View our list of available Coronavirus (COVID-19, SARS-CoV-2, 2019-nCOV) antibodies, recombinant proteins, ELISA Kits... Options Full Datasheet Printer Friendly Datasheet Prepare for Printing Full Datasheet Printer Friendly Datasheet Prepare for Printing Have Questions? Ask Us! Request A Quote Today! Add To Compare List Rabbit I-kappa-Ba (Tyr-305) Polyclonal Antibody Catalog # MBS472027 Unit / Price 0.1 mL / $465 +1 FREE 8GB USB 5x0.1 mL / $1,920 +2 FREE 8GB USB Product Name Product Info Accession #s Product Desc Diseases/Tissues/Pathways Applications References Product Name I-kappa-Ba (Tyr-305), Polyclonal Antibody Full Product Name I-kappa-Ba (Tyr-305), phospho-specific Product Synonym Names NF-kappa-B inhibitor alpha; IKB-alpha; IkappaBalpha; Major histocompatibility complex enhancer-binding protein; MAD3; IkBa Research Use Only For Research Use Only. Not for use in diagnostic procedures. TOP Clonality Polyclonal Host Rabbit Species Reactivity Human, Rat, Mouse Specificity This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho- IkBa (Tyr-305) peptide (without carrier). The antibody detects a 38 kDa* protein on SDS-PAGE immunoblots of A431 and Jurkat cells treated with pervanadate, but does not detect this band in control cells. Purity/Purification Affinity Purified Form/Format Rabbit polyclonal, affinity-purified antibody is supplied in 100 ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Immunogen IkBa (Tyr-305) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 305 of human IkBa. This peptide sequence has low homology to other IkB proteins. Preparation and Storage Store at -20 degree C. Do not aliquot. Stable for 1 year. Other Notes Small volumes of anti-I-kappa-Ba (Tyr-305) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. TOP Related Product Information for anti-I-kappa-Ba (Tyr-305) antibody The NF-kB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IkB proteins. Activation of IkBa ?occurs through both serine and tyrosine phosphorylation events. Activation through phosphorylation at Ser-32 and Ser-36 is followed by proteasome-mediated degradation, resulting in the release and nuclear translocation of active NF-kB. This pathway of IkBa regulation occurs in response to various NF-kB-activating agents, such as TNF-alpha, interleukins, LPS, and irradiation. An alternative pathway for IkBa regulation occurs through tyrosine phosphorylation of Tyr-42 and Tyr-305. Tyr-42 is phosphorylated in response to oxidative stress and growth factors. This phosphorylation can lead to degradation of IkB-alpha and NF-kB-activation. In contrast, Tyr-305 phosphorylation by c-Abl has been implicated in IkB-alpha nuclear translocation and inhibition of NF-kB-activation. Thus, tyrosine phosphorylation of IkB-alpha may be an important regulatory mechanism in NF-kB signaling. Applications Tested/Suitable for anti-I-kappa-Ba (Tyr-305) antibody ELISA, Western Blot Application Notes for anti-I-kappa-Ba (Tyr-305) antibody ELISA: 1:2000Western Blot: 1:500 Testing Data of anti-I-kappa-Ba (Tyr-305) antibody TOP Product References and Citations for anti-I-kappa-Ba (Tyr-305) antibody • Bui, N.T. et al. (2001) J Cell Biol 152(4):753.• Finco, T.S. et al. (1994) Proc. Natl. Acad. Sci. USA 91:11884.• Waris et al. (2003) J Biol Chem 278(42):40778. TOP Precautions All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines. Disclaimer While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information. TOP