Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow HNF4A rightarrow Polyclonal HNF4A  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • Heat Shock Protein 27 (HSP27) ELISA Kit
 • beta2 Microglobulin (beta2-MG) ELISA Kit
 • Homer1 Antibody
 • Fibrinogen Degradation Product (FDP) ELISA Kit
 • GRIA2 Antibody
 • ABL1 Blocking Peptide
 • Muscle, skeletal, receptor tyrosine kinase (MuSK) ELISA Kit
 • 7-Aminoactinomycin D (7-AAD) Antibody
 • Vascular Endothelial Growth Factor (VEGF) ELISA Kit
 • glutathione S-transferase mu 5 (GSTM5) Antibody
 • DDX11 Antibody
 • cortisol (COR) ELISA Kit
 • IL-13 (IL13) ELISA Kit
 • Morphine Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-HNF4A antibody :: Rabbit Hepatocyte nuclear factor 4-alpha Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS177518
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hepatocyte nuclear factor 4-alpha (HNF4A), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-HNF-4-alpha Antibody

 Product Synonym Names    FLJ39654 antibody; Hepatic nuclear factor 4 alpha antibody; Hepatocyte nuclear factor 4 alpha antibody; Hepatocyte nuclear factor 4 antibody; Hepatocyte nuclear factor 4-alpha antibody; HNF 4 alpha antibody; HNF 4 antibody; HNF-4-alpha antibody; HNF4 antibody; HNF4 antibody; HNF4A antibody; HNF4A_HUMAN antibody; HNF4a7 antibody; HNF4a8 antibody; HNF4a9 antibody; Hnf4alpha antibody; HNF4alpha10/11/12 antibody; MODY 1 antibody; MODY antibody; MODY1 antibody; NR2A1 antibody; NR2A1 antibody; NR2A21 antibody; Nuclear receptor subfamily 2 group A member 1 antibody; OTTHUMP00000031060 antibody; OTTHUMP00000031062 antibody; TCF 14 antibody; TCF antibody; TCF-14 antibody; TCF14 antibody; TCF14 antibody; Tcf4 antibody; Transcription factor 14, hepatic nuclear factor antibody; Transcription factor 14 antibody; Transcription factor HNF 4 antibody; Transcription factor HNF-4 antibody; Transcription factor HNF4 antibody; hepatocyte nuclear factor 4, alpha
 Product Gene Name   

anti-HNF4A antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 OMIM    gene 606391
 3D Structure    ModBase 3D Structure for P41235
Section Bar
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat. No cross reactivity with other proteins.
Section Bar
 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Section Bar
 Reconstitution    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
 Immunogen    E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively.
 Relevant Detection Systems    We provides a series of assays reacted with primary antibodies. Antibody can be supported by chemiluminescence kit (MBS176460) in WB, supported by MBS176451 in IHC(P) and IHC(F).
Section Bar
 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-HNF4A antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Product Description specifically for anti-HNF4A antibody

   Background: Hepatocyte nuclear factor 4 alpha (HNF4A), also known as NR2A1, is a nuclear receptor that in humans is encoded by the HNF4A gene. It is mapped to 20q13.12. HNF4A is a nuclear transcription factor that binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene plays a role in development of the liver, kidney, and intestines. HNF4A is required for the PXR and CAR-mediated transcriptional activation of CYP3A4. This gene also plays a pivotal role in the expression and synthesis of SHBG, an important glycoprotein made primarily in the liver, which in addition to lowering insulin-resistance also serves in reducing levels of free Oestrogen as-well as prolonging the half-life of Testosterone.
 Applications Tested/Suitable for anti-HNF4A antibody   

Western Blot (WB), Immunohistochemistry (IHC-P)

 Application Notes for anti-HNF4A antibody    Western Blot: Concentration: 0.1-0.5ug/ml; Tested Species: Hu, Rat
Immunohistochemistry (Paraffin-embedded Section): Concentration: 0.5-1ug/ml; Tested Species: Hu, Ms, Rat; Antigen Retrieval: By Heat
Immunohistochemistry (Frozen Section): Concentration: 0.5-1ug/ml; Tested Species: Ms, Rat.
WB: The detection limit for HNF-4-alpha is approximately 0.25ng/lane under reducing conditions.
Tested Species: In-house tested species with positive results.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Section Bar
 Western Blot (WB) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, Western blotting
All lanes: Anti HNF-4-alpha at 0.5ug/ml
WB: Recombinant Human HNF-4-alpha Protein 0.5ng
Predicted bind size: 38KD
Observed bind size: 38KD
anti-HNF4A antibody Western Blot (WB) (WB) image
Section Bar
 Western Blot (WB) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, Western blotting
All lanes: Anti HNF-4-alpha at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Lane 3: RH35 Whole Cell Lysate at 40ug
Lane 4: HELA Whole Cell Lysate at 40ug
Lane 5: HEPG2 Whole Cell Lysate at 40ug
Predicted bind size: 53KD
Observed bind size: 53KD
anti-HNF4A antibody Western Blot (WB) (WB) image
Section Bar
 Immunohistochemistry (IHC) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, IHC(P)
IHC(P): Rat Kidney Tissue
anti-HNF4A antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Immunohistochemistry (IHC) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, IHC(P)
IHC(P): Mouse Kidney Tissue
anti-HNF4A antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Immunohistochemistry (IHC) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
anti-HNF4A antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Immunohistochemistry (IHC) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, IHC(F)
IHC(F): Mouse Liver Tissue
anti-HNF4A antibody Immunohistochemistry (IHC) (IHC) image
Section Bar
 Immunohistochemistry (IHC) of anti-HNF4A antibody    Anti- HNF-4-alpha antibody, IHC(F)
IHC(F): Rat Kindey Tissue
anti-HNF4A antibody Immunohistochemistry (IHC) (IHC) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for HNF4A. It may not necessarily be applicable to this product.
 NCBI GI #    31077205
 NCBI GeneID    3172
 NCBI Accession #    NP_000448.3 [Other Products]
 NCBI GenBank Nucleotide #    NM_000457.4 [Other Products]
Section Bar
 UniProt Primary Accession #    P41235 [Other Products]
 UniProt Secondary Accession #    O00659; O00723; Q14540; Q5QPB8; Q6B4V5; Q6B4V6; Q6B4V7; Q92653; Q92654; A5JW41; B2RPP8 [Other Products]
 UniProt Related Accession #    P41235 [Other Products]
 Molecular Weight    43,988 Da
Table BarTOPTable Bar
 NCBI Official Full Name    hepatocyte nuclear factor 4-alpha isoform HNF4alpha2
 NCBI Official Synonym Full Names    hepatocyte nuclear factor 4, alpha
 NCBI Official Symbol    HNF4A [Similar Products]
 NCBI Official Synonym Symbols   
TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
[Similar Products]
 NCBI Protein Information    hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1
 UniProt Protein Name    Hepatocyte nuclear factor 4-alpha
 UniProt Synonym Protein Names   
Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; TCF-14; Transcription factor HNF-4
 Protein Family    Hepatocyte nuclear factor
 UniProt Gene Name    HNF4A [Similar Products]
 UniProt Synonym Gene Names    HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14 [Similar Products]
 UniProt Entry Name    HNF4A_HUMAN
Section Bar
 NCBI Summary for HNF4A    The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]
Section Bar
 UniProt Comments for HNF4A    HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.

Protein type: Transcription factor; DNA-binding; Nuclear receptor

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; ligand-dependent nuclear receptor activity; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; transcription factor activity; fatty acid binding; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; regulation of lipid metabolic process; intracellular receptor-mediated signaling pathway; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; xenobiotic metabolic process; regulation of gastrulation; response to glucose stimulus; ornithine metabolic process; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis

Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent
Table BarTOPTable Bar
 Research Articles on HNF4A    1. HNF4alpha-NF-kappaB feedback circuit including miR-124, miR-7, and miR-21 identified in hepatocellular carcinoma
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Pathways associated with anti-HNF4A antibodyDiseases associated with anti-HNF4A antibody
 Products by Pathway  Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 AMPK Signaling Pathway antibodies  AMPK Signaling Pathway Diagram
 Developmental Biology Pathway antibodies  Developmental Biology Pathway Diagram
 FOXA2 And FOXA3 Transcription Factor Networks Pathway antibodies  FOXA2 And FOXA3 Transcription Factor Networks Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Generic Transcription Pathway antibodies  Generic Transcription Pathway Diagram
 HIF-1-alpha Transcription Factor Network Pathway antibodies  HIF-1-alpha Transcription Factor Network Pathway Diagram
 Integrated Pancreatic Cancer Pathway antibodies  Integrated Pancreatic Cancer Pathway Diagram
 Maturity Onset Diabetes Of The Young Pathway antibodies  Maturity Onset Diabetes Of The Young Pathway Diagram
 Disease Name  Pubmed Publications
 Diabetes Mellitus, Type 2 Antibodies  >200 publications with HNF4A and Diabetes Mellitus, Type 2
 Liver Diseases Antibodies  >92 publications with HNF4A and Liver Diseases
 Liver Neoplasms Antibodies  >50 publications with HNF4A and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >49 publications with HNF4A and Carcinoma, Hepatocellular
 Fatty Liver Antibodies  >39 publications with HNF4A and Fatty Liver
 Hyperglycemia Antibodies  >35 publications with HNF4A and Hyperglycemia
 Insulin Resistance Antibodies  >25 publications with HNF4A and Insulin Resistance
 Inflammation Antibodies  >24 publications with HNF4A and Inflammation
 Cardiovascular Diseases Antibodies  >17 publications with HNF4A and Cardiovascular Diseases
 Hepatitis Antibodies  >17 publications with HNF4A and Hepatitis
Organs/Tissues associated with anti-HNF4A antibody
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >337 publications with HNF4A and Liver
 Kidney Antibodies  >44 publications with HNF4A and Kidney
 Intestine Antibodies  >40 publications with HNF4A and Intestine
 Stomach Antibodies  >11 publications with HNF4A and Stomach
 Lung Antibodies  >6 publications with HNF4A and Lung
 Placenta Antibodies  >4 publications with HNF4A and Placenta
 Mammary Gland Antibodies  >1 publications with HNF4A and Mammary Gland
 Uncharacterized Tissue Antibodies  >1 publications with HNF4A and Uncharacterized Tissue
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions