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anti-FAK antibody :: Rabbit FAK Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9503025
Western Blot (WB)
Unit / Price
0.05 mg  /  $225 +1 FREE 8GB USB
0.1 mg  /  $310 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FAK, Polyclonal Antibody

★Popular Item★
 Also Known As   

FAK (Phospho-Tyr397) Antibody

 Product Synonym Names    FADK 1; FAK1; Focal adhesion kinase 1; pp125FAK; Protein- tyrosine kinase 2; PTK2
 Product Gene Name   

anti-FAK antibody

[Similar Products]
 Product Synonym Gene Name    PTK2 [Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    L13616 mRNA
 3D Structure    ModBase 3D Structure for Q05397
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    FAK (Phospho-Tyr397) Antibody detects endogenous levels of FAK only when phosphorylated at Tyr397.
 Purity/Purification    Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
 Form/Format    Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1 mg/ml (lot specific)
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 Target Modification    Phospho
 Modification Sites    H:Y397; M:Y397; R:Y397
 Immunogen    The antiserum was produced against synthesized peptide derived from human FAK around the phosphorylation site of Tyr397.
 Immunogen Range    364-413
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 Preparation and Storage    Stable at -20 degree C for at least 1 year.
 Other Notes    Small volumes of anti-FAK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-FAK antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-FAK antibody    WB: 1:500~1:1000
ELISA: 1:5000
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 Western Blot (WB) of anti-FAK antibody    Western blot analysis of lysates from 293 cells treated with EGF 200ng/ml 30', using FAK (Phospho-Tyr397) Antibody. The lane on the right is blocked with the phospho peptide.
anti-FAK antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for FAK. It may not necessarily be applicable to this product.
 NCBI GI #    313851044
 NCBI GeneID    5747
 NCBI Accession #    NP_001186578.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001199649.1 [Other Products]
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 UniProt Primary Accession #    Q05397 [Other Products]
 UniProt Secondary Accession #    Q14291; Q8IYN9; Q9UD85; B4E2N6; F5H4S4; J3QT16 [Other Products]
 UniProt Related Accession #    Q05397 [Other Products]
 Molecular Weight    119 kDa
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 NCBI Official Full Name    focal adhesion kinase 1 isoform c
 NCBI Official Synonym Full Names    protein tyrosine kinase 2
 NCBI Official Symbol    PTK2 [Similar Products]
 NCBI Official Synonym Symbols   
FAK; FADK; FAK1; FRNK; PPP1R71; p125FAK; pp125FAK
[Similar Products]
 NCBI Protein Information    focal adhesion kinase 1
 UniProt Protein Name    Focal adhesion kinase 1
 UniProt Synonym Protein Names   
Focal adhesion kinase-related nonkinase; FRNK; Protein phosphatase 1 regulatory subunit 71; PPP1R71; Protein-tyrosine kinase 2; p125FAK; pp125FAK
 UniProt Gene Name    PTK2 [Similar Products]
 UniProt Synonym Gene Names    FAK; FAK1; FADK 1; FRNK; PPP1R71 [Similar Products]
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 NCBI Summary for FAK    This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2017]
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 UniProt Comments for FAK    Non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. Required for early embryonic development and placenta development. Required for embryonic angiogenesis, normal cardiomyocyte migration and proliferation, and normal heart development. Regulates axon growth and neuronal cell migration, axon branching and synapse formation; required for normal development of the nervous system. Plays a role in osteogenesis and differentiation of osteoblasts. Functions in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade. Promotes activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. Promotes localized and transient activation of guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs), and thereby modulates the activity of Rho family GTPases. Signaling via CAS family members mediates activation of RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ACTN1, ARHGEF7, GRB7, RET and WASL. Promotes phosphorylation of PXN and STAT1; most likely PXN and STAT1 are phosphorylated by a SRC family kinase that is recruited to autophosphorylated PTK2/FAK1, rather than by PTK2/FAK1 itself. Promotes phosphorylation of BCAR1; GIT2 and SHC1; this requires both SRC and PTK2/FAK1. Promotes phosphorylation of BMX and PIK3R1. Isoform 6 (FRNK) does not contain a kinase domain and inhibits PTK2/FAK1 phosphorylation and signaling. Its enhanced expression can attenuate the nuclear accumulation of LPXN and limit its ability to enhance serum response factor (SRF)-dependent gene transcription.
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 Research Articles on FAK    1. BKCa has a role in promoting growth and metastasis of prostate cancer through facilitating the coupling between alphavbeta3 integrin and FAK
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-FAK antibodyPathways associated with anti-FAK antibody
 Reference Product  PubMed Publications
 SRC antibody  >574 publications with FAK and SRC
 CRK antibody  >151 publications with FAK and CRK
 BCAR1 antibody  >102 publications with FAK and BCAR1
 FYN antibody  >69 publications with FAK and FYN
 PTK2B antibody  >64 publications with FAK and PTK2B
 GRB2 antibody  >59 publications with FAK and GRB2
 EGFR antibody  >48 publications with FAK and EGFR
 CSK antibody  >48 publications with FAK and CSK
 SYK antibody  >25 publications with FAK and SYK
 PTPN11 antibody  >25 publications with FAK and PTPN11
 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Angiogenesis Pathway antibodies  Angiogenesis Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
 Axon Guidance Pathway antibodies  Axon Guidance Pathway Diagram
Diseases associated with anti-FAK antibodyOrgans/Tissues associated with anti-FAK antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >757 publications with FAK and Neoplasms
 Neoplasm Invasiveness Antibodies  >176 publications with FAK and Neoplasm Invasiveness
 Neoplasm Metastasis Antibodies  >152 publications with FAK and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >116 publications with FAK and Breast Neoplasms
 Lung Diseases Antibodies  >77 publications with FAK and Lung Diseases
 Neoplasms, Experimental Antibodies  >76 publications with FAK and Neoplasms, Experimental
 Lung Neoplasms Antibodies  >74 publications with FAK and Lung Neoplasms
 Liver Diseases Antibodies  >72 publications with FAK and Liver Diseases
 Liver Neoplasms Antibodies  >70 publications with FAK and Liver Neoplasms
 Cardiovascular Diseases Antibodies  >69 publications with FAK and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Vascular Antibodies  >360 publications with FAK and Vascular
 Blood Antibodies  >309 publications with FAK and Blood
 Muscle Antibodies  >225 publications with FAK and Muscle
 Brain Antibodies  >161 publications with FAK and Brain
 Kidney Antibodies  >150 publications with FAK and Kidney
 Lung Antibodies  >126 publications with FAK and Lung
 Bone Antibodies  >118 publications with FAK and Bone
 Liver Antibodies  >113 publications with FAK and Liver
 Embryonic Tissue Antibodies  >94 publications with FAK and Embryonic Tissue
 Heart Antibodies  >84 publications with FAK and Heart
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