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anti-IRS1 antibody :: Rabbit anti-Mouse IRS-1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS624304
Unit / Price
0.1 mL  /  $615 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

IRS-1 (IRS1), Polyclonal Antibody

★Popular Item★
 Also Known As   

IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)

 Product Synonym Names    Anti -IRS-1, phosphorylated (Tyr612) (Insulin Receptor Substrate 1)
 Product Gene Name   

anti-IRS1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Chromosome Location    Chromosome: 2; NC_000002.11 (227596033..227663506, complement). Location: 2q36
 OMIM    125853
 3D Structure    ModBase 3D Structure for P35568
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Mouse
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 Specificity    Recognizes human IRS-1 when phosphorylated at Tyr612
 Purity/Purification    Affinity Purified
Purified by affinity chromatography.
 Form/Format    Supplied as a liquid in PBS, pH 7.3, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    Synthetic phosphopeptide derived from a region of human IRS-1 that contains tyrosine 612
 Species sequence homology    rat, chicken
 Positive Control    CHO-T cells transiently transfected with human IRS-1
    
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 Preparation and Storage    May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
 Other Notes    Small volumes of anti-IRS1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-IRS1 antibody

   Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood. IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm"e;s tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option. To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
 Product Categories/Family for anti-IRS1 antibody    Antibodies; Abs to Protein Kinase Substrates
 Applications Tested/Suitable for anti-IRS1 antibody   

Flow Cytometry (FC), Western Blot (WB), Immunohistochemistry (IHC). Other applications not tested.

 Application Notes for anti-IRS1 antibody    Recommended Dilution:
Immunohistochemistry (Paraffin): 1:10-1:100
Flow Cytometry: 3-5ug/million cells

Optimal dilutions to be determined by the researcher.
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 Western Blot of anti-IRS1 antibody    Western Blot analysis
anti-IRS1 antibody Western Blot (WB) image
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 Immunohistochemistry of anti-IRS1 antibody    Immunohistochemistry analysis of Phospho-IRS 1 [pY612] showing staining in the cytoplasm and nucleus of paraffin-embedded human skeletal muscle tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 1 OmM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H202-methanol for 15 min at RT, washed with ddH20 and PBS, and then probed with MBS624304 diluted in 3% BSA-PBS at a dilution of 1 :20 overnight at 4 degree C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
anti-IRS1 antibody Immunohistochemistry (IHC) image
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 Flow cytometry of anti-IRS1 antibody    Flow cytometry analysis of IRS 1 [pY612] was done on MCF7 cells treated with Insulin 100nM, 10 minutes). Cells were fixed with 70% ethanol for 10 minutes, permeabilized with 0.25% TritonTM X-1 00 for 20 minutes, and blocked with 5% BSA for 30 minutes at RT. Cells were labeled with MBS624304 (red histogram) or with rabbit isotype control (pink histogram) at 3-Sug/million cells in 2.5% BSA. After incubation at RT for 2 hours, the cells were labeled with Alexa FluorŪ 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1 :400 for 30 minutes at RT. The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.
anti-IRS1 antibody Flow cytometry image
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NCBI/Uniprot data below describe general gene information for IRS1. It may not necessarily be applicable to this product.
 NCBI GI #    5031805
 NCBI GeneID    3667
 NCBI Accession #    NP_005535.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_005544.2 [Other Products]
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 UniProt Primary Accession #    P35568 [Other Products]
 UniProt Related Accession #    P35568 [Other Products]
 Molecular Weight    131,591 Da [Similar Products]
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 NCBI Official Full Name    insulin receptor substrate 1
 NCBI Official Synonym Full Names    insulin receptor substrate 1
 NCBI Official Symbol    IRS1 [Similar Products]
 NCBI Official Synonym Symbols   
HIRS-1
[Similar Products]
 NCBI Protein Information    insulin receptor substrate 1; IRS-1; OTTHUMP00000164234
 UniProt Protein Name    Insulin receptor substrate 1
 Protein Family    Isoleucine--tRNA ligase
 UniProt Gene Name    IRS1 [Similar Products]
 UniProt Entry Name    IRS1_HUMAN
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 NCBI Summary for IRS1    This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq]
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 UniProt Comments for IRS1    IRS1: an adaptor protein that is one of the major substrates of the insulin receptor kinase. Contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain-containing proteins including phosphatidylinositol 3-kinase p85 subunit and GRB-2. Phosphorylated on ser/thr residues by kinases including JNK and IKK.

Protein type: Motility/polarity/chemotaxis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q36

Cellular Component: intracellular membrane-bound organelle; cytoplasm; plasma membrane; caveola; nucleus; cytosol

Molecular Function: insulin-like growth factor receptor binding; protein binding; signal transducer activity; protein kinase C binding; phosphoinositide 3-kinase binding; SH2 domain binding; transmembrane receptor protein tyrosine kinase adaptor protein activity; insulin receptor binding

Biological Process: response to peptide hormone stimulus; phosphoinositide 3-kinase cascade; nerve growth factor receptor signaling pathway; positive regulation of glycogen biosynthetic process; negative regulation of insulin receptor signaling pathway; signal transduction; glucose homeostasis; response to insulin stimulus; negative regulation of insulin secretion; positive regulation of glucose import; mammary gland development; positive regulation of mesenchymal cell proliferation; positive regulation of cell proliferation; protein kinase B signaling cascade; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; positive regulation of phosphoinositide 3-kinase activity; positive regulation of fatty acid beta-oxidation; insulin-like growth factor receptor signaling pathway; cellular response to insulin stimulus; regulation of gene expression; insulin receptor signaling pathway; innate immune response; positive regulation of insulin receptor signaling pathway; lipid catabolic process; positive regulation of cell migration

Disease: Diabetes Mellitus, Noninsulin-dependent
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 Research Articles on IRS1    1. miR-126 was actively involved in the development of insulin resistance induced by mitochondrial dysfunction.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IRS1 antibodyPathways associated with anti-IRS1 antibody
 Reference Product  PubMed Publications
 IGF1R antibody  >212 publications with IRS1 and IGF1R
 AKT1 antibody  >165 publications with IRS1 and AKT1
 GRB2 antibody  >136 publications with IRS1 and GRB2
 MTOR antibody  >107 publications with IRS1 and MTOR
 MAPK1 antibody  >73 publications with IRS1 and MAPK1
 SHC1 antibody  >64 publications with IRS1 and SHC1
 JAK2 antibody  >55 publications with IRS1 and JAK2
 PTPN11 antibody  >41 publications with IRS1 and PTPN11
 INSR antibody  >32 publications with IRS1 and INSR
 SOCS3 antibody  >30 publications with IRS1 and SOCS3
 Products by Pathway  Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipogenesis Pathway antibodies  Adipogenesis Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Aldosterone-regulated Sodium Reabsorption Pathway antibodies  Aldosterone-regulated Sodium Reabsorption Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Downstream Signaling Of Activated FGFR Pathway antibodies  Downstream Signaling Of Activated FGFR Pathway Diagram
 EPO Receptor Signaling Pathway antibodies  EPO Receptor Signaling Pathway Diagram
 IGF1 Pathway antibodies  IGF1 Pathway Diagram
 IL-2 Signaling Pathway antibodies  IL-2 Signaling Pathway Diagram
Diseases associated with anti-IRS1 antibodyOrgans/Tissues associated with anti-IRS1 antibody
 Disease Name  Pubmed Publications
 Insulin Resistance Antibodies  >920 publications with IRS1 and Insulin Resistance
 Hyperinsulinism Antibodies  >622 publications with IRS1 and Hyperinsulinism
 Neoplasms Antibodies  >310 publications with IRS1 and Neoplasms
 Obesity Antibodies  >286 publications with IRS1 and Obesity
 Diabetes Mellitus, Type 2 Antibodies  >254 publications with IRS1 and Diabetes Mellitus, Type 2
 Necrosis Antibodies  >132 publications with IRS1 and Necrosis
 Breast Neoplasms Antibodies  >78 publications with IRS1 and Breast Neoplasms
 Inflammation Antibodies  >64 publications with IRS1 and Inflammation
 Hypertension Antibodies  >64 publications with IRS1 and Hypertension
 Fatty Liver Antibodies  >63 publications with IRS1 and Fatty Liver
 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >698 publications with IRS1 and Muscle
 Liver Antibodies  >362 publications with IRS1 and Liver
 Connective Tissue Antibodies  >200 publications with IRS1 and Connective Tissue
 Pancreas Antibodies  >124 publications with IRS1 and Pancreas
 Ovary Antibodies  >98 publications with IRS1 and Ovary
 Brain Antibodies  >84 publications with IRS1 and Brain
 Heart Antibodies  >83 publications with IRS1 and Heart
 Kidney Antibodies  >82 publications with IRS1 and Kidney
 Nerve Antibodies  >37 publications with IRS1 and Nerve
 Bone Antibodies  >33 publications with IRS1 and Bone
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