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anti-HRASLS2 antibody :: Goat HRASLS2 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS422003
Immunohistochemistry (IHC)
Unit / Price
0.1 mg  /  $300 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

HRASLS2, Polyclonal Antibody

 Also Known As   

Goat anti-HRASLS2 Antibody

 Product Synonym Names    HRASLS2; HRAS-like suppressor 2; FLJ20556; HRASLS2 antibody; HRAS-like suppressor 2 antibody; FLJ20556 antibody
 Product Gene Name   

anti-HRASLS2 antibody

[Similar Products]
 Antibody/Peptide Pairs    HRASLS2 peptide (MBS425297) is used for blocking the activity of HRASLS2 antibody (MBS422003)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    QELPYSLTSD NC
 OMIM    613866
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 Clonality    Polyclonal
 Host    Goat
 Species Reactivity    Tested: Human; Expected from sequence similarity: Human
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 Purity/Purification    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
 Form/Format    Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
 Concentration    100ug specific antibody in 200ul (lot specific)
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 Immunogen    Peptide with sequence QELPYSLTSDNC, from the internal region of the protein sequence according to NP_060348.1.
 Epitope    Internal region
 Note    This design was based on provisional sequence data.
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 Preparation and Storage    Aliquot and store at -20 degree C. Minimize freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2015 Certified Laboratory.
 Other Notes    Small volumes of anti-HRASLS2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Applications Tested/Suitable for anti-HRASLS2 antibody   

Peptide ELISA (EIA), Immunohistochemistry (IHC)

 Application Notes for anti-HRASLS2 antibody    Peptide ELISA: Antibody detection limit dilution 1: 16000.
Immunohistochemistry: In paraffin embedded Human Liver shows a granular pattern in the cytoplasm of hepatocytes. Recommended concentration, 5-10ug/ml.
Western Blot: Preliminary experiments gave an approx.21kDa band in Human Kidney lysates after 0.5 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 17.4kDa according to NP_060348.1 (provisional). The 21kDa band was successfully blocked by incubation with the immunizing peptide.
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 Immunohistochemistry (IHC) of anti-HRASLS2 antibody    (5ug/ml) staining of paraffin embedded Human Liver. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
anti-HRASLS2 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for HRASLS2. It may not necessarily be applicable to this product.
 NCBI GI #    8923526
 NCBI GeneID    54979
 NCBI Accession #    NP_060348.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_017878.1 [Other Products]
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 UniProt Secondary Accession #    B9A7L8 [Other Products]
 UniProt Related Accession #    Q9NWW9 [Other Products]
 Molecular Weight    17,394 Da
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 NCBI Official Full Name    HRAS-like suppressor 2
 NCBI Official Synonym Full Names    HRAS like suppressor 2
 NCBI Official Symbol    HRASLS2 [Similar Products]
 NCBI Official Synonym Symbols   
PLA1/2-2
[Similar Products]
 NCBI Protein Information    HRAS-like suppressor 2
 UniProt Protein Name    HRAS-like suppressor 2
 Protein Family    HRAS-like suppressor
 UniProt Gene Name    HRASLS2 [Similar Products]
 UniProt Entry Name    HRSL2_HUMAN
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 UniProt Comments for HRASLS2    HRASLS2: Exhibits PLA1/2 activity, catalyzing the calcium- independent hydrolysis of acyl groups in various phosphotidylcholines (PC) and phosphatidylethanolamine (PE). For most substrates, PLA1 activity is much higher than PLA2 activity. Catalyzes N-acylation of PE using both sn-1 and sn-2 palmitoyl groups of PC as acyl donor. Also catalyzes O-acylation converting lyso-PC into PC. Belongs to the H-rev107 family.

Protein type: EC 2.3.1.-; EC 3.1.1.-

Chromosomal Location of Human Ortholog: 11q12.3

Cellular Component: cytoplasm; integral to membrane

Molecular Function: hydrolase activity; transferase activity, transferring acyl groups

Biological Process: lipid catabolic process
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 Research Articles on HRASLS2    1. Data show that acyl-modified forms of HRAS-like tumor suppressors HRASLS2 and HRASLS3 mimicking lipolytic activity of lecithin retinol acyltransferase LRAT.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-HRASLS2 antibody
 Organ/Tissue Name  Pubmed Publications
 Kidney Antibodies  >1 publications with HRASLS2 and Kidney
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