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anti-GFI1B antibody :: Rabbit anti-Human, mouse GFI1B Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9212717
Western Blot (WB)
Unit / Price
0.08 mL  /  $150 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GFI1B, Polyclonal Antibody

★Popular Item★
 Also Known As   

GFI1B Antibody (C-term)

 Product Synonym Names    Zinc finger protein Gfi-1b; Growth factor independent protein 1B; Potential regulator of CDKN1A translocated in CML; GFI1B
 Product Gene Name   

anti-GFI1B antibody

[Similar Products]
 Antibody/Peptide Pairs    GFI1B peptide (MBS9229077) is used for blocking the activity of GFI1B antibody (MBS9212717)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    302-330
 OMIM    187900
 3D Structure    ModBase 3D Structure for Q5VTD9
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human, mouse
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 Specificity    This GFI1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human GFI1B.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
 Concentration    Vial Concentration: 0.5 (lot specific)
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 Antigen Type    Synthetic Peptide
 Crown Antibody    Yes
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-GFI1B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GFI1B antibody

   Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down-regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor CDKN1A/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma-satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation.
 Product Categories/Family for anti-GFI1B antibody    Crown Antibodies; Cardiovascular
 Applications Tested/Suitable for anti-GFI1B antibody   

Western Blot (WB), ELISA (EIA)

 Application Notes for anti-GFI1B antibody    WB~~1:1000
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 Western Blot (WB) of anti-GFI1B antibody    GFI1B Antibody (C-term) western blot analysis in HL-60,K562,U266
cell line and mouse spleen lysates (35ug/lane).This demonstrates the GFI1B antibody detected the GFI1B protein (arrow).
anti-GFI1B antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for GFI1B. It may not necessarily be applicable to this product.
 NCBI GI #    205277345
 NCBI GeneID    8328
 NCBI Accession #    NP_001128503.1 [Other Products]
 NCBI Related Accession #    NP_004179.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_001135031.1 [Other Products]
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 UniProt Primary Accession #    Q5VTD9 [Other Products]
 UniProt Secondary Accession #    O95270; Q5VTD8; Q6FHZ2; Q6T888 [Other Products]
 UniProt Related Accession #    Q5VTD9 [Other Products]
 Molecular Weight    37492
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 NCBI Official Full Name    zinc finger protein Gfi-1b isoform 2
 NCBI Official Synonym Full Names    growth factor independent 1B transcription repressor
 NCBI Official Symbol    GFI1B [Similar Products]
 NCBI Official Synonym Symbols   
BDPLT17; ZNF163B
[Similar Products]
 NCBI Protein Information    zinc finger protein Gfi-1b
 UniProt Protein Name    Zinc finger protein Gfi-1b
 UniProt Synonym Protein Names   
Growth factor independent protein 1B; Potential regulator of CDKN1A translocated in CML
 Protein Family    Zinc finger protein
 UniProt Gene Name    GFI1B [Similar Products]
 UniProt Entry Name    GFI1B_HUMAN
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 NCBI Summary for GFI1B    This gene encodes a zinc-finger containing transcriptional regulator that is primarily expressed in cells of hematopoietic lineage. The encoded protein complexes with numerous other transcriptional regulatory proteins including GATA-1, runt-related transcription factor 1 and histone deacetylases to control expression of genes involved in the development and maturation of erythrocytes and megakaryocytes. Mutations in this gene are the cause of the autosomal dominant platelet disorder, platelet-type bleeding disorder-17. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Aug 2014]
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 UniProt Comments for GFI1B    GFI1B: Essential proto-oncogenic transcriptional regulator necessary for development and differentiation of erythroid and megakaryocytic lineages. Component of a RCOR-GFI-KDM1A-HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development and controls hematopoietic differentiation. Transcriptional repressor or activator depending on both promoter and cell type context; represses promoter activity of SOCS1 and SOCS3 and thus, may regulate cytokine signaling pathways. Cooperates with GATA1 to repress target gene transcription, such as the apoptosis regulator BCL2L1; GFI1B silencing in leukemic cell lines markedly increase apoptosis rate. Inhibits down- regulation of MYC and MYB as well as the cyclin-dependent kinase inhibitor p21Cip1/P21WAF1 in IL6-treated myelomonocytic cells. Represses expression of GATA3 in T-cell lymphomas and inhibits GATA1-mediated transcription; as GATA1 also mediates erythroid GFI1B transcription, both GATA1 and GFI1B participate in a feedback regulatory pathway controlling the expression of GFI1B gene in erythroid cells. Suppresses GATA1-mediated stimulation of GFI1B promoter through protein interaction. Binds to gamma- satellite DNA and to its own promoter, auto-repressing its own expression. Alters histone methylation by recruiting histone methyltransferase to target genes promoters. Plays a role in heterochromatin formation. Component of a RCOR-GFI-KDM1A-HDAC complex. Interacts directly with RCOR1, KDM1A and HDAC2. Forms a complex with GATA1. Interacts with histone methyltransferases EHMT2 and SUV39H1. Interacts with ARIH2 (via RING-type 2). Interacts with RUNX1T1. By GATA1 which binds to GFI1B promoter in cooperation with the transcription factor NFYA. Target gene of transcription factor E2-alpha/TCF3 that promotes growth arrest and apoptosis in lymphomas. Expressed in bone marrow and fetal liver, but also detectable in fetal spleen, fetal thymus, and testes. Detected in hematopoietic stem cells, erythroblasts, and megakaryocytes. Overexpressed in bone marrow of patients with erythroleukemia and megakaryocytic leukemia as well as in their corresponding leukemic cell lines, and markedly repressed in severe aplastic anemia (SAA). 2 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein; Oncoprotein

Chromosomal Location of Human Ortholog: 9q34.13

Cellular Component: transcription factor complex; nuclear matrix; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding

Biological Process: transcription from RNA polymerase II promoter; cell proliferation; regulation of histone H3-K4 methylation; hemopoiesis; negative regulation of transcription from RNA polymerase II promoter; chromatin modification

Disease: Bleeding Disorder, Platelet-type, 17
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Product References and Citations for anti-GFI1B antibody

   Roedel B., et al. Genomics 54:580-582(1998).
Huang D.Y., et al. Nucleic Acids Res. 32:3935-3946(2004).
Halleck A., et al. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Humphray S.J., et al. Nature 429:369-374(2004).
Mural R.J., et al. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
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 Research Articles on GFI1B    1. a mutation in GFI1B causes a platelet disorder; GFI1B has a role a critical regulator of platelet shape, number, and function
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GFI1B antibodyDiseases associated with anti-GFI1B antibody
 Reference Product  PubMed Publications
 GATA1 antibody  >13 publications with GFI1B and GATA1
 RCOR1 antibody  >5 publications with GFI1B and RCOR1
 RCOR2 antibody  >4 publications with GFI1B and RCOR2
 KDM1A antibody  >3 publications with GFI1B and KDM1A
 GATA3 antibody  >2 publications with GFI1B and GATA3
 RCOR3 antibody  >1 publications with GFI1B and RCOR3
 MEGF8 antibody  >1 publications with GFI1B and MEGF8
 HMG20B antibody  >1 publications with GFI1B and HMG20B
 Disease Name  Pubmed Publications
 Immune System Diseases Antibodies  >7 publications with GFI1B and Immune System Diseases
 Cell Transformation, Neoplastic Antibodies  >4 publications with GFI1B and Cell Transformation, Neoplastic
 Thrombosis Antibodies  >1 publications with GFI1B and Thrombosis
 Heart Defects, Congenital Antibodies  >1 publications with GFI1B and Heart Defects, Congenital
 Brain Diseases Antibodies  >1 publications with GFI1B and Brain Diseases
 Abnormalities, Drug-Induced Antibodies  >1 publications with GFI1B and Abnormalities, Drug-Induced
Organs/Tissues associated with anti-GFI1B antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Marrow Antibodies  >12 publications with GFI1B and Bone Marrow
 Liver Antibodies  >8 publications with GFI1B and Liver
 Heart Antibodies  >3 publications with GFI1B and Heart
 Spleen Antibodies  >3 publications with GFI1B and Spleen
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