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anti-GAD1 antibody :: Goat GAD1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS421748
Western Blot (WB)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GAD1, Polyclonal Antibody

 Also Known As   

Goat anti-GAD1 (isoform GAD67) Antibody

 Product Synonym Names    Goat Anti-GAD25 / GAD67 Antibody; GAD1; glutamate decarboxylase 1 (brain, 67kDa); FLJ45882; GAD; SCP; OTTHUMP00000041055; glutamate decarboxylase 1; glutamate decarboxylase 1 (brain, 67kD); GAD67; GAD25; GAD1 antibody; glutamate decarboxylase 1 (brain; 67kDa) antibody; FLJ45882 antibody; GAD antibody; SCP antibody; OTTHUMP00000041055 antibody; glutamate decarboxylase 1 antibody; glutamate decarboxylase 1 (brain; 67kD) antibody; GAD67 antibody; GAD25 antibody; GAD1 (isoform GAD67)
 Product Gene Name   

anti-GAD1 antibody

[Similar Products]
 Antibody/Peptide Pairs    GAD1 peptide (MBS428399) is used for blocking the activity of GAD1 antibody (MBS421748)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    PDSPQRREKL HK
 OMIM    603513
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.
 Epitope    Internal region
 Note    This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-GAD1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-GAD1 antibody   

Peptide ELISA (EIA), Western Blot (WB)

 Application Notes for anti-GAD1 antibody    Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Approx 65kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 66.9kDa according to NP_000808.2). Recommended concentration: 0.5-1.5ug/ml.
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 Western Blot (WB) of anti-GAD1 antibody    (0.5ug/ml) staining of Human Brain (Cerebral Cortex) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
anti-GAD1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for GAD1. It may not necessarily be applicable to this product.
 NCBI GI #    58331246
 NCBI GeneID    2571
 NCBI Accession #    NP_000808.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000817.2 [Other Products]
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 UniProt Secondary Accession #    Q49AK1; Q53TQ7; Q9BU91; Q9UHH4 [Other Products]
 UniProt Related Accession #    Q99259 [Other Products]
 Molecular Weight    47,440 Da
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 NCBI Official Full Name    glutamate decarboxylase 1 isoform GAD67
 NCBI Official Synonym Full Names    glutamate decarboxylase 1
 NCBI Official Symbol    GAD1 [Similar Products]
 NCBI Official Synonym Symbols   
GAD; SCP; CPSQ1
[Similar Products]
 NCBI Protein Information    glutamate decarboxylase 1
 UniProt Protein Name    Glutamate decarboxylase 1
 UniProt Synonym Protein Names   
67 kDa glutamic acid decarboxylase; GAD-67; Glutamate decarboxylase 67 kDa isoform
 Protein Family    Glutamate decarboxylase
 UniProt Gene Name    GAD1 [Similar Products]
 UniProt Synonym Gene Names    GAD; GAD67; GAD-67 [Similar Products]
 UniProt Entry Name    DCE1_HUMAN
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 NCBI Summary for GAD1    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GAD1    GAD67: Catalyzes the production of GABA. Homodimer. Isoform 3 is expressed in pancreatic islets, testis, adrenal cortex, and perhaps other endocrine tissues, but not in brain. Belongs to the group II decarboxylase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Mitochondrial; Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; Lyase; EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - alanine, aspartate and glutamate

Chromosomal Location of Human Ortholog: 2q31

Cellular Component: intracellular; plasma membrane; presynaptic active zone; vesicle membrane

Molecular Function: glutamate binding; glutamate decarboxylase activity; protein binding; protein heterodimerization activity; protein N-terminus binding; pyridoxal phosphate binding

Biological Process: gamma-aminobutyric acid biosynthetic process; glutamate catabolic process; glutamate decarboxylation to succinate; neurotransmitter biosynthetic process; neurotransmitter secretion; protein-pyridoxal-5-phosphate linkage; response to drug; synaptic transmission

Disease: Cerebral Palsy, Spastic Quadriplegic, 1
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 Research Articles on GAD1    1. GAD autoantibodies were associated with risk of developing type 1 diabetes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-GAD1 antibodyDiseases associated with anti-GAD1 antibody
 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >66 publications with GAD1 and Mental Disorders
 Schizophrenia Antibodies  >50 publications with GAD1 and Schizophrenia
 Nervous System Diseases Antibodies  >31 publications with GAD1 and Nervous System Diseases
 Brain Diseases Antibodies  >26 publications with GAD1 and Brain Diseases
 Disease Models, Animal Antibodies  >19 publications with GAD1 and Disease Models, Animal
 Seizures Antibodies  >9 publications with GAD1 and Seizures
 Anxiety Disorders Antibodies  >9 publications with GAD1 and Anxiety Disorders
 Depressive Disorder Antibodies  >8 publications with GAD1 and Depressive Disorder
 Autistic Disorder Antibodies  >6 publications with GAD1 and Autistic Disorder
 Cognition Disorders Antibodies  >5 publications with GAD1 and Cognition Disorders
Organs/Tissues associated with anti-GAD1 antibody
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >120 publications with GAD1 and Brain
 Eye Antibodies  >3 publications with GAD1 and Eye
 Bone Antibodies  >2 publications with GAD1 and Bone
 Embryonic Tissue Antibodies  >2 publications with GAD1 and Embryonic Tissue
 Liver Antibodies  >2 publications with GAD1 and Liver
 Muscle Antibodies  >2 publications with GAD1 and Muscle
 Kidney Antibodies  >2 publications with GAD1 and Kidney
 Thymus Antibodies  >1 publications with GAD1 and Thymus
 Uncharacterized Tissue Antibodies  >1 publications with GAD1 and Uncharacterized Tissue
 Intestine Antibodies  >1 publications with GAD1 and Intestine
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