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anti-EWSR1 antibody :: Rabbit EWSR1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9605901
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

EWSR1, Polyclonal Antibody

★Popular Item★
 Also Known As   

EWSR1 Antibody

 Product Synonym Names    bK984G1.4; bK984G1.4 Ewing sarcoma breakpoint region 1 protein; Ewing sarcoma breakpoint region 1; Ewing sarcoma breakpoint region 1 protein; Ewings sarcoma EWS Fli1 type 1 oncogene; EWS; EWS oncogene; EWS RNA binding protein 1; EWS_HUMAN; EWSR 1; Ewsr1; EWSR1 protein; RNA binding protein EWS; RNA-binding protein EWS
 Product Gene Name   

anti-EWSR1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q01844
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    EWSR1 antibody detects endogenous levels of total EWSR1
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide
 Subcellular Location    Nucleus. Cytoplasm. Cell Membrane. Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation.
 Tissue Specificity    Ubiquitous.
 Conjugation    Unconjugated
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-EWSR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-EWSR1 antibody

   Function: Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
Subunit Structure: Binds POLR2C, SF1, calmodulin and RNA. Interacts with PTK2B/FAK2 and TDRD3. Binds calmodulin in the presence, but not in the absence, of calcium ion.
Post-translational Modifications: Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8.
Similarity: EWS activation domain (EAD) functions as a potent activation domain in EFPS. EWSR1 binds POLR2C but not POLR2E or POLR2G, whereas the isolated EAD binds POLR2E and POLR2G but not POLR2C. Cis-linked RNA-binding domain (RBD) can strongly and specifically repress trans-activation by the EAD. Belongs to the RRM TET family.
 Applications Tested/Suitable for anti-EWSR1 antibody   

Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)

 Application Notes for anti-EWSR1 antibody    WB: 1:500-1:1000
IF/ICC: 1:100-1:500
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 Immunofluorescence (IF) of anti-EWSR1 antibody    MBS9605901 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
anti-EWSR1 antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-EWSR1 antibody    Western blot analysis of extracts from HeLa cells using EWSR1 antibody.The lane on the left is treated with the antigen-specific peptide.
anti-EWSR1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for EWSR1. It may not necessarily be applicable to this product.
 NCBI GI #    253970502
 NCBI GeneID    2130
 NCBI Accession #    NP_001156757.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001163285.1 [Other Products]
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 UniProt Primary Accession #    Q01844 [Other Products]
 UniProt Secondary Accession #    Q5THL0; Q92635; Q96FE8; Q96MN4; Q96MX4; Q9BWA2; B0QYK1 [Other Products]
 UniProt Related Accession #    Q01844 [Other Products]
 Molecular Weight    Observed: 68 kD
Predicted: 69 kDa
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 NCBI Official Full Name    RNA-binding protein EWS isoform 3
 NCBI Official Synonym Full Names    EWS RNA binding protein 1
 NCBI Official Symbol    EWSR1 [Similar Products]
 NCBI Official Synonym Symbols   
EWS; EWS-FLI1; bK984G1.4
[Similar Products]
 NCBI Protein Information    RNA-binding protein EWS
 UniProt Protein Name    RNA-binding protein EWS
 UniProt Synonym Protein Names   
EWS oncogene; Ewing sarcoma breakpoint region 1 protein
 Protein Family    RNA-binding protein
 UniProt Gene Name    EWSR1 [Similar Products]
 UniProt Synonym Gene Names    EWS [Similar Products]
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 NCBI Summary for EWSR1    This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009]
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 UniProt Comments for EWSR1    Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes.
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 Research Articles on EWSR1    1. ATG4B expression was observed markedly upregulated by EWS-FLI1 overexpression, and silencing of ATG4B dramatically inhibits autophagy in Ewing sarcoma cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EWSR1 antibodyPathways associated with anti-EWSR1 antibody
 Reference Product  PubMed Publications
 FLI1 antibody  >398 publications with EWSR1 and FLI1
 POU5F1 antibody  >14 publications with EWSR1 and POU5F1
 POU4F1 antibody  >4 publications with EWSR1 and POU4F1
 SF1 antibody  >4 publications with EWSR1 and SF1
 MSC antibody  >3 publications with EWSR1 and MSC
 CREBBP antibody  >2 publications with EWSR1 and CREBBP
 SNRPC antibody  >2 publications with EWSR1 and SNRPC
 POLR2G antibody  >1 publications with EWSR1 and POLR2G
 PCBP1 antibody  >1 publications with EWSR1 and PCBP1
 BTK antibody  >1 publications with EWSR1 and BTK
 Products by Pathway  Pathway Diagram
 BARD1 Signaling Events Pathway antibodies  BARD1 Signaling Events Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
 Transcriptional Misregulation In Cancer Pathway antibodies  Transcriptional Misregulation In Cancer Pathway Diagram
Diseases associated with anti-EWSR1 antibodyOrgans/Tissues associated with anti-EWSR1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >659 publications with EWSR1 and Neoplasms
 Sarcoma, Ewing Antibodies  >475 publications with EWSR1 and Sarcoma, Ewing
 Neuroectodermal Tumors, Primitive Antibodies  >157 publications with EWSR1 and Neuroectodermal Tumors, Primitive
 Cell Transformation, Neoplastic Antibodies  >77 publications with EWSR1 and Cell Transformation, Neoplastic
 Nervous System Diseases Antibodies  >38 publications with EWSR1 and Nervous System Diseases
 Neoplasm Metastasis Antibodies  >33 publications with EWSR1 and Neoplasm Metastasis
 Kidney Diseases Antibodies  >23 publications with EWSR1 and Kidney Diseases
 Kidney Neoplasms Antibodies  >22 publications with EWSR1 and Kidney Neoplasms
 Necrosis Antibodies  >20 publications with EWSR1 and Necrosis
 Brain Diseases Antibodies  >19 publications with EWSR1 and Brain Diseases
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >251 publications with EWSR1 and Bone
 Blood Antibodies  >40 publications with EWSR1 and Blood
 Nerve Antibodies  >33 publications with EWSR1 and Nerve
 Bone Marrow Antibodies  >31 publications with EWSR1 and Bone Marrow
 Brain Antibodies  >31 publications with EWSR1 and Brain
 Skin Antibodies  >29 publications with EWSR1 and Skin
 Muscle Antibodies  >29 publications with EWSR1 and Muscle
 Kidney Antibodies  >28 publications with EWSR1 and Kidney
 Thyroid Antibodies  >22 publications with EWSR1 and Thyroid
 Lung Antibodies  >22 publications with EWSR1 and Lung
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