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anti-GPI antibody :: Rabbit anti-Human Glucose-6-phosphate isomerase Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS7001878
Unit / Price
0.05 mg  /  $160 +1 FREE 8GB USB
0.1 mg  /  $235 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glucose-6-phosphate isomerase (GPI), Polyclonal Antibody

★Popular Item★
 Also Known As   

Rabbit anti-human Glucose-6-phosphate isomerase polyclonal Antibody

 Product Synonym Names    Autocrine motility factor; Phosphoglucose isomerase; Phosphohexose isomerase; Sperm antigen 36
 Product Gene Name   

anti-GPI antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    172400
 3D Structure    ModBase 3D Structure for P06744
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Caprylic Acid Ammonium Sulfate Precipitation purified
 Form/Format    Liquid
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 Immunogen    Recombinant human Glucose-6-phosphate isomerase
 Conjugation    Non-conjugated
 Storage Buffer    Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
 Santa Cruz Alternative    Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-30392 / sc-33777
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery, aliquot and store at -20 degree C or -80 degree C.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-GPI antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GPI antibody

   Besides it's role as a glycolytic enzyme, mammalian GPI can function as a tumor-secreted cytokine and an angiogenic factor (AMF) that stimulates endothelial cell motility. GPI is also a neurotrophic factor (Neuroleukin) for spinal and sensory neurons.

This gene belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. This protein is a dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. The protein functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.
 Product Categories/Family for anti-GPI antibody    Signal Transduction
 Applications Tested/Suitable for anti-GPI antibody   

ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-GPI antibody    Recommended dilution: IHC: 1:20-1:200
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 Immunohistochemistry (IHC) of anti-GPI antibody    Immunohistochemistry of paraffin-embeded human kidney using MBS7001878 at dilution of 1:100
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-GPI antibody    Western Blot
Positive WB detected in: PC3 whole cell lysate,Hela whole cell lysate,Rat brain tissue,Mouse heart tissue,Mouse liver tissue,Mouse kidney tissue,Mouse brain tissue
All lanes: GPI antibody at 2.7ug/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 64 KDa
Observed band size: 64 KDa

anti-GPI antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-GPI antibody    Immunohistochemistry of paraffin-embedded human colon cancer using MBS7001878 at dilution of 1:100
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
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 Immunohistochemistry (IHC) of anti-GPI antibody    Immunohistochemistry of paraffin-embedded human endometrial cancer using MBS7001878 at dilution of 1:100
anti-GPI antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence (IF) of anti-GPI antibody    Immunofluorescent analysis of PC3 cells using MBS7001878 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
anti-GPI antibody Immunofluorescence (IF) image
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 Immunoprecipitation (IP) of anti-GPI antibody    Immunoprecipitating GPI in Hela whole cell lysate
Lane 1: Rabbit monoclonal IgG (1ug)instead of MBS7001878 in Hela whole cell lysate. For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: MBS7001878 (8ug)+ Hela whole cell lysate (500ug)
Lane 3: Hela whole cell lysate (10ug)

anti-GPI antibody Immunoprecipitation (IP) (IP) image
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NCBI/Uniprot data below describe general gene information for GPI. It may not necessarily be applicable to this product.
 NCBI GI #    18201905
 NCBI GeneID    2821
 NCBI Accession #    NP_000166.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_000175.3 [Other Products]
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 UniProt Primary Accession #    P06744 [Other Products]
 UniProt Secondary Accession #    Q9BRD3; Q9BSK5; Q9UHE6; B4DG39 [Other Products]
 UniProt Related Accession #    P06744 [Other Products]
 Molecular Weight    64,325 Da
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 NCBI Official Full Name    glucose-6-phosphate isomerase isoform 2
 NCBI Official Synonym Full Names    glucose-6-phosphate isomerase
 NCBI Official Symbol    GPI [Similar Products]
 NCBI Official Synonym Symbols   
AMF; NLK; PGI; PHI; GNPI; SA36; SA-36
[Similar Products]
 NCBI Protein Information    glucose-6-phosphate isomerase
 UniProt Protein Name    Glucose-6-phosphate isomerase
 UniProt Synonym Protein Names   
Autocrine motility factor; AMF; Neuroleukin; NLK; Phosphoglucose isomerase; PGI; Phosphohexose isomerase; PHI; Sperm antigen 36; SA-36
 Protein Family    Glucose-6-phosphate isomerase
 UniProt Gene Name    GPI [Similar Products]
 UniProt Synonym Gene Names    GPI; AMF; NLK; PGI; PHI; SA-36 [Similar Products]
 UniProt Entry Name    G6PI_HUMAN
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 NCBI Summary for GPI    This gene encodes a member of the glucose phosphate isomerase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In the cytoplasm, the gene product functions as a glycolytic enzyme (glucose-6-phosphate isomerase) that interconverts glucose-6-phophsate and fructose-6-phosphate. Extracellularly, the encoded protein (also referred to as neuroleukin) functions as a neurotrophic factor that promotes survival of skeletal motor neurons and sensory neurons, and as a lymphokine that induces immunoglobulin secretion. The encoded protein is also referred to as autocrine motility factor based on an additional function as a tumor-secreted cytokine and angiogenic factor. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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 UniProt Comments for GPI    G6PI: belongs to the GPI family whose members encode multifunctional phosphoglucose isomerase proteins involved in energy pathways. A dimeric enzyme that catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. Functions in different capacities inside and outside the cell. In the cytoplasm, the gene product is involved in glycolysis and gluconeogenesis, while outside the cell it functions as a neurotrophic factor for spinal and sensory neurons. Defects in this gene are the cause of nonspherocytic hemolytic anemia and a severe enzyme deficiency can be associated with hydrops fetalis, immediate neonatal death and neurological impairment.

Protein type: Carbohydrate Metabolism - glycolysis and gluconeogenesis; Cytokine; Carbohydrate Metabolism - starch and sucrose; Apoptosis; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - pentose phosphate pathway; Isomerase; EC 5.3.1.9

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: cytoplasm; cytosol; extracellular space; membrane; neuron projection; nucleoplasm; plasma membrane

Molecular Function: cytokine activity; glucose-6-phosphate isomerase activity; growth factor activity; intramolecular transferase activity; monosaccharide binding; ubiquitin protein ligase binding

Biological Process: aldehyde catabolic process; angiogenesis; carbohydrate metabolic process; gene expression; gluconeogenesis; glucose 6-phosphate metabolic process; glucose metabolic process; hemostasis; humoral immune response; learning and/or memory; methylglyoxal biosynthetic process; negative regulation of caspase activity; negative regulation of neuron apoptosis; transcription initiation from RNA polymerase II promoter

Disease: Hemolytic Anemia, Nonspherocytic, Due To Glucose Phosphate Isomerase Deficiency
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 Research Articles on GPI    1. The association of ENO1 and GPI with postthaw sperm viability and motility was confirmed using Pearson's linear correlation. ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Pathways associated with anti-GPI antibodyDiseases associated with anti-GPI antibody
 Products by Pathway  Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Amino Sugar And Nucleotide Sugar Metabolism Pathway antibodies  Amino Sugar And Nucleotide Sugar Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Carbon Metabolism Pathway antibodies  Carbon Metabolism Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 Gluconeogenesis Pathway antibodies  Gluconeogenesis Pathway Diagram
 Glucose Metabolism Pathway antibodies  Glucose Metabolism Pathway Diagram
 Glycolysis Pathway antibodies  Glycolysis Pathway Diagram
 Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate antibodies  Glycolysis (Embden-Meyerhof Pathway), Glucose => Pyruvate Diagram
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >1637 publications with GPI and Nervous System Diseases
 Anemia Antibodies  >549 publications with GPI and Anemia
 Inflammation Antibodies  >537 publications with GPI and Inflammation
 Death Antibodies  >536 publications with GPI and Death
 Disease Models, Animal Antibodies  >506 publications with GPI and Disease Models, Animal
 Necrosis Antibodies  >473 publications with GPI and Necrosis
 Anemia, Hemolytic Antibodies  >463 publications with GPI and Anemia, Hemolytic
 Ovarian Neoplasms Antibodies  >348 publications with GPI and Ovarian Neoplasms
 Neoplasms, Experimental Antibodies  >270 publications with GPI and Neoplasms, Experimental
 Heart Diseases Antibodies  >268 publications with GPI and Heart Diseases
Organs/Tissues associated with anti-GPI antibody
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >4057 publications with GPI and Blood
 Brain Antibodies  >2108 publications with GPI and Brain
 Nerve Antibodies  >707 publications with GPI and Nerve
 Bone Antibodies  >655 publications with GPI and Bone
 Muscle Antibodies  >649 publications with GPI and Muscle
 Vascular Antibodies  >548 publications with GPI and Vascular
 Lung Antibodies  >535 publications with GPI and Lung
 Kidney Antibodies  >521 publications with GPI and Kidney
 Liver Antibodies  >502 publications with GPI and Liver
 Ganglia Antibodies  >474 publications with GPI and Ganglia
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