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anti-MASTL antibody :: GW Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS8225930
Unit / Price
0.03 mL  /  $155 +1 FREE 8GB USB
0.1 mL  /  $220 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

GW (MASTL), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-GW Antibody

 Product Synonym Names    GW; GWL; THC2; Serine/threonine-protein kinase greatwall; GW; GWL; hGWL; Microtubule-associated serine/threonine-protein kinase-like; MAST-L
 Product Gene Name   

anti-MASTL antibody

[Similar Products]
 Antibody/Peptide Pairs    GW peptide (MBS8202921) is used for blocking the activity of GW antibody (MBS8225930)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    188000
 3D Structure    ModBase 3D Structure for Q96GX5
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 Clonality    Polyclonal
 Species Reactivity    Human, Mouse, Bovine, Chicken, Dog
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 Specificity    Recognizes endogenous levels of GW protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human GW. The exact sequence is proprietary.
 Source    Rabbit
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-MASTL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MASTL antibody

   Rabbit polyclonal antibody to GW
 Applications Tested/Suitable for anti-MASTL antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-MASTL antibody    WB (1/500 - 1/1000), IHC (1/100 - 1/200)
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 Western Blot (WB) of anti-MASTL antibody    Western blot analysis of GW expression in HeLa (A), U2OS (B) whole cell lysates.
anti-MASTL antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-MASTL antibody    Immunohistochemical analysis of GW staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugad compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-MASTL antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for MASTL. It may not necessarily be applicable to this product.
 NCBI GI #    288806587
 NCBI GeneID    84930
 NCBI Accession #    NP_001165774.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001172303.1 [Other Products]
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 UniProt Primary Accession #    Q96GX5 [Other Products]
 UniProt Secondary Accession #    Q5T8D5; Q5T8D7; Q8NCD6; Q96SJ5 [Other Products]
 UniProt Related Accession #    Q96GX5 [Other Products]
 Molecular Weight    97,191 Da
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 NCBI Official Full Name    serine/threonine-protein kinase greatwall isoform 1
 NCBI Official Synonym Full Names    microtubule associated serine/threonine kinase-like
 NCBI Official Symbol    MASTL [Similar Products]
 NCBI Official Synonym Symbols   
GW; GWL; THC2; hGWL; MAST-L; GREATWALL
[Similar Products]
 NCBI Protein Information    serine/threonine-protein kinase greatwall
 UniProt Protein Name    Serine/threonine-protein kinase greatwall
 UniProt Synonym Protein Names   
Microtubule-associated serine/threonine-protein kinase-like; MAST-L
 Protein Family    Serine/threonine-protein kinase greatwall
 UniProt Gene Name    MASTL [Similar Products]
 UniProt Synonym Gene Names    GW; GWL; THC2; GW; GWL; hGWL; MAST-L [Similar Products]
 UniProt Entry Name    GWL_HUMAN
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 NCBI Summary for MASTL    This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]
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 UniProt Comments for MASTL    MASTL: Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. Defects in MASTL are the cause of thrombocytopenia type 2 (THC2). Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Protein kinase, AGC; Kinase, protein; AGC group; MAST family

Chromosomal Location of Human Ortholog: 10p12.1

Cellular Component: nucleoplasm; centrosome; cytoplasm; nucleus; cleavage furrow

Molecular Function: protein serine/threonine kinase activity; protein phosphatase 2A binding; kinase activity; ATP binding

Biological Process: mitosis; cell division; regulation of cell cycle; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein phosphatase type 2A activity; protein amino acid phosphorylation; response to DNA damage stimulus

Disease: Thrombocytopenia 2
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 Research Articles on MASTL    1. data demonstrate that GWL acts in a pathway with PP2A which is essential for prophase I exit and metaphase I microtubule assembly in mouse oocytes.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MASTL antibodyPathways associated with anti-MASTL antibody
 Reference Product  PubMed Publications
 CDK1 antibody  >12 publications with MASTL and CDK1
 ENSA antibody  >4 publications with MASTL and ENSA
 CCNB1 antibody  >2 publications with MASTL and CCNB1
 ARPP19 antibody  >2 publications with MASTL and ARPP19
 PPP2R2A antibody  >1 publications with MASTL and PPP2R2A
 PPP2R2D antibody  >1 publications with MASTL and PPP2R2D
 Products by Pathway  Pathway Diagram
 Cell Cycle Pathway antibodies  Cell Cycle Pathway Diagram
 Cell Cycle, Mitotic Pathway antibodies  Cell Cycle, Mitotic Pathway Diagram
 M Phase Pathway antibodies  M Phase Pathway Diagram
 MASTL Facilitates Mitotic Progression Pathway antibodies  MASTL Facilitates Mitotic Progression Pathway Diagram
 Mitotic Prophase Pathway antibodies  Mitotic Prophase Pathway Diagram
Diseases associated with anti-MASTL antibodyOrgans/Tissues associated with anti-MASTL antibody
 Disease Name  Pubmed Publications
 Disease Models, Animal Antibodies  >1 publications with MASTL and Disease Models, Animal
 Breast Neoplasms Antibodies  >1 publications with MASTL and Breast Neoplasms
 Liver Diseases Antibodies  >1 publications with MASTL and Liver Diseases
 Neoplasms, Experimental Antibodies  >1 publications with MASTL and Neoplasms, Experimental
 Liver Neoplasms Antibodies  >1 publications with MASTL and Liver Neoplasms
 Cell Transformation, Neoplastic Antibodies  >1 publications with MASTL and Cell Transformation, Neoplastic
 THROMBOCYTOPENIA 2 Antibodies  >1 publications with MASTL and THROMBOCYTOPENIA 2
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >2 publications with MASTL and Blood
 Lung Antibodies  >1 publications with MASTL and Lung
 Bone Antibodies  >1 publications with MASTL and Bone
 Bone Marrow Antibodies  >1 publications with MASTL and Bone Marrow
 Kidney Antibodies  >1 publications with MASTL and Kidney
 Liver Antibodies  >1 publications with MASTL and Liver
 Intestine Antibodies  >1 publications with MASTL and Intestine
 Testis Antibodies  >1 publications with MASTL and Testis
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