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anti-GAPDH antibody :: Rabbit GAPDH Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS857640
Western Blot
Unit / Price
0.05 mg  /  $175 +1 FREE 8GB USB
0.1 mg  /  $215 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GAPDH, Polyclonal Antibody

 Also Known As   

GAPDH Polyclonal Antibody

 Product Gene Name   

anti-GAPDH antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
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 Form/Format    Rabbit polyclonal (IgG)
1mg/ml in PBS, pH 7.4 with 50% glycerol, 0.05% sodium azide. Frozen liquid.
 Concentration    50ug/50ul (lot specific)
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 Immunogen    Purified recombinant human GAPDH.
 Pathway    Internal control
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 Preparation and Storage    Stable for 1 year at -20 degree C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to opening the cap. Aliquot to avoid repeated freezing and thawing. Aliquot will be stable at 4 degree C for 3 months.
 Other Notes    Small volumes of anti-GAPDH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-GAPDH antibody    Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells; Autophagy antibody
 Applications Tested/Suitable for anti-GAPDH antibody   

Western Blot (WB)

 Application Notes for anti-GAPDH antibody    Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows: Western blot at dilution of 1:1000-4000, Immunofluorescence staining at 1:100-400 dilutions, IHC at 1:100-400 dilutions. It is very useful as a loading control for quantitative western blotting. The antibody recognizes a 37kDa band in a Western Blot analysis.
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 Western Blot of anti-GAPDH antibody    Western blot analysis of GAPDH expression in 293A (A) and Hela (B) whole cell lysates and mouse kidney tissue extract (C) using GAPDH polyclonal antibody.
anti-GAPDH antibody Western Blot (WB) image
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NCBI/Uniprot data below describe general gene information for GAPDH. It may not necessarily be applicable to this product.
 NCBI GI #    122065190
 NCBI GeneID    24383
 NCBI Accession #    P04797.3 [Other Products]
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 UniProt Secondary Accession #    P09328; Q5M916; Q9QWU4 [Other Products]
 UniProt Related Accession #    P04797 [Other Products]
 Molecular Weight    35,828 Da
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 NCBI Official Full Name    Glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Synonym Full Names    glyceraldehyde-3-phosphate dehydrogenase
 NCBI Official Symbol    Gapdh [Similar Products]
 NCBI Official Synonym Symbols   
Gapd; BARS-38
[Similar Products]
 NCBI Protein Information    glyceraldehyde-3-phosphate dehydrogenase; peptidyl-cysteine S-nitrosylase GAPDH; 38 kDa BFA-dependent ADP-ribosylation substrate
 UniProt Protein Name    Glyceraldehyde-3-phosphate dehydrogenase
 UniProt Synonym Protein Names   
38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
 UniProt Gene Name    Gapdh [Similar Products]
 UniProt Synonym Gene Names    Gapd; GAPDH [Similar Products]
 UniProt Entry Name    G3P_RAT
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 NCBI Summary for GAPDH    This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GAPDH    GAPDH: a multifunctional enzyme with both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities. A key glycolytic enzyme that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. An important enzyme for energy metabolism, and the production of ATP and pyruvate through anaerobic glycolysis in the cytoplasm. Additionally, it participates in apoptosis, membrane trafficking, iron metabolism, nuclear activities and receptor mediated cell signaling. Its subcellular localization changes reflecting its multiple activities. Is cytosolic, but is also localized in the membrane, the nucleus, polysomes, the ER and the Golgi. Participates in transcription, RNA transport, DNA replication and apoptosis. S-nitrosylation on Cys-152 following apoptotic stimulates its interaction with SIAH2, which in turn moderates its translocation into the nucleus. Mediates cysteine S-nitrosylation of nuclear target proteins including SIRT1, HDAC2 and DNA-PK. Deregulated in lung cancer, renal cancer, breast cancer, gastric cancer, glioma, liver cancer, colorectal cancer, melanoma, prostatic cancer, pancreatic cancer and bladder cancer. Its increased expression and enzymatic activity is associated with cell proliferation and tumorigenesis, Oxidative stress impairs GAPDH catalytic activity and leads to cellular aging and apoptosis. In experimental animal models, injection of GAPDH antagonists induces apoptosis and blocks Hep3B tumor progression, suggesting a therapeutic potential of targeting GAPDH in human hepatocellular carcinoma

Protein type: Oxidoreductase; Carbohydrate Metabolism - glycolysis and gluconeogenesis; EC 1.2.1.12

Cellular Component: microtubule cytoskeleton; nuclear membrane; mitochondrion; intracellular membrane-bound organelle; membrane; cytoplasm; plasma membrane; lipid particle; nucleus; ribonucleoprotein complex; cytosol; vesicle

Molecular Function: identical protein binding; protein binding; microtubule binding; glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity; NADP binding; NAD binding

Biological Process: regulation of translation; neuron apoptosis; glycolysis; protein stabilization; apoptosis; multicellular organismal development; negative regulation of translation; carbohydrate metabolic process; microtubule cytoskeleton organization and biogenesis; gluconeogenesis
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Product References and Citations for anti-GAPDH antibody

   Cummings J, et al. (2005) Br J Cancer; 92 (3): 532-538.
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 Research Articles on GAPDH    1. The nuclear GAPDH forms a protein complex with p53 and enhances p53 expression and phosphorylation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAPDH antibodyPathways associated with anti-GAPDH antibody
 Reference Product  PubMed Publications
 Actb antibody  >229 publications with GAPDH and Actb
 Pgk1 antibody  >45 publications with GAPDH and Pgk1
 Egfr antibody  >15 publications with GAPDH and Egfr
 Tpi1 antibody  >7 publications with GAPDH and Tpi1
 Akt1 antibody  >3 publications with GAPDH and Akt1
 Il2 antibody  >2 publications with GAPDH and Il2
 Fn1 antibody  >1 publications with GAPDH and Fn1
 Products by Pathway  Pathway Diagram
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 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Biosynthesis Of Amino Acids Pathway antibodies  Biosynthesis Of Amino Acids Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies  Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
Diseases associated with anti-GAPDH antibodyOrgans/Tissues associated with anti-GAPDH antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >273 publications with GAPDH and Nervous System Diseases
 Cardiovascular Diseases Antibodies  >246 publications with GAPDH and Cardiovascular Diseases
 Brain Diseases Antibodies  >202 publications with GAPDH and Brain Diseases
 Necrosis Antibodies  >201 publications with GAPDH and Necrosis
 Inflammation Antibodies  >182 publications with GAPDH and Inflammation
 Heart Diseases Antibodies  >146 publications with GAPDH and Heart Diseases
 Liver Diseases Antibodies  >135 publications with GAPDH and Liver Diseases
 Kidney Diseases Antibodies  >90 publications with GAPDH and Kidney Diseases
 Liver Neoplasms Antibodies  >75 publications with GAPDH and Liver Neoplasms
 Lung Neoplasms Antibodies  >72 publications with GAPDH and Lung Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >524 publications with GAPDH and Muscle
 Brain Antibodies  >475 publications with GAPDH and Brain
 Liver Antibodies  >383 publications with GAPDH and Liver
 Heart Antibodies  >284 publications with GAPDH and Heart
 Kidney Antibodies  >245 publications with GAPDH and Kidney
 Artery Antibodies  >122 publications with GAPDH and Artery
 Embryonic Tissue Antibodies  >121 publications with GAPDH and Embryonic Tissue
 Eye Antibodies  >87 publications with GAPDH and Eye
 Connective Tissue Antibodies  >84 publications with GAPDH and Connective Tissue
 Colon Antibodies  >67 publications with GAPDH and Colon
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