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anti-EGF antibody :: Rabbit Epidermal Growth Factor (EGF) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2006805 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Western Blot (WB)
Unit / Price
0.01 mg  /  $120 +1 FREE 8GB USB
0.02 mg  /  $140 +1 FREE 8GB USB
0.05 mg  /  $200 +1 FREE 8GB USB
0.1 mg  /  $260 +1 FREE 8GB USB
0.2 mg  /  $400 +1 FREE 8GB USB
1 mg  /  $950 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Epidermal Growth Factor (EGF), Polyclonal Antibody

★Popular Item★
 Also Known As   

Biotin-Linked Antibody to Epidermal Growth Factor (EGF)

 Product Gene Name   

anti-EGF antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- APFLIF SHGNSIFRID LEGTNHEQLV ADAGISVLMD FHYNEERIYW VDLERQLLQR VFLNGTRQEK VCNLEKNVSG MAINWINEEL IWSNQQEGTI TVTDMKGNNS RVLLSALKYP ANVAVDPVER LMFWSSVVAG SLHRADVTGV EVRLLLETSE EIAAVSLDVL DKRLFWIQYN REGGSSRICS CDYDGGSVHF SKHLTQHNVF AMSLFGDHIF YSTWKKKTIW VANKH
 Chromosome Location    Chromosome: 4; NC_000004.11 (110834040..110934118). Location: 4q25
 OMIM    131530
 3D Structure    ModBase 3D Structure for P01133
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Porcine (Pig)
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 Specificity    The antibody is a rabbit polyclonal antibody raised against EGF conjugated to biotin. It has been selected for its ability to recognize EGF in immunohistochemical staining andwestern blotting.
 Purity/Purification    Antigen-specific Affinity Chromatography.
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol
 Concentration    200ug/ml (lot specific)
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 Source    Antibody labeling
 Traits    Liquid
 Unconjugated Antibody    The unconjugated antibody version of this item is also available as catalog #MBS2002325
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 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of anti-EGF antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-EGF antibody    Biotin Antibody
 Applications Tested/Suitable for anti-EGF antibody   

WB; ICC; IHC-P; IHC-F; IF; ELISA.

 Application Notes for anti-EGF antibody    Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
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 Western Blot (WB) of anti-EGF antibody    (Figure. Western Blot; Sample: Recombinant protein.)
anti-EGF antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for EGF. It may not necessarily be applicable to this product.
 NCBI GI #    46242544
 NCBI GeneID    1950
 NCBI Accession #    AAS83395.1 [Other Products]
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 UniProt Primary Accession #    P01133 [Other Products]
 UniProt Secondary Accession #    Q52LZ6; B4DRK7; E7EVD2; E9PBF0 [Other Products]
 UniProt Related Accession #    P01133 [Other Products]
 Molecular Weight    133,994 Da
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 NCBI Official Full Name    epidermal growth factor
 NCBI Official Synonym Full Names    epidermal growth factor
 NCBI Official Symbol    EGF [Similar Products]
 NCBI Official Synonym Symbols   
URG; HOMG4
[Similar Products]
 NCBI Protein Information    pro-epidermal growth factor; beta-urogastrone
 UniProt Protein Name    Pro-epidermal growth factor
 UniProt Synonym Protein Names   
Urogastrone
 Protein Family    Pro-epidermal growth factor
 UniProt Gene Name    EGF [Similar Products]
 UniProt Synonym Gene Names    EGF [Similar Products]
 UniProt Entry Name    EGF_HUMAN
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 NCBI Summary for EGF    This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2010]
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 UniProt Comments for EGF    EGF: EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6. Defects in EGF are the cause of hypomagnesemia type 4 (HOMG4); also known as renal hypomagnesemia normocalciuric. HOMG4 is a disorder characterized by massive renal hypomagnesemia and normal levels of serum calcium and calcium excretion. Clinical features include seizures, mild-to mederate psychomotor retardation, and brisk tendon reflexes. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hormone; Membrane protein, integral; Ligand, receptor tyrosine kinase

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space; lysosomal membrane; integral to membrane; plasma membrane; extracellular region

Molecular Function: transmembrane receptor protein tyrosine kinase activator activity; protein binding; growth factor activity; calcium ion binding; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of epidermal growth factor receptor signaling pathway; platelet activation; phosphoinositide-mediated signaling; transmembrane receptor protein tyrosine kinase activation (dimerization); peptidyl-tyrosine phosphorylation; fibroblast growth factor receptor signaling pathway; positive regulation of mitosis; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of transcription, DNA-dependent; signal transduction; positive regulation of MAP kinase activity; platelet degranulation; branching morphogenesis of a tube; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; innate immune response; negative regulation of secretion; angiogenesis; blood coagulation; DNA replication; positive regulation of phosphorylation; positive regulation of DNA binding; STAT protein nuclear translocation; positive regulation of granule cell precursor proliferation

Disease: Hypomagnesemia 4, Renal
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 Research Articles on EGF    1. Six apolipoproteins (APOA1, APOA2, APOB, APOC2, APOC3, and APOE) were able to differentiate bladder cancer from hernia. SAA4 was significantly increased in bladder cancer subgroups, whereas ProEGF was significantly decreased in bladder cancer subgroups.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-EGF antibodyPathways associated with anti-EGF antibody
 Reference Product  PubMed Publications
 EGFR antibody  >5181 publications with EGF and EGFR
 ERBB2 antibody  >1292 publications with EGF and ERBB2
 SRC antibody  >1187 publications with EGF and SRC
 GRB2 antibody  >354 publications with EGF and GRB2
 STAT3 antibody  >258 publications with EGF and STAT3
 ERBB3 antibody  >251 publications with EGF and ERBB3
 ERBB4 antibody  >207 publications with EGF and ERBB4
 ADAM17 antibody  >93 publications with EGF and ADAM17
 FN1 antibody  >7 publications with EGF and FN1
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Arf6 Signaling Events Pathway antibodies  Arf6 Signaling Events Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Bladder Cancer Pathway antibodies  Bladder Cancer Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 Constitutive PI3K/AKT Signaling In Cancer Pathway antibodies  Constitutive PI3K/AKT Signaling In Cancer Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 Cytokine-cytokine Receptor Interaction Pathway antibodies  Cytokine-cytokine Receptor Interaction Pathway Diagram
 DAP12 Interactions Pathway antibodies  DAP12 Interactions Pathway Diagram
 DAP12 Signaling Pathway antibodies  DAP12 Signaling Pathway Diagram
Diseases associated with anti-EGF antibodyOrgans/Tissues associated with anti-EGF antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >7846 publications with EGF and Neoplasms
 Breast Neoplasms Antibodies  >1564 publications with EGF and Breast Neoplasms
 Adenocarcinoma Antibodies  >1236 publications with EGF and Adenocarcinoma
 Carcinoma, Squamous Cell Antibodies  >1141 publications with EGF and Carcinoma, Squamous Cell
 Necrosis Antibodies  >947 publications with EGF and Necrosis
 Cell Transformation, Neoplastic Antibodies  >847 publications with EGF and Cell Transformation, Neoplastic
 Neoplasms, Experimental Antibodies  >818 publications with EGF and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >756 publications with EGF and Lung Neoplasms
 Inflammation Antibodies  >645 publications with EGF and Inflammation
 Neoplasm Metastasis Antibodies  >576 publications with EGF and Neoplasm Metastasis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >1801 publications with EGF and Brain
 Lung Antibodies  >1664 publications with EGF and Lung
 Kidney Antibodies  >1564 publications with EGF and Kidney
 Skin Antibodies  >1339 publications with EGF and Skin
 Muscle Antibodies  >1284 publications with EGF and Muscle
 Embryonic Tissue Antibodies  >1090 publications with EGF and Embryonic Tissue
 Eye Antibodies  >903 publications with EGF and Eye
 Intestine Antibodies  >880 publications with EGF and Intestine
 Mouth Antibodies  >846 publications with EGF and Mouth
 Placenta Antibodies  >456 publications with EGF and Placenta
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