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anti-HRK antibody :: Rabbit anti-Human, Mouse Hrk Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS151175
Western Blot (WB)
Unit / Price
0.02 mg  /  $140 +1 FREE 8GB USB
0.1 mg  /  $355 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

Hrk, Polyclonal Antibody

 Also Known As   

Hrk Antibody

 Product Synonym Names    Hrk; DP5; HARAKIRI; BID3; Activator of apoptosis harakiri; BH3-interacting domain-containing protein 3; harakiri, BCL2 interacting protein (contains only BH3 domain)
 Product Gene Name   

anti-HRK antibody

[Similar Products]
 Antibody/Peptide Pairs    Hrk peptide (MBS151950) is used for blocking the activity of Hrk antibody (MBS151175)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    603447
 3D Structure    ModBase 3D Structure for O00198
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Purity/Purification    Hrk Antibody is affinity chromatography purified via peptide column.
 Form/Format    Liquid
 Concentration    1 mg/mL (lot specific)
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 Conjugate    Unconjugated
 Immunogen    HRK antibody was raised against a 15 amino acid synthetic peptide from near the center of human Hrk.
 Buffer    Hrk Antibody is supplied in PBS containing 0.02% sodium azide.
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 Preparation and Storage    Hrk antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
 Other Notes    Small volumes of anti-HRK antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-HRK antibody

   Hrk Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Hrk, a pro-apoptotic member of the Bcl-2 homology domain-3 (BH3)-only group of the Bcl-2 family of proteins, was also identified as novel protein induced during programmed neuronal death. It lacks significant homology to other Bcl-2 family members except for an 8-amino acid region that is similar to the BH3 motif of Bik. Hrk regulates apoptosis through interaction with the anti-apoptotic proteins Bcl-2 and Bcl-XL via this domain. It does not interact with the pro-apoptotic proteins Bax, Bak, or Bcl-XS. Hrk localizes to mitochondrial membranes in a pattern similar to that previously reported for Bcl-2 and Bcl-XL.
 Applications Tested/Suitable for anti-HRK antibody   

ELISA (EIA), Western Blot (WB)

 Application Notes for anti-HRK antibody    Hrk antibody can be used for the detection of Hrk by Western blot at 2.5 - 5 mug/mL. Despite its predicted molecular weight, Hrk often migrates at 12-15 kDa.
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 Western Blot (WB) of anti-HRK antibody    Western blot analysis of Hrk in mouse pancreas tissue lysate with Hrk antibody at (A) 2.5 and (B) 5 μg/mL.
anti-HRK antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for HRK. It may not necessarily be applicable to this product.
 NCBI GI #    4504493
 NCBI GeneID    8739
 NCBI Accession #    NP_003797 [Other Products]
 NCBI GenBank Nucleotide #    NM_003806.2 [Other Products]
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 UniProt Primary Accession #    O00198 [Other Products]
 UniProt Related Accession #    O00198 [Other Products]
 Molecular Weight    9,884 Da
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 NCBI Official Full Name    activator of apoptosis harakiri
 NCBI Official Synonym Full Names    harakiri, BCL2 interacting protein
 NCBI Official Symbol    HRK [Similar Products]
 NCBI Official Synonym Symbols   
DP5; HARAKIRI
[Similar Products]
 NCBI Protein Information    activator of apoptosis harakiri; death protein 5; BCL2-interacting protein; activator of apoptosis Hrk; neuronal death protein DP5; BH3-interacting domain-containing protein 3; harakiri, BCL2 interacting protein (contains only BH3 domain)
 UniProt Protein Name    Activator of apoptosis harakiri
 UniProt Synonym Protein Names   
BH3-interacting domain-containing protein 3; Neuronal death protein DP5
 Protein Family    Activator of apoptosis harakiri
 UniProt Gene Name    HRK [Similar Products]
 UniProt Synonym Gene Names    BID3 [Similar Products]
 UniProt Entry Name    HRK_HUMAN
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 NCBI Summary for HRK    This gene encodes a member of the BCL-2 protein family. Members of this family are involved in activating or inhibiting apoptosis. The encoded protein localizes to intracellular membranes. This protein promotes apoptosis by interacting with the apoptotic inhibitors BCL-2 and BCL-X(L) via its BH3 domain. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012]
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 UniProt Comments for HRK    HRK: Promotes apoptosis.

Protein type: Membrane protein, integral; Apoptosis

Chromosomal Location of Human Ortholog: 12q24.22

Cellular Component: mitochondrion; integral to membrane

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; positive regulation of protein homooligomerization
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 Research Articles on HRK    1. our findings suggest that induction of the BH3-only protein Hrk is a critical step in 2-ME activation of the JNK-induced apoptotic pathway, targeting mitochondria by liberating proapoptotic protein Bak.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-HRK antibodyPathways associated with anti-HRK antibody
 Reference Product  PubMed Publications
 JUN antibody  >32 publications with HRK and JUN
 BAD antibody  >16 publications with HRK and BAD
 BCL2L1 antibody  >14 publications with HRK and BCL2L1
 BIK antibody  >10 publications with HRK and BIK
 BMF antibody  >8 publications with HRK and BMF
 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptosis Modulation And Signaling Pathway antibodies  Apoptosis Modulation And Signaling Pathway Diagram
Diseases associated with anti-HRK antibodyOrgans/Tissues associated with anti-HRK antibody
 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >20 publications with HRK and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with HRK and Brain Diseases
 Inflammation Antibodies  >6 publications with HRK and Inflammation
 Cardiovascular Diseases Antibodies  >5 publications with HRK and Cardiovascular Diseases
 Breast Neoplasms Antibodies  >5 publications with HRK and Breast Neoplasms
 Necrosis Antibodies  >5 publications with HRK and Necrosis
 Disease Models, Animal Antibodies  >3 publications with HRK and Disease Models, Animal
 Mitochondrial Diseases Antibodies  >3 publications with HRK and Mitochondrial Diseases
 Seizures Antibodies  >2 publications with HRK and Seizures
 Hepatitis Antibodies  >2 publications with HRK and Hepatitis
 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >21 publications with HRK and Brain
 Lymph Node Antibodies  >2 publications with HRK and Lymph Node
 Testis Antibodies  >1 publications with HRK and Testis
 Thyroid Antibodies  >1 publications with HRK and Thyroid
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