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anti-FOXO1 antibody :: Rabbit FOXO1/3/4-pan Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9601969
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

FOXO1/3/4-pan, Polyclonal Antibody

★Popular Item★
 Also Known As   

FOXO1/3/4-pan Antibody

 Product Synonym Names    FKH 1; FKH1; FKHR; Forkhead (Drosophila) homolog 1 (rhabdomyosarcoma); Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FoxO transcription factor; foxo1; FOXO1_HUMAN; FOXO1A; OTTHUMP00000018301; AFX; AFX1; Afxh; ALL1-fused gene from X chromosome; Fork head domain transcription factor AFX1; Forkhead box O4; Forkhead box protein O4; FOXO 4; Foxo4; FOXO4_HUMAN; MGC117660; MGC120490; Mixed lineage leukemia, translocated to, 7; MLLT7; Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila); translocated to, 7; Myeloid/lymphoid or mixed lineage leukemia, translocated to, 7; RGD1561201
 Product Gene Name   

anti-FOXO1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q12778
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Specificity    FOXO1/3/4-pan antibody detects endogenous levels of total FOXO1/3/4-pan
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human FOXO1/3/4-pan
 Subcellular Location    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
 Tissue Specificity    Ubiquitous.
 Conjugation    Unconjugated
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-FOXO1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-FOXO1 antibody

   Description: FOXO4 transcription factor AFX1 containing 1 fork-head domain. May play a role in the insulin signaling pathway. Involved in acute leukemias by a chromosomal translocation t(X; 11)(q13; q23) that involves MLLT7 and MLL/HRX. The result is a rogue activator protein.
Function: Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling (By similarity).
Subunit Structure: Interacts with LRPPRC. Interacts with RUNX2; the interaction inhibits RUNX2 transcriptional activity and mediates the IGF1/insulin-dependent BGLAP expression in osteoblasts Interacts with PPP2R1A; the interaction regulates the dephosphorylation of FOXO1 at Thr-24 and Ser-256 leading to its nuclear import. Interacts (acetylated form) with PPARG. Interacts with XBP1 isoform 2; this interaction is direct and leads to FOXO1 ubiquitination and degradation via the proteasome pathway (By similarity). Interacts with NLK. Interacts with SIRT1; the interaction results in the deacetylation of FOXO1 leading to activation of FOXO1-mediated transcription of genes involved in DNA repair and stress resistance. Binds to CDK1. Interacts with the 14-3-3 proteins, YWHAG and YWHAZ; the interactions require insulin-stimulated phosphorylation on Thr-24, promote nuclear exit and loss of transcriptional activity. Interacts with SKP2; the interaction ubiquitinates FOXO1 leading to its proteosomal degradation. The interaction requires the presence of KRIT1. Interacts (via the C-terminal half) with ATF4 (via its DNA-binding domain); the interaction occurs in osteoblasts, regulates glucose homeostasis via suppression of beta-cell proliferation and subsequent decrease in insulin production. Interacts with PRMT1; the interaction methylates FOXO1, prevents PKB/AKT1 phosphorylation and retains FOXO1 in the nucleus. Interacts with EP300 and CREBBP; the interactions acetylate FOXO1. Interacts with SIRT2; the interaction is disrupted in response to oxidative stress or serum deprivation, leading to increased level of acetylated FOXO1, which promotes stress-induced autophagy by stimulating E1-like activating enzyme ATG7. Interacts (acetylated form) with ATG7; the interaction is increased in response to oxidative stress or serum deprivation and promotes the autophagic process leading to cell death. Interacts (via the Fork-head domain) with CEBPA; the interaction increases when FOXO1 is deacetylated. Interacts with WDFY2. Forms a complex with WDFY2 and AKT1 (By similarity).
Post-translational Modifications: Phosphorylation by NLK promotes nuclear export and inhibits the transcriptional activity. In response to growth factors, phosphorylation on Thr-24, Ser-256 and Ser-322 by PKB/AKT1 promotes nuclear export and inactivation of transactivational activity. Phosphorylation on Thr-24 is required for binding 14-3-3 proteins. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24 and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CDK1, leading to nuclear exclusion and loss of function. Stress signals, such as response to oxygen or nitric oxide, attenuate the PKB/AKT1-mediated phosphorylation leading to nuclear retention. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Dephosphorylated on Thr-24 and Ser-256 by PP2A in beta-cells under oxidative stress leading to nuclear retention (By similarity). Phosphorylation of Ser-249 by CDK1 disrupts binding of 14-3-3 proteins leading to nuclear accumulation and has no effect on DNA-binding nor transcriptional activity. Phosphorylation by STK4/MST1 on Ser-212, upon oxidative stress, inhibits binding to 14-3-3 proteins and nuclear export. Acetylated. Acetylation at Lys-262, Lys-265 and Lys-274 are necessary for autophagic cell death induction. Deacetylated by SIRT2 in response to oxidative stress or serum deprivation, thereby negatively regulating FOXO1-mediated autophagic cell death. Ubiquitinated by SRT2. Ubiquitination leads to proteasomal degradation. Methylation inhibits AKT1-mediated phosphorylation at Ser-256 and is increased by oxidative stress. Once in the nucleus, acetylated by CREBBP/EP300. Acetylation diminishes the interaction with target DNA and attenuates the transcriptional activity. It increases the phosphorylation at Ser-256. Deacetylation by SIRT1 results in reactivation of the transcriptional activity. Oxidative stress by hydrogen peroxide treatment appears to promote deacetylation and uncoupling of insulin-induced phosphorylation. By contrast, resveratrol acts independently of acetylation.
 Applications Tested/Suitable for anti-FOXO1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-FOXO1 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
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 Immunohistochemistry (IHC) of anti-FOXO1 antibody    MBS9601969 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-FOXO1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-FOXO1 antibody    Western blot analysis of FOXO1/3/4-pan expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.
anti-FOXO1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for FOXO1. It may not necessarily be applicable to this product.
 NCBI GI #    9257222
 NCBI GeneID    2308
 NCBI Accession #    NP_002006.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_002015.3 [Other Products]
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 UniProt Primary Accession #    Q12778 [Other Products]
 UniProt Secondary Accession #    O43523; Q5VYC7; Q6NSK6 [Other Products]
 UniProt Related Accession #    Q12778 [Other Products]
 Molecular Weight    Observed: 78 kDa
Predicted: 70 kDa
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 NCBI Official Full Name    forkhead box protein O1
 NCBI Official Synonym Full Names    forkhead box O1
 NCBI Official Symbol    FOXO1 [Similar Products]
 NCBI Official Synonym Symbols   
FKH1; FKHR; FOXO1A
[Similar Products]
 NCBI Protein Information    forkhead box protein O1
 UniProt Protein Name    Forkhead box protein O1
 UniProt Synonym Protein Names   
Forkhead box protein O1A; Forkhead in rhabdomyosarcoma
 Protein Family    Forkhead box protein
 UniProt Gene Name    FOXO1 [Similar Products]
 UniProt Synonym Gene Names    FKHR; FOXO1A [Similar Products]
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 NCBI Summary for FOXO1    This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008]
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 UniProt Comments for FOXO1    Transcription factor that is the main target of insulin signaling and regulates metabolic homeostasis in response to oxidative stress. Binds to the insulin response element (IRE) with consensus sequence 5'-TT[G/A]TTTTG-3' and the related Daf-16 family binding element (DBE) with consensus sequence 5'-TT[G/A]TTTAC-3'. Activity suppressed by insulin. Main regulator of redox balance and osteoblast numbers and controls bone mass. Orchestrates the endocrine function of the skeleton in regulating glucose metabolism. Acts synergistically with ATF4 to suppress osteocalcin/BGLAP activity, increasing glucose levels and triggering glucose intolerance and insulin insensitivity. Also suppresses the transcriptional activity of RUNX2, an upstream activator of osteocalcin/BGLAP. In hepatocytes, promotes gluconeogenesis by acting together with PPARGC1A and CEBPA to activate the expression of genes such as IGFBP1, G6PC and PCK1. Important regulator of cell death acting downstream of CDK1, PKB/AKT1 and STK4/MST1. Promotes neural cell death. Mediates insulin action on adipose tissue. Regulates the expression of adipogenic genes such as PPARG during preadipocyte differentiation and, adipocyte size and adipose tissue-specific gene expression in response to excessive calorie intake. Regulates the transcriptional activity of GADD45A and repair of nitric oxide-damaged DNA in beta-cells. Required for the autophagic cell death induction in response to starvation or oxidative stress in a transcription-independent manner. Mediates the function of MLIP in cardiomyocytes hypertrophy and cardiac remodeling ().
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 Research Articles on FOXO1    1. In particular, we discuss molecular mechanisms that might determine the switch between pro-apoptotic and pro-survival effects of FOXO1 and their interplay with specific differentiation programs.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-FOXO1 antibodyPathways associated with anti-FOXO1 antibody
 Reference Product  PubMed Publications
 AKT1 antibody  >119 publications with FOXO1 and AKT1
 SIRT1 antibody  >111 publications with FOXO1 and SIRT1
 PPARG antibody  >38 publications with FOXO1 and PPARG
 CTNNB1 antibody  >27 publications with FOXO1 and CTNNB1
 CDKN1A antibody  >21 publications with FOXO1 and CDKN1A
 SRC antibody  >15 publications with FOXO1 and SRC
 SMAD3 antibody  >12 publications with FOXO1 and SMAD3
 DYRK1A antibody  >7 publications with FOXO1 and DYRK1A
 FOXG1 antibody  >7 publications with FOXO1 and FOXG1
 HNF4A antibody  >7 publications with FOXO1 and HNF4A
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 AKT Phosphorylates Targets In The Nucleus Pathway antibodies  AKT Phosphorylates Targets In The Nucleus Pathway Diagram
 AKT-mediated Inactivation Of FOXO1A Pathway antibodies  AKT-mediated Inactivation Of FOXO1A Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Androgen Receptor Signaling Pathway antibodies  Androgen Receptor Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 B Cell Receptor Signaling Pathway antibodies  B Cell Receptor Signaling Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Class I PI3K Signaling Events Mediated By Akt Pathway antibodies  Class I PI3K Signaling Events Mediated By Akt Pathway Diagram
Diseases associated with anti-FOXO1 antibodyOrgans/Tissues associated with anti-FOXO1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >404 publications with FOXO1 and Neoplasms
 Insulin Resistance Antibodies  >227 publications with FOXO1 and Insulin Resistance
 Rhabdomyosarcoma, Alveolar Antibodies  >157 publications with FOXO1 and Rhabdomyosarcoma, Alveolar
 Atrophy Antibodies  >93 publications with FOXO1 and Atrophy
 Hyperglycemia Antibodies  >81 publications with FOXO1 and Hyperglycemia
 Fatty Liver Antibodies  >77 publications with FOXO1 and Fatty Liver
 Inflammation Antibodies  >75 publications with FOXO1 and Inflammation
 Cardiovascular Diseases Antibodies  >69 publications with FOXO1 and Cardiovascular Diseases
 Liver Diseases Antibodies  >68 publications with FOXO1 and Liver Diseases
 Necrosis Antibodies  >65 publications with FOXO1 and Necrosis
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >366 publications with FOXO1 and Muscle
 Blood Antibodies  >299 publications with FOXO1 and Blood
 Liver Antibodies  >288 publications with FOXO1 and Liver
 Nerve Antibodies  >221 publications with FOXO1 and Nerve
 Brain Antibodies  >121 publications with FOXO1 and Brain
 Vascular Antibodies  >119 publications with FOXO1 and Vascular
 Pancreas Antibodies  >113 publications with FOXO1 and Pancreas
 Heart Antibodies  >74 publications with FOXO1 and Heart
 Bone Antibodies  >65 publications with FOXO1 and Bone
 Embryonic Tissue Antibodies  >52 publications with FOXO1 and Embryonic Tissue
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