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anti-ERBB2 antibody :: Rabbit anti-Human, Rat ErbB2, Tyr1248 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS613410 anti-ERBB2 antibody
Unit / Price
0.1 mL  /  $610 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ErbB2, Tyr1248, Polyclonal Antibody

 Also Known As   

ErbB2, phosphorylated, Tyr1248 (c-erbB2, HER2, neu Oncoprotein)

 Product Synonym Names    Anti -ErbB2, phosphorylated, Tyr1248 (c-erbB2, HER2, neu Oncoprotein)
 Product Gene Name   

anti-ERBB2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 17; NC_000017.10 (37844393..37884915). Location: 17q11.2-q12; 17q21.1
 3D Structure    ModBase 3D Structure for Q9NP09
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Rat
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 Specificity    Recognizes the activated form of human Erb B2/HER-2 oncoprotein at 185 kD, phosphorylated at Tyr1248. Species Crossreactivity: human. Predicted to crossreact with rat based on conservation of immunogenic sequence.
 Purity/Purification    Affinity Purified
Purified by immunoaffinity chromatography.
 Form/Format    Supplied as a liquid in 0.2M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide.
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 Immunogen    Synthetic phosphopeptide, residues 1243-1255, corresponding to a major auto-phosphorylation (Tyr1248) site of human Erb B2/HER-2 (C-AENPEpYLGLDVPV).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
 Other Notes    Small volumes of anti-ERBB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-ERBB2 antibody    Antibodies; Abs to Oncoproteins
 Applications Tested/Suitable for anti-ERBB2 antibody   

Western Blot (WB)

 Application Notes for anti-ERBB2 antibody    Suitable for use in Western Blot.
Dilution: Western Blot: 1:2000 -1:8000 dilutions detects activated Erb B2/HER-2 in RIPA lysates from heregulin stimulated MCF-7 breast carcinoma cells. Lysates from non-stimulated and heregulin-stimulated MCF-7 cells were resolved by electrophoresis, transferred to nitrocellulose and probed with E3451-25 (1:2000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilution determined by the researcher.
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NCBI/Uniprot data below describe general gene information for ERBB2. It may not necessarily be applicable to this product.
 NCBI GI #    6857751
 NCBI GeneID    2064
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 UniProt Primary Accession #    Q9NP09 [Other Products]
 UniProt Related Accession #    P04626; Q4H1F1; Q4H1F2; Q9NP09; Q9UK79 [Other Products]
 Molecular Weight    4,204 Da [Similar Products]
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 NCBI Official Full Name    ERBB2
 NCBI Official Synonym Full Names    v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
 NCBI Official Symbol    ERBB2 [Similar Products]
 NCBI Official Synonym Symbols   
NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
[Similar Products]
 NCBI Protein Information    receptor tyrosine-protein kinase erbB-2; herstatin; p185erbB2; proto-oncogene Neu; c-erb B2/neu protein; proto-oncogene c-ErbB-2; metastatic lymph node gene 19 protein; tyrosine kinase-type cell surface receptor HER2; neuroblastoma/glioblastoma derived oncogene homolog; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog)
 UniProt Protein Name    ERBB2
 Protein Family    Receptor tyrosine-protein kinase
 UniProt Gene Name    ERBB2 [Similar Products]
 UniProt Entry Name    Q9NP09_HUMAN
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 NCBI Summary for ERBB2    This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq]
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 UniProt Comments for ERBB2    HER2: a proto-oncogenic receptor tyrosine kinase of the EGFR family. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. Not activated by EGF, TGF- alpha and amphiregulin. Amplified in breast cancer. Overexpression induces constitutive activity, and the gene is amplified or overexpressed in up to 30% of breast cancers, correlating with poor survival. The antibody Herceptin is approved for treatment of metastatic breast cancer with HER2 amplification/overexpression. Somatic mutations seen in 4% of lung cancers and also in breast, gastric, ovarian cancer and glioblastoma. One SNP shows predisposition to breast and gastric cancer. Inhibitors: Herceptin, lapatinib, PKI-166, EKB-569, CI-1033.

Protein type: Protein kinase, tyrosine (receptor); Membrane protein, integral; Protein kinase, TK; Oncoprotein; Kinase, protein; EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: perinuclear region of cytoplasm; basolateral plasma membrane; apical plasma membrane; cytoplasm; plasma membrane; integral to membrane; endosome membrane; cytoplasmic vesicle; nucleus; receptor complex

Molecular Function: protein C-terminus binding; protein dimerization activity; identical protein binding; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; protein phosphatase binding; protein binding; transmembrane receptor activity; ErbB-3 class receptor binding; protein heterodimerization activity; protein-tyrosine kinase activity; growth factor binding; ATP binding

Biological Process: myelination; axon guidance; phosphoinositide 3-kinase cascade; positive regulation of cell adhesion; peptidyl-tyrosine phosphorylation; positive regulation of translation; wound healing; nerve growth factor receptor signaling pathway; heart development; protein amino acid autophosphorylation; motor axon guidance; signal transduction; enzyme linked receptor protein signaling pathway; protein amino acid phosphorylation; positive regulation of transcription from RNA polymerase I promoter; positive regulation of MAP kinase activity; cell surface receptor linked signal transduction; oligodendrocyte differentiation; neuromuscular junction development; negative regulation of immature T cell proliferation in the thymus; epidermal growth factor receptor signaling pathway; regulation of microtubule-based process; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; transcription, DNA-dependent; regulation of angiogenesis; positive regulation of cell growth; peripheral nervous system development; cell proliferation; positive regulation of transcription from RNA polymerase III promoter; innate immune response; positive regulation of protein amino acid phosphorylation; positive regulation of epithelial cell proliferation; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Gastric Cancer; Lung Cancer; Glioma Susceptibility 1
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 Research Articles on ERBB2    1. the HER pathway is blocked by lapatinib and trastuzumab in HER2/neu-overexpressing breast tumor xenografts
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ERBB2 antibodyPathways associated with anti-ERBB2 antibody
 Reference Product  PubMed Publications
 EGFR antibody  >2276 publications with ERBB2 and EGFR
 EGF antibody  >1194 publications with ERBB2 and EGF
 ERBB3 antibody  >644 publications with ERBB2 and ERBB3
 ERBB4 antibody  >425 publications with ERBB2 and ERBB4
 SRC antibody  >327 publications with ERBB2 and SRC
 CTNNB1 antibody  >140 publications with ERBB2 and CTNNB1
 HLA-A antibody  >126 publications with ERBB2 and HLA-A
 GRB2 antibody  >103 publications with ERBB2 and GRB2
 MUC1 antibody  >97 publications with ERBB2 and MUC1
 GRB7 antibody  >64 publications with ERBB2 and GRB7
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 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
 Endometrial Cancer Pathway antibodies  Endometrial Cancer Pathway Diagram
Diseases associated with anti-ERBB2 antibodyOrgans/Tissues associated with anti-ERBB2 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13956 publications with ERBB2 and Neoplasms
 Breast Neoplasms Antibodies  >9506 publications with ERBB2 and Breast Neoplasms
 Skin Diseases Antibodies  >8799 publications with ERBB2 and Skin Diseases
 Carcinoma Antibodies  >5481 publications with ERBB2 and Carcinoma
 Adenocarcinoma Antibodies  >2771 publications with ERBB2 and Adenocarcinoma
 Neoplasm Metastasis Antibodies  >2187 publications with ERBB2 and Neoplasm Metastasis
 Neoplasms, Experimental Antibodies  >1434 publications with ERBB2 and Neoplasms, Experimental
 Carcinoma, Ductal, Breast Antibodies  >1420 publications with ERBB2 and Carcinoma, Ductal, Breast
 Lymphatic Metastasis Antibodies  >1008 publications with ERBB2 and Lymphatic Metastasis
 Lung Neoplasms Antibodies  >969 publications with ERBB2 and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >1613 publications with ERBB2 and Blood
 Lymph Node Antibodies  >1352 publications with ERBB2 and Lymph Node
 Lung Antibodies  >1027 publications with ERBB2 and Lung
 Stomach Antibodies  >430 publications with ERBB2 and Stomach
 Prostate Antibodies  >418 publications with ERBB2 and Prostate
 Brain Antibodies  >415 publications with ERBB2 and Brain
 Liver Antibodies  >387 publications with ERBB2 and Liver
 Mammary Gland Antibodies  >330 publications with ERBB2 and Mammary Gland
 Bone Antibodies  >323 publications with ERBB2 and Bone
 Heart Antibodies  >284 publications with ERBB2 and Heart
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