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anti-NNE antibody :: Rabbit anti-Mouse Enolase, Non Neuronal (NNE) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2004811 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $110 +1 FREE 8GB USB
0.02 mg  /  $125 +1 FREE 8GB USB
0.05 mg  /  $175 +1 FREE 8GB USB
0.1 mg  /  $225 +1 FREE 8GB USB
0.2 mg  /  $330 +1 FREE 8GB USB
1 mg  /  $785 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Enolase, Non Neuronal (NNE), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to Enolase, Non Neuronal (NNE)

 Product Gene Name   

anti-NNE antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSG SEFELRRQAC -MSILRIHAR E IFDSRGNPTV EVDLYTAKGL FRAAVPSGAS TGIYEALELR DNDKTRFMGK GVSQAVEHIN KTIAPALVSK KVNVVEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN PEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGASSFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEALELLKTA IAKAGYTDQV VIGMDVAASE FYRSGKYDLD FKSPDDPSRY ITPDQLADLY KSFVQNYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AASEKSCNCL LLKVNQIGSV TESLQACKLA QSNGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQI LRIEEELGSK AKFAGRSFRN PLAK
 3D Structure    ModBase 3D Structure for P17182
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse
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 Specificity    The antibody is a rabbit polyclonal antibody raised against NNE. It has been selected for its ability to recognize NNE in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Recombinant NNE (Met1~Lys434) expressed in E.coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2055320
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 ISO Certification    Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.
 Supply Chain Verification    Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available. Please inquire.
 Other Notes    Small volumes of anti-NNE antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-NNE antibody    Knock-Out Validation Antibody
 Applications Tested/Suitable for anti-NNE antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

 Application Notes for anti-NNE antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) Knockout Verification of anti-NNE antibody    Knockout Verification: Lane 1: Wild-type MCF7 cell lysate
Lane 2: NNE knockout MCF7 cell lysate
Predicted MW: 36,47kDa ;Observed MW: 50kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse NNE Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-NNE antibody Western Blot (WB) Knockout Verification (WB) image
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 Western Blot (WB) of anti-NNE antibody    Western Blot; Sample: Mouse Placenta lysate; Primary Ab: 2ug/mL Rabbit Anti-Mouse NNE Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
anti-NNE antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-NNE antibody    Western Blot; Sample: Recombinant protein.
anti-NNE antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-NNE antibody    DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-NNE antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-NNE antibody    DAB staining on IHC-P; Samples: Mouse Liver Tissue)
anti-NNE antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for NNE. It may not necessarily be applicable to this product.
 NCBI GI #    70794816
 NCBI GeneID    433182
 NCBI Accession #    NP_001020559.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001025388.1 [Other Products]
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 UniProt Primary Accession #    P17182 [Other Products]
 UniProt Secondary Accession #    Q99KT7; Q9DCY7 [Other Products]
 Molecular Weight    47,141 Da
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 NCBI Official Full Name    enolase 1B, retrotransposed
 NCBI Official Synonym Full Names    enolase 1B, retrotransposed
 NCBI Official Symbol    Eno1b [Similar Products]
 NCBI Official Synonym Symbols   
Eno1; Gm5506; EG433182
[Similar Products]
 NCBI Protein Information    enolase 1B, retrotransposed
 UniProt Protein Name    Alpha-enolase
 UniProt Synonym Protein Names   
2-phospho-D-glycerate hydro-lyase; Enolase 1; Non-neural enolase; NNE
 UniProt Gene Name    Eno1 [Similar Products]
 UniProt Synonym Gene Names    Eno-1; NNE [Similar Products]
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 NCBI Summary for NNE    This gene may represent an evolving pseudogene of the alpha-enolase (enolase 1, alpha non-neuron) gene, which has multiple pseudogenes. This gene has an intact open reading frame as well as strong transcriptional support. The length of encoded protein is conserved, compared to the original enolase 1 protein. The exact function of this gene is unknown. [provided by RefSeq, Jul 2008]
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 UniProt Comments for NNE    ENO1: an abundant cytoplasmic enzyme with 2-phospho-D-glycerate hydro-lyase activity. A target of excess protein nitration in Alzheimer disease (AD) brain. Tau-crystallin, a structural lens protein, is produced from an alternative translation start in the enolase gene.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 4.2.1.11; Lyase; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 4 E2|4 81.24 cM

Cellular Component: cell surface; cytoplasm; cytosol; extracellular exosome; extracellular space; extrinsic component of plasma membrane; growth cone; membrane; membrane raft; myelin sheath; neuron projection; nucleus; phosphopyruvate hydratase complex; plasma membrane

Molecular Function: cadherin binding; enzyme binding; GTPase binding; heat shock protein binding; phosphopyruvate hydratase activity; protein heterodimerization activity; protein homodimerization activity; RNA binding

Biological Process: in utero embryonic development; positive regulation of binding
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-NNE antibody
 Products by Pathway  Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis / Gluconeogenesis Pathway antibodies  Glycolysis / Gluconeogenesis Pathway Diagram
 Glycolysis And Gluconeogenesis Pathway antibodies  Glycolysis And Gluconeogenesis Pathway Diagram
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