocyte development. this maintenance of p300 protein is observed at the stage of myoblast differentiation, which coincides with an increase in Akt phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EP300 antibodyPathways associated with anti-EP300 antibody
 Reference Product  PubMed Publications
 TP53 antibody  >40 publications with EP300 and TP53
 HIF1A antibody  >39 publications with EP300 and HIF1A
 RELA antibody  >32 publications with EP300 and RELA
 SMAD3 antibody  >30 publications with EP300 and SMAD3
 MYB antibody  >20 publications with EP300 and MYB
 CREB1 antibody  >13 publications with EP300 and CREB1
 CARM1 antibody  >12 publications with EP300 and CARM1
 NCOA3 antibody  >8 publications with EP300 and NCOA3
 ESR1 antibody  >4 publications with EP300 and ESR1
 MYOD1 antibody  >2 publications with EP300 and MYOD1
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activation Of HOX Genes During Differentiation Pathway antibodies  Activation Of HOX Genes During Differentiation Pathway Diagram
 Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway antibodies  Activation Of Anterior HOX Genes In Hindbrain Development During Early Embryogenesis Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Attenuation Phase Pathway antibodies  Attenuation Phase Pathway Diagram
 B-WICH Complex Positively Regulates RRNA Expression Pathway antibodies  B-WICH Complex Positively Regulates RRNA Expression Pathway Diagram
 BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway antibodies  BMAL1:CLOCK,NPAS2 Activates Circadian Gene Expression Pathway Diagram
 C-MYB Transcription Factor Network Pathway antibodies  C-MYB Transcription Factor Network Pathway Diagram
Diseases associated with anti-EP300 antibodyOrgans/Tissues associated with anti-EP300 antibody
 Disease Name  Pubmed Publications
 Rubinstein-Taybi Syndrome Antibodies  >45 publications with EP300 and Rubinstein-Taybi Syndrome
 Breast Neoplasms Antibodies  >41 publications with EP300 and Breast Neoplasms
 Necrosis Antibodies  >39 publications with EP300 and Necrosis
 Inflammation Antibodies  >37 publications with EP300 and Inflammation
 Craniofacial Abnormalities Antibodies  >34 publications with EP300 and Craniofacial Abnormalities
 Cardiovascular Diseases Antibodies  >29 publications with EP300 and Cardiovascular Diseases
 Cell Transformation, Neoplastic Antibodies  >27 publications with EP300 and Cell Transformation, Neoplastic
 Lung Neoplasms Antibodies  >25 publications with EP300 and Lung Neoplasms
 Neoplasms, Experimental Antibodies  >24 publications with EP300 and Neoplasms, Experimental
 Colorectal Neoplasms Antibodies  >23 publications with EP300 and Colorectal Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >95 publications with EP300 and Blood
 Muscle Antibodies  >88 publications with EP300 and Muscle
 Heart Antibodies  >56 publications with EP300 and Heart
 Bone Antibodies  >50 publications with EP300 and Bone
 Liver Antibodies  >46 publications with EP300 and Liver
 Lung Antibodies  >43 publications with EP300 and Lung
 Brain Antibodies  >41 publications with EP300 and Brain
 Kidney Antibodies  >32 publications with EP300 and Kidney
 Nerve Antibodies  >28 publications with EP300 and Nerve
 Embryonic Tissue Antibodies  >26 publications with EP300 and Embryonic Tissue
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