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anti-GAPDH antibody :: Rabbit GAPDH Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS440117 anti-GAPDH antibody
Unit / Price
0.2 mg  /  $240 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GAPDH, Polyclonal Antibody

 Also Known As   

GAPDH (C17) Antibody

 Product Gene Name   

anti-GAPDH antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for P04797
 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Mouse, rat, human, rabbit, chicken, dog, pig, cat
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 Specificity    Recognizes mouse, rat, human, rabbit, chicken, dog, pig, and cat GAPDH by western blotting.
 Form/Format    200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
 Concentration    0.200 mg/ml (lot specific)
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 Origin    GAPDH (C17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the carboxy terminus of human GAPDH.
 Immunogen    A synthetic peptide mapping near the carboxy terminus of human GAPDH.
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 Preparation and Storage    Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
 Other Notes    Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GAPDH antibody

   Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. In more recent studies GAPDH has been shown to be involved in membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. GAPDH has a molecular mass of 37 kDa and its expression is upregulated in prostate, liver, and lung cancers (1-3).
 Applications Tested/Suitable for anti-GAPDH antibody   

Western Blot (WB)

 Application Notes for anti-GAPDH antibody    Recommended western blotting starting dilution 1:400.
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    122065190
 NCBI GeneID    24383
 NCBI Accession #    P04797.3 [Other Products]
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 UniProt Primary Accession #    P04797 [Other Products]
 UniProt Secondary Accession #    P09328; Q5M916; Q9QWU4 [Other Products]
 UniProt Related Accession #    P04797 [Other Products]
 Molecular Weight    35,828 Da
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 NCBI Official Full Name    Glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    Gapdh [Similar Products]
 NCBI Official Synonym Symbols   
Gapd; BARS-38
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; peptidyl-cysteine S-nitrosylase GAPDH
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 UniProt Gene Name    Gapdh [Similar Products]
 UniProt Synonym Gene Names    Gapd; GAPDH [Similar Products]
 UniProt Entry Name    G3P_RAT
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 NCBI Summary for GAPDH    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Oxidoreductase; EC 1.2.1.12

Cellular Component: microtubule cytoskeleton; nuclear membrane; mitochondrion; membrane; intracellular membrane-bound organelle; cytoplasm; plasma membrane; lipid particle; nucleus; cytosol; ribonucleoprotein complex; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: regulation of translation; neuron apoptosis; glycolysis; protein stabilization; apoptosis; negative regulation of translation; multicellular organismal development; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis
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Product References and Citations for anti-GAPDH antibody

   1.Meyer-Siegler K, Mauro DJ, Seal G, Wurzer J, deRiel JK, and Sirover MA. 1991. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glcyeraldehyde-3-phosphate dehydrogenase. PNAS USA 88: 8460-8464.2.Rondinelli RH, Epner DE, and Tricoli JV. 1997. Increased glyceraldehydes-3-phosphate dehydrogenase gene expression in late pathological stage human prostate cancer. Prostate Cancer Prostatic Dis. 2: 66-72.3.Tarbe N, Evtimova V, Burtscher H, Jarsch M, Alves F, and Weidle UH. 2001. Transcriptional profiling of cell lines derived from an orthotopic pancreatic tumor model reveals metastasis-associated genes. Anticancer Res. 5: 3221-3228.
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 Research Articles on GAPDH    1. The nuclear GAPDH forms a protein complex with p53 and enhances p53 expression and phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAPDH antibodyPathways associated with anti-GAPDH antibody
 Reference Product  PubMed Publications
 Pgk1 antibody  >50 publications with GAPDH and Pgk1
 NSE antibody  >13 publications with GAPDH and NSE
 Eno1 antibody  >11 publications with GAPDH and Eno1
 Tpi1 antibody  >7 publications with GAPDH and Tpi1
 Eno2 antibody  >6 publications with GAPDH and Eno2
 Akt1 antibody  >4 publications with GAPDH and Akt1
 Pgk2 antibody  >1 publications with GAPDH and Pgk2
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Diseases associated with anti-GAPDH antibodyOrgans/Tissues associated with anti-GAPDH antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >282 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >254 publications with GAPDH and Cardiovascular Diseases
 Necrosis Antibodies  >210 publications with GAPDH and Necrosis
 Brain Diseases Antibodies  >209 publications with GAPDH and Brain Diseases
 Inflammation Antibodies  >191 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >148 publications with GAPDH and Heart Diseases
 Liver Diseases Antibodies  >138 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >95 publications with GAPDH and Kidney Diseases
 Liver Neoplasms Antibodies  >78 publications with GAPDH and Liver Neoplasms
 Lung Neoplasms Antibodies  >73 publications with GAPDH and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >539 publications with GAPDH and Muscle
 Brain Antibodies  >490 publications with GAPDH and Brain
 Liver Antibodies  >399 publications with GAPDH and Liver
 Heart Antibodies  >294 publications with GAPDH and Heart
 Kidney Antibodies  >254 publications with GAPDH and Kidney
 Artery Antibodies  >126 publications with GAPDH and Artery
 Embryonic Tissue Antibodies  >121 publications with GAPDH and Embryonic Tissue
 Eye Antibodies  >90 publications with GAPDH and Eye
 Connective Tissue Antibodies  >88 publications with GAPDH and Connective Tissue
 Colon Antibodies  >73 publications with GAPDH and Colon
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