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anti-GAD2 antibody :: Rabbit Glutamate Decarboxylase 65,67 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS618215 anti-GAD2 antibody
Unit / Price
0.1 mL  /  $720 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Glutamate Decarboxylase 65,67 (GAD2), Polyclonal Antibody

★Popular Item★
 Also Known As   

Glutamate Decarboxylase 65,67 (GAD 65,67)

 Product Synonym Names    Anti -Glutamate Decarboxylase 65,67 (GAD 65,67)
 Product Gene Name   

anti-GAD2 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 10; NC_000010.10 (26505236..26593491). Location: 10p11.23
 OMIM    138275
 3D Structure    ModBase 3D Structure for Q05329
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Feline, Human, Mouse
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 Specificity    Recognizes both molecular forms of rat glutamic acid decarboxylase (GAD65 and GAD67), the enzyme which converts glutamic acid to gama-aminobutyric acid (GABA), the major inhibitory transmitter in the higher brain region.
 Purity/Purification    Purified
Purified by ammonium sulfate precipitation.
 Form/Format    Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide.
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 Immunogen    Synthetic peptide corresponding to DFLIEEIERLGQDL from the C-terminus of rat GAD.
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-GAD2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-GAD2 antibody

   Glutamic acid decarboxylase (GAD; E.C. 4.1.1.15) is the enzyme responsible for the conversion of glutamic acid to gamma-aminobutyric acid (GABA), the major inhibitory transmitter in higher brain regions, and putative paracrine hormone in pancreatic islets. Two molecular forms of GAD (65kD and 67kD, 64% aa identity between forms) are highly conserved and both forms are expressed in the CNS, pancreatic islet cells, testis, oviduct and ovary. The isoforms are regionally distributed cytoplasmically in the brains of rats and mice (Sheikh, S. et al. 1999). GAD65 is an ampiphilic, membrane-anchored protein (585aa) encoded on human chromosome10. GAD67 is cytoplasmic (594aa) and is encoded on chromosome 2. GAD expression changes during neural development in rat spinal cord. GAD65 is expressed transiently in commissural axons around E13 but is down regulated the next day while GAD67 expression increases mostly in the somata of those neurons (Phelps, P. et al. 1999). In mature rat pancreas, GAD65 and GAD67 appear to be differentially localized, GAD65 primarily in insulin-containing beta cells and GAD67 in glucagon-containing (A) cells (Li, L. et al. 1995). Changes in GAD65/GAD67 distributions have also been correlated with certain disease states such as IDDM and SMS.
 Product Categories/Family for anti-GAD2 antibody    Antibodies; Abs to Enzymes, Decarboxylase
 Applications Tested/Suitable for anti-GAD2 antibody   

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

 Application Notes for anti-GAD2 antibody    Suitable for use in Immunohistochemistry, ELISA, Western Blot and Immunofluorescence.
Dilution: Immunohistochemistry (Frozen): 1:200-1:5000
Immunohistochemistry: Paraffin sections
Western Blot: 1:1000-1:10,000. A doublet at ~65/67kD is expected.
Optimal working dilutions to be determined by researcher.

Recommended Positive Control Tissues: Brain
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NCBI/Uniprot data below describe general gene information for GAD2. It may not necessarily be applicable to this product.
 NCBI GI #    4503875
 NCBI GeneID    2572
 NCBI Accession #    NP_000809.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000818.2 [Other Products]
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 UniProt Primary Accession #    Q05329 [Other Products]
 UniProt Secondary Accession #    Q9UD87 [Other Products]
 UniProt Related Accession #    Q05329 [Other Products]
 Molecular Weight    65,411 Da [Similar Products]
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 NCBI Official Full Name    glutamate decarboxylase 2
 NCBI Official Synonym Full Names    glutamate decarboxylase 2 (pancreatic islets and brain, 65kDa)
 NCBI Official Symbol    GAD2 [Similar Products]
 NCBI Official Synonym Symbols   
GAD65
[Similar Products]
 NCBI Protein Information    glutamate decarboxylase 2; GAD-65; 65 kDa glutamic acid decarboxylase; Glutamate decarboxylase-2 (pancreas); glutamate decarboxylase 65 kDa isoform
 UniProt Protein Name    Glutamate decarboxylase 2
 UniProt Synonym Protein Names   
65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
 Protein Family    Glutamate decarboxylase
 UniProt Gene Name    GAD2 [Similar Products]
 UniProt Synonym Gene Names    GAD65; GAD-65 [Similar Products]
 UniProt Entry Name    DCE2_HUMAN
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 NCBI Summary for GAD2    This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008]
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 UniProt Comments for GAD2    GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.

Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - beta-alanine; Lyase; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Other Amino Acids Metabolism - taurine and hypotaurine

Chromosomal Location of Human Ortholog: 10p11.23

Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cell junction; cytosol

Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding

Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process
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 Research Articles on GAD2    1. The study demonstrated a statistically significant difference in the frequency of the GAD2 (+83987 T/A) genes between obese patients and controls in Taiwanese subjects.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-GAD2 antibodyPathways associated with anti-GAD2 antibody
 Reference Product  PubMed Publications
 GAD1 antibody  >45 publications with GAD2 and GAD1
 GLUL antibody  >1 publications with GAD2 and GLUL
 SLC32A1 antibody  >1 publications with GAD2 and SLC32A1
 INS antibody  >1 publications with GAD2 and INS
 ABAT antibody  >1 publications with GAD2 and ABAT
 Products by Pathway  Pathway Diagram
 Alanine And Aspartate Metabolism Pathway antibodies  Alanine And Aspartate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Alanine, Aspartate And Glutamate Metabolism Pathway antibodies  Alanine, Aspartate And Glutamate Metabolism Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 Butanoate Metabolism Pathway antibodies  Butanoate Metabolism Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA (gamma-Aminobutyrate) Shunt Pathway antibodies  GABA (gamma-Aminobutyrate) Shunt Pathway Diagram
 GABA Synthesis Pathway antibodies  GABA Synthesis Pathway Diagram
 GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies  GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Diseases associated with anti-GAD2 antibodyOrgans/Tissues associated with anti-GAD2 antibody
 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >17 publications with GAD2 and Mental Disorders
 Nervous System Diseases Antibodies  >11 publications with GAD2 and Nervous System Diseases
 Brain Diseases Antibodies  >9 publications with GAD2 and Brain Diseases
 Schizophrenia Antibodies  >8 publications with GAD2 and Schizophrenia
 Epilepsy Antibodies  >6 publications with GAD2 and Epilepsy
 Seizures Antibodies  >6 publications with GAD2 and Seizures
 Mood Disorders Antibodies  >4 publications with GAD2 and Mood Disorders
 Inflammation Antibodies  >4 publications with GAD2 and Inflammation
 Disease Models, Animal Antibodies  >3 publications with GAD2 and Disease Models, Animal
 Depressive Disorder Antibodies  >3 publications with GAD2 and Depressive Disorder
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >27 publications with GAD2 and Brain
 Pancreas Antibodies  >3 publications with GAD2 and Pancreas
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