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anti-IFNG antibody :: Rabbit anti-Human Interferon gamma Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS175484
Western Blot (WB)
Unit / Price
0.1 mg  /  $280 +1 FREE 8GB USB
 
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 Product Name   

Interferon gamma (IFNG), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-human Interferon gamma antibody

 Product Synonym Names    Interferon gamma(IFN-gamma); Interferon gamma; IFG antibody; IFI antibody; IFN Gamma antibody; IFN Immune antibody; IFN-gamma antibody; IFNG antibody; Immune Interferon antibody; Interferon gamma antibody; Interferon Gamma Precursor antibody; Macrophage Activating Factor antibody; MAF antibody; T Cell Interferon antibody; Type II Interferon antibody
 Product Gene Name   

anti-IFNG antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    gene 609135
 3D Structure    ModBase 3D Structure for P01579
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 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human
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 Purity/Purification    Immunogen affinity purified.
 Form/Format    Lyophilized
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 Immunogen    E Coli-derived human IFNgamma recombinant protein (Position: Q24-Q166).
 Contents    Each vial contains 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
 Reconstitution    Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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 Preparation and Storage    At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
 ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.
 Other Notes    Small volumes of anti-IFNG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-IFNG antibody

   Description: Rabbit IgG polyclonal antibody for Interferon gamma(IFNG) detection. Tested with WB, IHC-P, ELISA, Neu, IP in Human.
Background: Interferon-gamma(IFN-gamma) is an inflammatory cytokine that has been implicated in the development of fibrosis in inflamed tissues. The production of IFN-gamma, which is under genetic control, can influence the development of fibrosis in lung allografts. IFN-gamma is also produced by natural killer(NK) cells and most prominently by CD8 cytotoxic T cells, and is vital for the control of microbial pathogens. Interferon gamma is believed to be crucial for host defence against many infections. Genetically determined variability in IFN-gamma and expression might be important for the development of tuberculosis. IFN-gamma activates human macrophage oxidative metabolism and antimicrobial activity. In addition to having antiviral activity, IFN-gamma has important immunoregulatory functions. IFN-gamma plays an important role in the control of neointima proliferation.
 Applications Tested/Suitable for anti-IFNG antibody   

Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Neu, Immunoprecipitation (IP)

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 Western Blot (WB) of anti-IFNG antibody    Figure. Western blot analysis of IFN gamma using anti- IFN gamma antibody (MBS175484).

Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.

Lane: Recombinant Human IFN gamma Protein 0.5ng

After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- IFN gamma antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IFN gamma at approximately 17KD. The expected band size for IFN gamma is at 17KD.
anti-IFNG antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for IFNG. It may not necessarily be applicable to this product.
 NCBI GI #    124479
 NCBI GeneID    3458
 NCBI Accession #    P01579.1 [Other Products]
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 UniProt Primary Accession #    P01579 [Other Products]
 UniProt Secondary Accession #    Q53ZV4; B5BU88 [Other Products]
 UniProt Related Accession #    P01579 [Other Products]
 Molecular Weight    19,348 Da
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 NCBI Official Full Name    Interferon gamma
 NCBI Official Synonym Full Names    interferon, gamma
 NCBI Official Symbol    IFNG [Similar Products]
 NCBI Official Synonym Symbols   
IFG; IFI
[Similar Products]
 NCBI Protein Information    interferon gamma; IFN-gamma; immune interferon
 UniProt Protein Name    Interferon gamma
 UniProt Synonym Protein Names   
Immune interferon
 Protein Family    Interferon
 UniProt Gene Name    IFNG [Similar Products]
 UniProt Synonym Gene Names    IFN-gamma [Similar Products]
 UniProt Entry Name    IFNG_HUMAN
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 NCBI Summary for IFNG    This gene encodes a member of the type II interferon family. The protein encoded is a soluble cytokine with antiviral, immunoregulatory and anti-tumor properties and is a potent activator of macrophages. Mutations in this gene are associated with aplastic anemia.[provided by RefSeq, Nov 2009]
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 UniProt Comments for IFNG    IFNG: Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. Homodimer. Released primarily from activated T lymphocytes. Belongs to the type II (or gamma) interferon family.

Protein type: Membrane protein, integral; Secreted; Secreted, signal peptide; Cytokine

Chromosomal Location of Human Ortholog: 12q14

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: interferon-gamma receptor binding; cytokine activity

Biological Process: positive regulation of isotype switching to IgG isotypes; positive regulation of nitric oxide biosynthetic process; apoptosis; positive regulation of interleukin-23 production; negative regulation of smooth muscle cell proliferation; positive regulation of interleukin-12 production; positive regulation of osteoclast differentiation; positive regulation of interleukin-6 biosynthetic process; negative regulation of epithelial cell differentiation; positive regulation of interleukin-1 beta secretion; positive regulation of killing of cells of another organism; negative regulation of transcription from RNA polymerase II promoter; sensory perception of mechanical stimulus; positive regulation of membrane protein ectodomain proteolysis; cell surface receptor linked signal transduction; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of T cell proliferation; cell cycle arrest; defense response to virus; regulation of the force of heart contraction; positive regulation of peptidyl-serine phosphorylation of STAT protein; response to drug; positive regulation of synaptic transmission, cholinergic; neutrophil chemotaxis; adaptive immune response; CD8-positive, alpha-beta T cell differentiation during immune response; negative regulation of myelination; unfolded protein response; response to virus; cytokine and chemokine mediated signaling pathway; defense response to protozoan; positive regulation of tumor necrosis factor production; humoral immune response; antigen processing and presentation; positive regulation of tyrosine phosphorylation of Stat1 protein; positive regulation of chemokine biosynthetic process; negative regulation of interleukin-17 production; positive regulation of interleukin-12 biosynthetic process; protein import into nucleus, translocation; defense response to bacterium; neutrophil apoptosis; positive regulation of transcription from RNA polymerase II promoter; cell motility; positive regulation of neuron differentiation; regulation of insulin secretion

Disease: Hepatitis C Virus, Susceptibility To; Aplastic Anemia; Tuberous Sclerosis 2; Mycobacterium Tuberculosis, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To
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 Research Articles on IFNG    1. CASP has a direct role in the secretion of IFN-gamma, and NK cell motility and ability to kill tumor cells. CASP polarizes to the leading edge of migrating NK cells, and to the immunological synapse when engaged with tumor cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-IFNG antibodyPathways associated with anti-IFNG antibody
 Reference Product  PubMed Publications
 CD4 antibody  >148 publications with IFNG and CD4
 IL2 antibody  >52 publications with IFNG and IL2
 TBX21 antibody  >51 publications with IFNG and TBX21
 STAT1 antibody  >37 publications with IFNG and STAT1
 IFNGR1 antibody  >29 publications with IFNG and IFNGR1
 IL18 antibody  >23 publications with IFNG and IL18
 IRF1 antibody  >16 publications with IFNG and IRF1
 CD86 antibody  >5 publications with IFNG and CD86
 Products by Pathway  Pathway Diagram
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 African Trypanosomiasis Pathway antibodies  African Trypanosomiasis Pathway Diagram
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 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Allograft Rejection Pathway antibodies  Allograft Rejection Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Amoebiasis Pathway antibodies  Amoebiasis Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
 Antigen Processing And Presentation Pathway antibodies  Antigen Processing And Presentation Pathway Diagram
Diseases associated with anti-IFNG antibodyOrgans/Tissues associated with anti-IFNG antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >142 publications with IFNG and Necrosis
 Autoimmune Diseases Antibodies  >85 publications with IFNG and Autoimmune Diseases
 Respiratory Tract Diseases Antibodies  >66 publications with IFNG and Respiratory Tract Diseases
 Hepatitis Antibodies  >32 publications with IFNG and Hepatitis
 Lymphoproliferative Disorders Antibodies  >20 publications with IFNG and Lymphoproliferative Disorders
 Diabetes Mellitus, Type 1 Antibodies  >17 publications with IFNG and Diabetes Mellitus, Type 1
 HIV Infections Antibodies  >16 publications with IFNG and HIV Infections
 Arthritis, Rheumatoid Antibodies  >15 publications with IFNG and Arthritis, Rheumatoid
 Hepatitis C Antibodies  >14 publications with IFNG and Hepatitis C
 Leishmaniasis Antibodies  >9 publications with IFNG and Leishmaniasis
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >455 publications with IFNG and Blood
 Kidney Antibodies  >40 publications with IFNG and Kidney
 Lymph Node Antibodies  >24 publications with IFNG and Lymph Node
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