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anti-HMG1 antibody :: Rabbit anti-Mouse High Mobility Group Protein 1 (HMG1) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS2007018 (SPECIAL PROMOTION: Get FREE $5 Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment)
Unit / Price
0.01 mg  /  $105 +1 FREE 8GB USB
0.02 mg  /  $125 +1 FREE 8GB USB
0.05 mg  /  $170 +1 FREE 8GB USB
0.1 mg  /  $210 +1 FREE 8GB USB
0.2 mg  /  $315 +1 FREE 8GB USB
1 mg  /  $745 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

High Mobility Group Protein 1 (HMG1), Polyclonal Antibody

★Popular Item★
 Also Known As   

Polyclonal Antibody to High Mobility Group Protein 1 (HMG1)

 Product Gene Name   

anti-HMG1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence    The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF-MGKGDP KKPR GKMSSYAFFV QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR YEREMKTYIP PKGETKKKFK DPNAPKRPPS AFFLFCSEYR PKIKGEHPGL SIGDVAKKLG EMWNNTAADD KQPYEKKAAK LKEKYEKDIA AYRAKGKPDA AKKGVVKAEK SKKKKEEEDD EEDEEDEEEE EEEEDEDEEE DDDDE
 3D Structure    ModBase 3D Structure for P63158
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse
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 Specificity    The antibody is a rabbit polyclonal antibody raised against HMG1. It has been selected for its ability to recognize HMG1 in immunohistochemical staining andwestern blotting.
 Purity/Purification    Affinity Chromatography
 Form/Format    Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
 Concentration    200ug/ml (lot specific)
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 Immunogen    Recombinant HMG1 (Met1~Glu215) expressed in E.coli.
 Conjugated Antibody    The APC conjugated antibody version of this item is also available as catalog #MBS2041950
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 Preparation and Storage    Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
 Other Notes    Small volumes of anti-HMG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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 Product Categories/Family for anti-HMG1 antibody    Knock-Out Validation Antibody
 Applications Tested/Suitable for anti-HMG1 antibody   

Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)

 Application Notes for anti-HMG1 antibody    Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
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 Western Blot (WB) Knockout Verification of anti-HMG1 antibody    Knockout Verification: Lane 1: Wild-type HepG2 cell lysate
Lane 2: HMG1 knockout HepG2 cell lysate
Predicted MW: 25kDa ;Observed MW: 30kDa;Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-HMG1 antibody Western Blot (WB) Knockout Verification (WB) image
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 Western Blot (WB) of anti-HMG1 antibody    Western Blot; Sample: Mouse Kidney lysate; ;Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-HMG1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-HMG1 antibody    Western Blot; Sample: Mouse Cerebrum lysate; ;Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-HMG1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-HMG1 antibody    Western Blot; Sample: Human HepG2 cell lysate; ;Primary Ab: 1ug/ml Rabbit Anti-Mouse HMG1 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;
anti-HMG1 antibody Western Blot (WB) (WB) image
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 Western Blot (WB) of anti-HMG1 antibody    Western Blot; Sample: Recombinant protein.
anti-HMG1 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-HMG1 antibody    DAB staining on fromalin fixed paraffin-embedded Kidney tissue)
anti-HMG1 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for HMG1. It may not necessarily be applicable to this product.
 NCBI GI #    926657659
 NCBI GeneID    15289
 NCBI Accession #    NP_001300823.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001313894.1 [Other Products]
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 UniProt Primary Accession #    P63158 [Other Products]
 UniProt Secondary Accession #    P07155; P27109; P27428 [Other Products]
 UniProt Related Accession #    P63158 [Other Products]
 Molecular Weight    24,894 Da
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 NCBI Official Full Name    high mobility group protein B1
 NCBI Official Synonym Full Names    high mobility group box 1
 NCBI Official Symbol    Hmgb1 [Similar Products]
 NCBI Official Synonym Symbols   
p30; Hmg1; HMG-1; SBP-1
[Similar Products]
 NCBI Protein Information    high mobility group protein B1
 UniProt Protein Name    High mobility group protein B1
 UniProt Synonym Protein Names   
High mobility group protein 1; HMG-1
 Protein Family    High mobility group protein
 UniProt Gene Name    Hmgb1 [Similar Products]
 UniProt Synonym Gene Names    Hmg-1; Hmg1; HMG-1 [Similar Products]
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 NCBI Summary for HMG1    This gene encodes a protein that belongs to the High Mobility Group-box superfamily. The encoded non-histone, nuclear DNA-binding protein regulates transcription, and is involved in organization of DNA. This protein plays a role in several cellular processes, including inflammation, cell differentiation and tumor cell migration. Multiple pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Sep 2015]
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 UniProt Comments for HMG1    HMGB1: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells. Component of the RAG complex composed of core components RAG1 and RAG2, and associated component HMGB1 or HMGB2. Belongs to the HMGB family.

Protein type: DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 5 G3|5 89.18 cM

Cellular Component: cytoplasm; early endosome; extracellular region; extracellular space; neuron projection; nucleus

Molecular Function: bent DNA binding; calcium-dependent protein kinase regulator activity; crossed form four-way junction DNA binding; cytokine activity; double-stranded DNA binding; double-stranded RNA binding; open form four-way junction DNA binding; phosphatidylserine binding; protein binding; protein kinase activator activity; single-stranded RNA binding

Biological Process: base-excision repair; chromatin assembly; DNA geometric change; endothelial cell proliferation; eye development; induction of positive chemotaxis; inflammatory response; lung development; macrophage activation involved in immune response; myeloid dendritic cell activation; negative regulation of apoptotic cell clearance; plasmacytoid dendritic cell activation; positive regulation of cell migration; positive regulation of ERK1 and ERK2 cascade; positive regulation of glycogen catabolic process; positive regulation of innate immune response; positive regulation of interferon-alpha production; positive regulation of interferon-beta production; positive regulation of interleukin-1 beta secretion; positive regulation of interleukin-6 production; positive regulation of mesenchymal cell proliferation; positive regulation of mitotic cell cycle; positive regulation of myeloid cell differentiation; positive regulation of NIK/NF-kappaB signaling; positive regulation of protein phosphorylation; positive regulation of toll-like receptor 2 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of wound healing; regulation of autophagy; regulation of nucleotide-excision repair; regulation of T cell mediated immune response to tumor cell; regulation of tolerance induction; response to glucocorticoid stimulus
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 Research Articles on HMG1    1. These results suggest that HMGB1-modulated TLR5 signaling is responsible for pain hypersensitivity.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-HMG1 antibodyPathways associated with anti-HMG1 antibody
 Reference Product  PubMed Publications
 Tlr4 antibody  >547 publications with HMG1 and Tlr4
 Hmgb2 antibody  >260 publications with HMG1 and Hmgb2
 Ager antibody  >44 publications with HMG1 and Ager
 Rela antibody  >39 publications with HMG1 and Rela
 S100b antibody  >30 publications with HMG1 and S100b
 Ikbkb antibody  >17 publications with HMG1 and Ikbkb
 Trp53 antibody  >16 publications with HMG1 and Trp53
 Chuk antibody  >5 publications with HMG1 and Chuk
 Nfkb1 antibody  >5 publications with HMG1 and Nfkb1
 Nfkb2 antibody  >1 publications with HMG1 and Nfkb2
 Products by Pathway  Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of DNA Fragmentation Factor Pathway antibodies  Activation Of DNA Fragmentation Factor Pathway Diagram
 Advanced Glycosylation Endproduct Receptor Signaling Pathway antibodies  Advanced Glycosylation Endproduct Receptor Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Induced DNA Fragmentation Pathway antibodies  Apoptosis Induced DNA Fragmentation Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Base Excision Repair Pathway antibodies  Base Excision Repair Pathway Diagram
 Cytosolic Sensors Of Pathogen-associated DNA Pathway antibodies  Cytosolic Sensors Of Pathogen-associated DNA Pathway Diagram
 DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway antibodies  DEx/H-box Helicases Activate Type I IFN And Inflammatory Cytokines Production Pathway Diagram
Diseases associated with anti-HMG1 antibodyOrgans/Tissues associated with anti-HMG1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >1884 publications with HMG1 and Inflammation
 Necrosis Antibodies  >975 publications with HMG1 and Necrosis
 Sepsis Antibodies  >610 publications with HMG1 and Sepsis
 Disease Models, Animal Antibodies  >564 publications with HMG1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >351 publications with HMG1 and Nervous System Diseases
 Liver Diseases Antibodies  >264 publications with HMG1 and Liver Diseases
 Heart Diseases Antibodies  >186 publications with HMG1 and Heart Diseases
 Myocardial Ischemia Antibodies  >145 publications with HMG1 and Myocardial Ischemia
 Kidney Diseases Antibodies  >127 publications with HMG1 and Kidney Diseases
 Fibrosis Antibodies  >124 publications with HMG1 and Fibrosis
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >550 publications with HMG1 and Liver
 Lung Antibodies  >526 publications with HMG1 and Lung
 Brain Antibodies  >432 publications with HMG1 and Brain
 Heart Antibodies  >249 publications with HMG1 and Heart
 Muscle Antibodies  >244 publications with HMG1 and Muscle
 Kidney Antibodies  >223 publications with HMG1 and Kidney
 Bone Antibodies  >182 publications with HMG1 and Bone
 Intestine Antibodies  >127 publications with HMG1 and Intestine
 Thymus Antibodies  >106 publications with HMG1 and Thymus
 Bone Marrow Antibodies  >99 publications with HMG1 and Bone Marrow
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