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anti-H3 antibody :: Rabbit Histone H3, trimethyl Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS613333 anti-H3 antibody
Unit / Price
0.1 mg  /  $765 +1 FREE 8GB USB
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Histone H3, trimethyl, Polyclonal Antibody

 Also Known As   

Histone H3, trimethyl (Lys9)

 Product Synonym Names    Anti -Histone H3, trimethyl (Lys9)
 Product Gene Name   

anti-H3 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    C elegans, Human
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 Specificity    Recognizes human trimethyl-histone H3 (Lys9), Mr 17kD. Species Crossreactivity
 Purity/Purification    Affinity Purified
Purified by Protein A affinity chromatography.
 Form/Format    Supplied as a liquid in 0.1M Tris-glycine, pH7.4, 0.15M sodium chloride, 0.05% sodium azide, 30% glycerol.
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 Immunogen    A synthetic peptide containing the 2X-branched sequence …AR[me3K]ST… in which me3K corresponds to trimethyl-lysine at residue 9 of human histone H3 (KLH).
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-H3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-H3 antibody

   Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the "beads on a string" structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
 Product Categories/Family for anti-H3 antibody    Antibodies; Abs to Histones
 Applications Tested/Suitable for anti-H3 antibody   

Western Blot (WB), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA)

 Application Notes for anti-H3 antibody    Suitable for use in Western Blot, Dot Blot, Peptide Inhibition Assay and Immunofluorescence.
Dilution: Western Blot: 1:500 of this lot detected trimethyl Histone H3 in acid extracted proteins from HeLa cells.
Peptide Inhibition: Specificity of this lot confirmed by the ability of 10uM of the immunizing peptide to completely abolish detection of histone H3 in Western Blot of HeLa acid extracts. No inhibition of detection was observed by preabsorption of the antibody with 10uM histone H3 peptide containing mono- or dimethyl-lysine 9, or mono-, di- or trimethyl-lysine 27 modifications.
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NCBI/Uniprot data below describe general gene information for H3. It may not necessarily be applicable to this product.
 NCBI GI #    47551065
 NCBI GeneID    373335
 NCBI Accession #    NP_999709.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_214544.1 [Other Products]
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 NCBI Official Full Name    histone H3
 NCBI Official Symbol    H3 [Similar Products]
 NCBI Protein Information    histone H3
 Protein Family    Tyrosine phosphatase
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 Research Articles on H3    1. PHD domain of the only known invertebrate homolog of RAG2, the SpRAG2L protein of the purple sea urchin (Strongylocentrotus purpuratus) also binds to methylated histones, but with a unique preference for H3K4me2
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Organs/Tissues associated with anti-H3 antibody
 Organ/Tissue Name  Pubmed Publications
 Bone Antibodies  >13 publications with H3 and Bone
 Connective Tissue Antibodies  >4 publications with H3 and Connective Tissue
 Heart Antibodies  >3 publications with H3 and Heart
 Muscle Antibodies  >3 publications with H3 and Muscle
 Brain Antibodies  >3 publications with H3 and Brain
 Vascular Antibodies  >2 publications with H3 and Vascular
 Embryonic Tissue Antibodies  >2 publications with H3 and Embryonic Tissue
 Ear Antibodies  >1 publications with H3 and Ear
 Kidney Antibodies  >1 publications with H3 and Kidney
 Umbilical Cord Antibodies  >1 publications with H3 and Umbilical Cord
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