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anti-ITGA6 antibody :: Rabbit ITGA6 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9203054
Unit / Price
0.08 mL  /  $155 +1 FREE 8GB USB
0.4 mL  /  $340 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

ITGA6, Polyclonal Antibody

 Also Known As   

ITGA6 Antibody (isoform 2 S1064)

 Product Synonym Names    Integrin alpha-6; CD49 antigen-like family member F; VLA-6; CD49f; Integrin alpha-6 heavy chain; Integrin alpha-6 light chain; Processed integrin alpha-6; Alpha6p; ITGA6
 Product Gene Name   

anti-ITGA6 antibody

[Similar Products]
 Antibody/Peptide Pairs    ITGA6 (isoform 2 S1064) peptide (MBS9224796) is used for blocking the activity of ITGA6 antibody (MBS9203054)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 Immunogen Sequence Positions    1043-1072
 OMIM    147556
 3D Structure    ModBase 3D Structure for P23229
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 Clonality    Polyclonal
 Isotype    Rabbit Ig
 Host    Rabbit
 Species Reactivity    Human (Predicted Reactivity: Chicken)
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 Specificity    This ITGA6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1043-1072 amino acids from human ITGA6.
 Purity/Purification    Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
 Form/Format    Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
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 Preparation and Storage    Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
 Other Notes    Small volumes of anti-ITGA6 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Product Description specifically for anti-ITGA6 antibody

   The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling.
 Product Categories/Family for anti-ITGA6 antibody    Developmental Biology; Signal Transduction
 Applications Tested/Suitable for anti-ITGA6 antibody   

WB, IF, IHC-P, ELISA

 Application Notes for anti-ITGA6 antibody    IF~~1:200
WB~~1:1000
IHC-P~~1:10~50
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 Western Blot of anti-ITGA6 antibody    Anti-ITGA6 Antibody (isoform 2 S1064) at 1:1000 dilution + SW480 whole cell lysate Lysates/proteins at 20 ?g per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 119 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
anti-ITGA6 antibody Western Blot (WB) image
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 Immunofluorescence (IF) of anti-ITGA6 antibody    Fluorescent confocal image of HeLa cells stained with ITGA6 (isoform 2 S1064) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with MBS9203054 ITGA6 (isoform 2 S1064) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the ITGA6 mainly to the nucleus, supported by Human Protein Atlas Data (http://www.proteinatlas.org/ENSG00000091409).
anti-ITGA6 antibody Immunofluorescence (IF) image
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 Western Blot (WB) of anti-ITGA6 antibody    Western blot analysis of anti-ITGA6 Antibody (isoform 2 S1064) in 293 cell line lysates (35ug/lane). ITGA6(arrow) was detected using the purified Pab.
anti-ITGA6 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-ITGA6 antibody    Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with ITGA6 Antibody (isoform 2 S1064) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
anti-ITGA6 antibody Immunohistochemistry (IHC) (IHC) image
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NCBI/Uniprot data below describe general gene information for ITGA6. It may not necessarily be applicable to this product.
 NCBI GI #    119395742
 NCBI GeneID    3655
 NCBI Accession #    NP_000201.2 [Other Products]
 NCBI Related Accession #    NP_001073286.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_000210.2 [Other Products]
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 UniProt Primary Accession #    P23229 [Other Products]
 UniProt Secondary Accession #    Q08443; Q0MRC7; Q14646; Q16508; Q53RX7; Q59HB7; B2RMU9; B4DG69; B4DKB8; C4AM96; G5E9H1 [Other Products]
 UniProt Related Accession #    P23229 [Other Products]
 Molecular Weight    126606
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 NCBI Official Full Name    integrin alpha-6 isoform b
 NCBI Official Synonym Full Names    integrin, alpha 6
 NCBI Official Symbol    ITGA6 [Similar Products]
 NCBI Official Synonym Symbols   
CD49f; VLA-6; ITGA6B
[Similar Products]
 NCBI Protein Information    integrin alpha-6
 UniProt Protein Name    Integrin alpha-6
 UniProt Synonym Protein Names   
CD49 antigen-like family member F; VLA-6; CD_antigen: CD49f
 Protein Family    Integrin
 UniProt Gene Name    ITGA6 [Similar Products]
 UniProt Synonym Gene Names    Alpha6p [Similar Products]
 UniProt Entry Name    ITA6_HUMAN
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 NCBI Summary for ITGA6    The ITGA6 protein product is the integrin alpha chain alpha 6. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signalling. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
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 UniProt Comments for ITGA6    ITGA6: Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. Alpha-6 associates with either beta-1 or beta-4. Interacts with HPS5. Interacts with RAB21. Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). Belongs to the integrin alpha chain family. 8 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Receptor, misc.; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cell-cell adherens junction; cell surface; focal adhesion; hemidesmosome; basal plasma membrane; plasma membrane; basement membrane; filopodium; external side of plasma membrane

Molecular Function: integrin binding; protein binding; metal ion binding; laminin binding

Biological Process: integrin-mediated signaling pathway; skin development; extracellular matrix organization and biogenesis; filopodium formation; positive regulation of apoptosis; cell-matrix adhesion; cell-substrate adhesion; cell-cell adhesion; hemidesmosome assembly; gut development; cellular response to extracellular stimulus; cell-substrate junction assembly; brown fat cell differentiation; positive regulation of cell-cell adhesion; cell adhesion mediated by integrin; positive regulation of transcription from RNA polymerase II promoter; blood coagulation; leukocyte migration; positive regulation of phosphorylation

Disease: Epidermolysis Bullosa Junctionalis With Pyloric Atresia
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Product References and Citations for anti-ITGA6 antibody

   References for protein:
1.Yang,X.H., Cancer Res. 68 (9), 3204-3213 (2008)
2.Hayashi,R., Biochem. Biophys. Res. Commun. 367 (2), 256-263 (2008)

References for HeLa cell line:
1. Scherer WF, Syverton JT, Gey GO (May 1953). "Studies on the propagation in vitro of poliomyelitis viruses. IV. Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix". J. Exp. Med. 97 (5): 695-710. [PubMed:13052828].

2. Macville M, Schröck E, Padilla-Nash H, Keck C, Ghadimi BM, Zimonjic D, Popescu N, Ried T (January 1999). "Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping". Cancer Res. 59 (1): 141-50. [PubMed: 9892199].
3. Rahbari R, Sheahan T, Modes V, Collier P, Macfarlane C, Badge RM (April 2009). "A novel L1 retrotransposon marker for HeLa cell line identification". BioTechniques 46 (4): 277-84. [PubMed: 19450234].

4. Capes-Davis A, Theodosopoulos G, Atkin I, Drexler HG, Kohara A, MacLeod RA, Masters JR, Nakamura Y, Reid YA, Reddel RR, Freshney RI (July 2010). "Check your cultures! A list of cross-contaminated or misidentified cell lines". Int. J. Cancer 127 (1): 1-8. [PubMed:20143388].
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 Research Articles on ITGA6    1. These results indicate that mAbs to the laminin a4 globular domain are able to inhibit tumor cell adhesion and migration on laminins 411 and 421, and that alpha6beta1 integrin and MCAM bind a4-laminins at very close sites on the globular domain
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-ITGA6 antibodyPathways associated with anti-ITGA6 antibody
 Reference Product  PubMed Publications
 ITGB4 antibody  >22 publications with ITGA6 and ITGB4
 ITGB1 antibody  >13 publications with ITGA6 and ITGB1
 ITGB5 antibody  >5 publications with ITGA6 and ITGB5
 CD9 antibody  >3 publications with ITGA6 and CD9
 LAMA5 antibody  >1 publications with ITGA6 and LAMA5
 COL17A1 antibody  >1 publications with ITGA6 and COL17A1
 PLEC antibody  >1 publications with ITGA6 and PLEC
 Products by Pathway  Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
 RNA Polymerase Pathway antibodies  RNA Polymerase Pathway Diagram
Diseases associated with anti-ITGA6 antibodyOrgans/Tissues associated with anti-ITGA6 antibody
 Disease Name  Pubmed Publications
 Epidermolysis bullosa with pyloric atresia Antibodies  >11 publications with ITGA6 and Epidermolysis bullosa with pyloric atresia
 Breast Neoplasms Antibodies  >9 publications with ITGA6 and Breast Neoplasms
 Neoplasm Metastasis Antibodies  >7 publications with ITGA6 and Neoplasm Metastasis
 Fibrosis Antibodies  >5 publications with ITGA6 and Fibrosis
 Prostatic Diseases Antibodies  >5 publications with ITGA6 and Prostatic Diseases
 Inflammation Antibodies  >5 publications with ITGA6 and Inflammation
 Neoplasm Invasiveness Antibodies  >4 publications with ITGA6 and Neoplasm Invasiveness
 Prostatic Neoplasms Antibodies  >4 publications with ITGA6 and Prostatic Neoplasms
 Liver Neoplasms Antibodies  >3 publications with ITGA6 and Liver Neoplasms
 Skin Neoplasms Antibodies  >3 publications with ITGA6 and Skin Neoplasms
 Organ/Tissue Name  Pubmed Publications
 Skin Antibodies  >22 publications with ITGA6 and Skin
 Blood Antibodies  >12 publications with ITGA6 and Blood
 Testis Antibodies  >12 publications with ITGA6 and Testis
 Stomach Antibodies  >10 publications with ITGA6 and Stomach
 Prostate Antibodies  >10 publications with ITGA6 and Prostate
 Bone Antibodies  >8 publications with ITGA6 and Bone
 Vascular Antibodies  >5 publications with ITGA6 and Vascular
 Liver Antibodies  >5 publications with ITGA6 and Liver
 Brain Antibodies  >5 publications with ITGA6 and Brain
 Mouth Antibodies  >4 publications with ITGA6 and Mouth
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