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anti-GLI3 antibody :: Goat GLI3 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS420368 anti-GLI3 antibody
Unit / Price
0.1 mg  /  $225 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GLI3, Polyclonal Antibody

 Also Known As   

Goat anti-GLI3 Antibody

 Product Synonym Names    GLI3; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome); ACLS; GCPS; PAP-A; PAPA; PAPA1; PAPB; PHS; PPDIV; DNA-binding protein; GLI-Kruppel family member GLI3; oncogene GLI3; zinc finger protein GLI3; GLI3 antibody; GLI-Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome) antibody; ACLS antibody; GCPS antibody; PAP-A antibody; PAPA antibody; PAPA1 antibody; PAPB antibody; PHS antibody; PPDIV antibody; DNA-binding protein antibody; GLI-Kruppel family member GLI3 antibody; oncogene GLI3 antibody; zinc finger protein GLI3 antibody
 Product Gene Name   

anti-GLI3 antibody

[Similar Products]
 Antibody/Peptide Pairs    GLI3 peptide (MBS426356) is used for blocking the activity of GLI3 antibody (MBS420368)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    HNKRSKIKPD ED
 OMIM    146510
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Expected from sequence similarity: Human, Mouse, Rat, Dog
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence C-HNKRSKIKPDED, from the internal region of the protein sequence according to NP_000159.2.
 Epitope    Internal region
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-GLI3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-GLI3 antibody   

Peptide ELISA (EIA)

 Application Notes for anti-GLI3 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments in Human Brain, Lung, Placenta and Testes lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml).
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NCBI/Uniprot data below describe general gene information for GLI3. It may not necessarily be applicable to this product.
 NCBI GI #    13518032
 NCBI GeneID    2737
 NCBI Accession #    NP_000159.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000168.2 [Other Products]
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 UniProt Secondary Accession #    O75219; Q17RW4; Q75MT0; Q75MU9; Q9UDT5; Q9UJ39; A4D1W1 [Other Products]
 UniProt Related Accession #    P10071 [Other Products]
 Molecular Weight    169,863 Da
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 NCBI Official Full Name    GLI-Kruppel family member GLI3
 NCBI Official Synonym Full Names    GLI family zinc finger 3
 NCBI Official Symbol    GLI3 [Similar Products]
 NCBI Official Synonym Symbols   
PHS; ACLS; GCPS; PAPA; PAPB; PAP-A; PAPA1; PPDIV; GLI3FL; GLI3-190
[Similar Products]
 NCBI Protein Information    transcriptional activator GLI3
 UniProt Protein Name    Transcriptional activator GLI3
 UniProt Synonym Protein Names   
GLI3 form of 190 kDa; GLI3-190
 Protein Family    Zinc finger protein
 UniProt Gene Name    GLI3 [Similar Products]
 UniProt Synonym Gene Names    GLI3-190; GLI3FL; GLI3-83 [Similar Products]
 UniProt Entry Name    GLI3_HUMAN
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 NCBI Summary for GLI3    This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B. [provided by RefSeq, Jul 2008]
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 UniProt Comments for GLI3    GLI3: Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. Defects in GLI3 are the cause of Greig cephalo-poly- syndactyly syndrome (GCPS). GCPS is an autosomal dominant disorder affecting limb and craniofacial development. It is characterized by pre- and postaxial polydactyly, syndactyly of fingers and toes, macrocephaly and hypertelorism. Defects in GLI3 are a cause of Pallister-Hall syndrome (PHS). PHS is characterized by a wide range of clinical manifestations. It mainly associates central or postaxial polydactyly, syndactyly, and hypothalamic hamartoma. Malformations are frequent in the viscera, e.g. anal atresia, bifid uvula, congenital heart malformations, pulmonary or renal dysplasia. It is an autosomal dominant disorder. Defects in GLI3 are a cause of polydactyly postaxial type A1 (PAPA1). A trait characterized by an extra digit in the ulnar and/or fibular side of the upper and/or lower extremities. The extra digit is well formed and articulates with the fifth, or extra, metacarpal/metatarsal, and thus it is usually functional. Defects in GLI3 are a cause of polydactyly postaxial type B polydactyly (PAPB). A trait characterized by an extra digit in the ulnar and/or fibular side of the upper and/or lower extremities. The extra digit is not well formed and is frequently in the form of a skin. Defects in GLI3 are a cause of polydactyly preaxial type 4 (POP4). Polydactyly preaxial type 4 (i.e. polydactyly on the radial/tibial side of the hand/foot) covers a heterogeneous group of entities. In preaxial polydactyly type IV, the thumb shows only the mildest degree of duplication, and syndactyly of various degrees affects fingers 3 and 4. Belongs to the GLI C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; Transcription factor

Chromosomal Location of Human Ortholog: 7p13

Cellular Component: axoneme; cilium; cytoplasm; cytosol; nuclear speck; nucleoplasm; nucleus; Srb-mediator complex; transcriptional repressor complex

Molecular Function: beta-catenin binding; chromatin binding; histone acetyltransferase binding; histone deacetylase binding; metal ion binding; protein binding; transcription factor activity

Biological Process: anterior/posterior pattern formation; axon guidance; camera-type eye morphogenesis; developmental growth; embryonic digestive tract morphogenesis; embryonic digit morphogenesis; embryonic gut development; embryonic skeletal morphogenesis; forebrain dorsal/ventral pattern formation; heart development; hindgut morphogenesis; hippocampus development; in utero embryonic development; lateral ganglionic eminence cell proliferation; layer formation in the cerebral cortex; limb morphogenesis; lung development; melanocyte differentiation; metanephros development; negative regulation of alpha-beta T cell differentiation; negative regulation of apoptosis; negative regulation of cell proliferation; negative regulation of neuron differentiation; negative regulation of smoothened signaling pathway; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; negative thymic T cell selection; nose morphogenesis; odontogenesis of dentine-containing teeth; oligodendrocyte differentiation; optic nerve morphogenesis; palate development; positive regulation of alpha-beta T cell differentiation; positive regulation of chondrocyte differentiation; positive regulation of neuroblast proliferation; positive regulation of osteoblast differentiation; positive regulation of protein import into nucleus; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein processing; proximal/distal pattern formation; radial glial cell differentiation in the forebrain; response to estrogen stimulus; smoothened signaling pathway; smoothened signaling pathway involved in spinal cord motor neuron cell fate specification; smoothened signaling pathway involved in ventral spinal cord interneuron specification; T cell differentiation in the thymus; tongue development; transcription from RNA polymerase II promoter; ureteric bud branching; wound healing

Disease: Greig Cephalopolysyndactyly Syndrome; Hypothalamic Hamartomas; Pallister-hall Syndrome; Polydactyly, Postaxial, Type A1; Polydactyly, Preaxial Iv; Tracheoesophageal Fistula With Or Without Esophageal Atresia
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 Research Articles on GLI3    1. We report on a patient with GCPS caused by a novel GLI3 mutation.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GLI3 antibodyPathways associated with anti-GLI3 antibody
 Reference Product  PubMed Publications
 SHH antibody  >342 publications with GLI3 and SHH
 SMO antibody  >85 publications with GLI3 and SMO
 PTCH1 antibody  >68 publications with GLI3 and PTCH1
 IHH antibody  >47 publications with GLI3 and IHH
 SUFU antibody  >39 publications with GLI3 and SUFU
 SPOP antibody  >7 publications with GLI3 and SPOP
 GSK3B antibody  >3 publications with GLI3 and GSK3B
 BTRC antibody  >2 publications with GLI3 and BTRC
 STK36 antibody  >2 publications with GLI3 and STK36
 Products by Pathway  Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Basal Cell Carcinoma Pathway antibodies  Basal Cell Carcinoma Pathway Diagram
 Endochondral Ossification Pathway antibodies  Endochondral Ossification Pathway Diagram
 GLI Proteins Bind Promoters Of Hh Responsive Genes To Promote Transcription Pathway antibodies  GLI Proteins Bind Promoters Of Hh Responsive Genes To Promote Transcription Pathway Diagram
 GLI3 Is Processed To GLI3R By The Proteasome Pathway antibodies  GLI3 Is Processed To GLI3R By The Proteasome Pathway Diagram
 Hedgehog 'off' State Pathway antibodies  Hedgehog 'off' State Pathway Diagram
 Hedgehog 'on' State Pathway antibodies  Hedgehog 'on' State Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
 Hedgehog Signaling Pathway antibodies  Hedgehog Signaling Pathway Diagram
Diseases associated with anti-GLI3 antibodyOrgans/Tissues associated with anti-GLI3 antibody
 Disease Name  Pubmed Publications
 Polydactyly Antibodies  >142 publications with GLI3 and Polydactyly
 Greig cephalopolysyndactyly syndrome Antibodies  >58 publications with GLI3 and Greig cephalopolysyndactyly syndrome
 Pallister-Hall Syndrome Antibodies  >57 publications with GLI3 and Pallister-Hall Syndrome
 Carcinoma Antibodies  >45 publications with GLI3 and Carcinoma
 Nervous System Malformations Antibodies  >34 publications with GLI3 and Nervous System Malformations
 Neural Tube Defects Antibodies  >31 publications with GLI3 and Neural Tube Defects
 Disease Models, Animal Antibodies  >29 publications with GLI3 and Disease Models, Animal
 Chromosome Aberrations Antibodies  >25 publications with GLI3 and Chromosome Aberrations
 Adenocarcinoma Antibodies  >16 publications with GLI3 and Adenocarcinoma
 Cardiovascular Diseases Antibodies  >13 publications with GLI3 and Cardiovascular Diseases
 Organ/Tissue Name  Pubmed Publications
 Embryonic Tissue Antibodies  >228 publications with GLI3 and Embryonic Tissue
 Brain Antibodies  >123 publications with GLI3 and Brain
 Bone Antibodies  >109 publications with GLI3 and Bone
 Eye Antibodies  >37 publications with GLI3 and Eye
 Lung Antibodies  >29 publications with GLI3 and Lung
 Muscle Antibodies  >27 publications with GLI3 and Muscle
 Connective Tissue Antibodies  >27 publications with GLI3 and Connective Tissue
 Heart Antibodies  >25 publications with GLI3 and Heart
 Blood Antibodies  >24 publications with GLI3 and Blood
 Skin Antibodies  >23 publications with GLI3 and Skin
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