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anti-MAPK8 antibody :: Rabbit JNK1/2/3 (pT183) Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS821367
Unit / Price
0.03 mL  /  $165 +1 FREE 8GB USB
0.1 mL  /  $245 +1 FREE 8GB USB
0.2 mL  /  $350 +1 FREE 8GB USB
 
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 Product Name   

JNK1/2/3 (pT183) (MAPK8), Polyclonal Antibody

★Popular Item★
 Also Known As   

Anti-JNK1/2/3 (pT183) Antibody

 Product Synonym Names    MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mitogen-activated protein kinase 9; MAP kinase 9; JNK-55; Stress-activated protein kinase 1a; SAPK1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK10; JNK3; JNK3A; PRKM10; SAPK1B; Mitogen-activated protein kinase 10; MAP kinase 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3
 Product Gene Name   

anti-MAPK8 antibody

[Similar Products]
 Product Synonym Gene Name    MAPK9; MAPK10 [Similar Products]
 Antibody/Peptide Pairs    JNK1/2/3 (pT183) peptide (MBS823658) is used for blocking the activity of JNK1/2/3 (pT183) antibody (MBS821367)
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 OMIM    601158
 3D Structure    ModBase 3D Structure for P45983
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 Clonality    Polyclonal
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat, Bovine, Chicken, Pig
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 Specificity    Recognizes endogenous levels of JNK1/2/3 (pT183) protein.
 Purity/Purification    The antibody was purified by immunogen affinity chromatography.
 Form/Format    Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
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 Gene Symbol    MAPK8; MAPK9; MAPK10
 Entrez Gene    5599, 5601, 5602 (Human)
26419, 26420 (Mouse)
116554,50658, 25272 (Rat)
 Swiss Prot    P45983, P45984, P53779 (Human)
Q91Y86, Q9WTU6, Q61831 (Mouse)
P49185, P49186, P49187 (Rat)
 Immunogen    KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JNK1/2/3. The exact sequence is proprietary.
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 Preparation and Storage    Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
 Other Notes    Small volumes of anti-MAPK8 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-MAPK8 antibody

   Rabbit polyclonal antibody to JNK1/2/3 (pT183)
 Applications Tested/Suitable for anti-MAPK8 antibody   

WB, IH, IF/IC

 Application Notes for anti-MAPK8 antibody    WB (1/500 - 1/1000)
IH (1/100 - 1/200)
IF/IC (1/100 - 1/500)
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 Western Blot (WB) of anti-MAPK8 antibody    Western blot analysis of JNK1/2/3 (pT183) expression in MCF7 PMA-treated (A), NIH3T3 UV-treated (B), PC12 PMA-treated (C) whole cell lysates
anti-MAPK8 antibody Western Blot (WB) (WB) image
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 Immunohistochemistry (IHC) of anti-MAPK8 antibody    Immunohistochemical analysis of JNK1/2/3 (pT183) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
anti-MAPK8 antibody Immunohistochemistry (IHC) (IHC) image
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 Immunofluorescence/Immunocytochemistry (IF/ICC) of anti-MAPK8 antibody    Immunofluorescent analysis of JNK1/2/3 (pT183) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
anti-MAPK8 antibody Immunofluorescence/Immunocytochemistry (IF/ICC) (ICC) image
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NCBI/Uniprot data below describe general gene information for MAPK8. It may not necessarily be applicable to this product.
 NCBI GI #    513788279
 NCBI GeneID    5599
 NCBI Accession #    NP_001265476.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_001278547.1 [Other Products]
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 UniProt Primary Accession #    P45983 [Other Products]
 UniProt Secondary Accession #    Q15709; Q15712; Q15713; Q308M2; B5BTZ5; B7ZLV4; D3DX88; D3DX92 [Other Products]
 UniProt Related Accession #    P45983 [Other Products]
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 NCBI Official Full Name    mitogen-activated protein kinase 8 JNK1 beta2
 NCBI Official Synonym Full Names    mitogen-activated protein kinase 8
 NCBI Official Symbol    MAPK8 [Similar Products]
 NCBI Official Synonym Symbols   
JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
[Similar Products]
 NCBI Protein Information    mitogen-activated protein kinase 8; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8 isoform JNK1 alpha1
 UniProt Protein Name    Mitogen-activated protein kinase 8
 UniProt Synonym Protein Names   
JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1
 UniProt Gene Name    MAPK8 [Similar Products]
 UniProt Synonym Gene Names    JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c [Similar Products]
 UniProt Entry Name    MK08_HUMAN
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 NCBI Summary for MAPK8    The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]
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 UniProt Comments for MAPK8    JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: nucleoplasm; mitochondrion; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; enzyme binding; histone deacetylase binding; JUN kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of protein localization; regulation of transcription factor activity; JNK cascade; response to stress; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; regulation of histone deacetylation; ossification; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of circadian rhythm; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis
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 Research Articles on MAPK8    1. These findings suggest for the first time that FOXO1 is a promising candidate substrate for JNK, and the FOXO1-dependent DNA damage repair may be regulated positively by the JNK pathway in H1299 lung cancer cells.
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-MAPK8 antibodyPathways associated with anti-MAPK8 antibody
 Reference Product  PubMed Publications
 JUN antibody  >814 publications with MAPK8 and JUN
 FOS antibody  >43 publications with MAPK8 and FOS
 MAP2K4 antibody  >29 publications with MAPK8 and MAP2K4
 IRS1 antibody  >23 publications with MAPK8 and IRS1
 ATF2 antibody  >22 publications with MAPK8 and ATF2
 MAPK8IP1 antibody  >13 publications with MAPK8 and MAPK8IP1
 JUND antibody  >12 publications with MAPK8 and JUND
 TP53 antibody  >7 publications with MAPK8 and TP53
 JUNB antibody  >6 publications with MAPK8 and JUNB
 MAPK8IP3 antibody  >4 publications with MAPK8 and MAPK8IP3
 Products by Pathway  Pathway Diagram
 AGE/RAGE Pathway antibodies  AGE/RAGE Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Activated TLR4 Signalling Pathway antibodies  Activated TLR4 Signalling Pathway Diagram
 Activation Of BH3-only Proteins Pathway antibodies  Activation Of BH3-only Proteins Pathway Diagram
 Activation Of BIM And Translocation To Mitochondria Pathway antibodies  Activation Of BIM And Translocation To Mitochondria Pathway Diagram
 Activation Of BMF And Translocation To Mitochondria Pathway antibodies  Activation Of BMF And Translocation To Mitochondria Pathway Diagram
 Activation Of The AP-1 Family Of Transcription Factors Pathway antibodies  Activation Of The AP-1 Family Of Transcription Factors Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Adipocytokine Signaling Pathway antibodies  Adipocytokine Signaling Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
Diseases associated with anti-MAPK8 antibodyOrgans/Tissues associated with anti-MAPK8 antibody
 Disease Name  Pubmed Publications
 Necrosis Antibodies  >162 publications with MAPK8 and Necrosis
 Inflammation Antibodies  >124 publications with MAPK8 and Inflammation
 Nervous System Diseases Antibodies  >82 publications with MAPK8 and Nervous System Diseases
 Disease Models, Animal Antibodies  >65 publications with MAPK8 and Disease Models, Animal
 Liver Diseases Antibodies  >59 publications with MAPK8 and Liver Diseases
 Brain Diseases Antibodies  >56 publications with MAPK8 and Brain Diseases
 Reperfusion Injury Antibodies  >36 publications with MAPK8 and Reperfusion Injury
 Cell Transformation, Neoplastic Antibodies  >36 publications with MAPK8 and Cell Transformation, Neoplastic
 Breast Neoplasms Antibodies  >35 publications with MAPK8 and Breast Neoplasms
 Heart Diseases Antibodies  >34 publications with MAPK8 and Heart Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >159 publications with MAPK8 and Blood
 Brain Antibodies  >140 publications with MAPK8 and Brain
 Vascular Antibodies  >88 publications with MAPK8 and Vascular
 Lung Antibodies  >70 publications with MAPK8 and Lung
 Heart Antibodies  >68 publications with MAPK8 and Heart
 Bone Antibodies  >38 publications with MAPK8 and Bone
 Skin Antibodies  >37 publications with MAPK8 and Skin
 Kidney Antibodies  >36 publications with MAPK8 and Kidney
 Connective Tissue Antibodies  >29 publications with MAPK8 and Connective Tissue
 Prostate Antibodies  >27 publications with MAPK8 and Prostate
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