Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource rightarrow Antibody rightarrow KEAP1 rightarrow Polyclonal KEAP1  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Menu separator
separator
ELISA Kit ELISA Kit
CLIA Kit CLIA Kit
PCR Kit PCR Kit
Monoclonal Antibody Monoclonal Antibody
Polyclonal Antibody Polyclonal Antibody
Secondary Antibody Secondary Antibody
Antigen Antigen
Biochemical Biochemical
cDNA Clone cDNA Clone
siRNA siRNA
Peptide Peptide
Recombinant/Purified Protein Rec./Purified Protein

Custom ELISA Kit Custom ELISA Kit
Custom Protein Custom Protein
Custom Antibody Custom Antibody
Antibody Matched Pairs Antibody Matched Pairs
Antibody & Corresponding Blocking Peptide Pairs Antibody Peptide Pairs
Phospho-Specific Antibodies Phospho Antibodies
Products by Disease Products by Disease
Products by Pathway Products by Pathway
Products by Tissue Products by Tissue

arrow Advanced Search
arrow Submit Technical Q&A
arrow International Distributors
arrow Contact Us
separator
Our Best Sellers moreseparator
separator
 • MYH9 Antibody
 • GFM2 Antibody
 • Clara cell protein, CC16 ELISA Kit
 • CNN2 Antibody
 • alpha 1-Microglobulin (AMBP) Antibody
 • PHOSPHORYLASE B Purified Protein
 • Platelet membrane glycoprotein IIb (GPIIb) (Itga2b) ELISA Kit
 • Lactate Dehydrogenase Total (Ldh) Protein
 • Lipoxin A4 (LXA4) ELISA Kit
 • Glucose 6 Phosphate (G6P) ELISA Kit
 • LIFR ELISA Kit
 • Barrier-to-autointegration factor (BANF1) Recombinant Protein
 • Neutrophil Elastase ELISA Kit
 • CAMK4 Antibody
 downarrow more ...
separator
separator
DatasheetFull DatasheetPrinter Friendly DatasheetPrint This DatasheetAdd to Compare ListHave Questions? Ask UsRequest for a Quotation today

anti-KEAP1 antibody :: Rabbit KEAP1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9607293
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

KEAP1, Polyclonal Antibody

★Popular Item★
 Also Known As   

KEAP1 Antibody

 Product Synonym Names    Cytosolic inhibitor of Nrf2; INrf 2; INrf2; Keap 1; KEAP1; KEAP1_HUMAN; Kelch like ECH associated protein 1; Kelch like family member 19; Kelch like protein 19; Kelch-like ECH-associated protein 1; Kelch-like protein 19; KIAA0132; KLHL 19; KLHL19; MGC10630; MGC1114; MGC20887; MGC4407; MGC9454
 Product Gene Name   

anti-KEAP1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar
 3D Structure    ModBase 3D Structure for Q14145
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse, Rat
Section Bar
 Specificity    KEAP1 antibody detects endogenous levels of total KEAP1
 Purity/Purification    The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
Section Bar
 Immunogen    A synthesized peptide derived from human KEAP1
 Subcellular Location    Cytoplasm. Nucleus. Shuttles between cytoplasm and nucleus.
 Tissue Specificity    Broadly expressed, with highest levels in skeletal muscle.
 Conjugation    Unconjugated
Section Bar
 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-KEAP1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Table BarTOPTable Bar
 

Related Product Information for anti-KEAP1 antibody

   Description: The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5). The NRF2 repressor KEAP1 contains an amino terminal BTB/POZ domain and a carboxyl terminal KELCH domain (6, 7). The KELCH domain is required for interacting with NRF2 and the BTB/POZ domain functions in binding Cul3 E3 ubiquitin ligase (8-10). Under normal conditions, the complex leads to the cytoplasmic sequestration and ubiquitin-mediated proteasomal degradation of NRF2. Electrophilic modification of KEAP1 leads to disassociation of the NRF2/KEAP1 complex. KEAP1 also targets the down regulation of NF-kappaB activity by targeting IKKbeta degradation (11).
Function: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Subunit Structure: Homodimer. Forms a ternary complex with NFE2L2 and PGAM5. Interacts with the N-terminal regulatory domain of NFE2L2/NRF2. Interacts with BPTF and PTMA. Interacts with CUL3. Part of a complex that contains KEAP1, CUL3 and RBX1. Interacts with NFE2L1 and MAP1LC3B. Interacts indirectly with ENC1. Interacts with SESN1 and SESN2 (PubMed:23274085). Interacts with HSP90AA1 and HSP90AB1 (PubMed:26517842).
Post-translational Modifications: Ubiquitinated by the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and is subject to proteasomal-independent degradation. Quinone-induced oxidative stress, but not sulforaphane, increases its ubiquitination. Ubiquitination and subsequent degradation is most pronounced following prolonged exposure of cells to oxidative stress, particularly in glutathione-deficient cells that are highly susceptible to oxidative stress.
Similarity: The Kelch repeats mediate interaction with NF2L2/NRF2, BPTF and PGAM5.
 Applications Tested/Suitable for anti-KEAP1 antibody   

Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)

 Application Notes for anti-KEAP1 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
Section Bar
 Western Blot (WB) of anti-KEAP1 antibody    Western blot analysis of extracts of HepG2, using KEAP1 antibody. The lane on the left is treated with the antigen-specific peptide.
anti-KEAP1 antibody Western Blot (WB) (WB) image
Section Bar
 Immunohistochemistry (IHC) of anti-KEAP1 antibody    MBS9607293 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-KEAP1 antibody Immunohistochemistry (IHC) (IHC) image
Table BarTOPTable Bar
NCBI/Uniprot data below describe general gene information for KEAP1. It may not necessarily be applicable to this product.
 NCBI GI #    22027642
 NCBI GeneID    9817
 NCBI Accession #    NP_036421.2 [Other Products]
 NCBI GenBank Nucleotide #    NM_012289.3 [Other Products]
Section Bar
 UniProt Primary Accession #    Q14145 [Other Products]
 UniProt Secondary Accession #    Q6LEP0; Q8WTX1; Q9BPY9; B3KPD5 [Other Products]
 UniProt Related Accession #    Q14145 [Other Products]
 Molecular Weight    Observed: 70 kDa
Predicted: 70 kDa
Table BarTOPTable Bar
 NCBI Official Full Name    kelch-like ECH-associated protein 1
 NCBI Official Synonym Full Names    kelch like ECH associated protein 1
 NCBI Official Symbol    KEAP1 [Similar Products]
 NCBI Official Synonym Symbols   
INrf2; KLHL19
[Similar Products]
 NCBI Protein Information    kelch-like ECH-associated protein 1
 UniProt Protein Name    Kelch-like ECH-associated protein 1
 UniProt Synonym Protein Names   
Cytosolic inhibitor of Nrf2; INrf2; Kelch-like protein 19
 Protein Family    Kelch-like ECH-associated protein
 UniProt Gene Name    KEAP1 [Similar Products]
 UniProt Synonym Gene Names    INRF2; KIAA0132; KLHL19; INrf2 [Similar Products]
Section Bar
 NCBI Summary for KEAP1    This gene encodes a protein containing KELCH-1 like domains, as well as a BTB/POZ domain. Kelch-like ECH-associated protein 1 interacts with NF-E2-related factor 2 in a redox-sensitive manner and the dissociation of the proteins in the cytoplasm is followed by transportation of NF-E2-related factor 2 to the nucleus. This interaction results in the expression of the catalytic subunit of gamma-glutamylcysteine synthetase. Two alternatively spliced transcript variants encoding the same isoform have been found for this gene. [provided by RefSeq, Jul 2008]
Section Bar
 UniProt Comments for KEAP1    Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
Table BarTOPTable Bar
 Research Articles on KEAP1    1. this study shows changes of KEAP1 expression levels induced by cell-free DNA in different cell types
Table BarTOPTable Bar
 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Table BarTOPTable Bar
Products associated with anti-KEAP1 antibodyPathways associated with anti-KEAP1 antibody
 Reference Product  PubMed Publications
 NFE2L2 antibody  >1068 publications with KEAP1 and NFE2L2
 CUL3 antibody  >107 publications with KEAP1 and CUL3
 SQSTM1 antibody  >54 publications with KEAP1 and SQSTM1
 RBX1 antibody  >38 publications with KEAP1 and RBX1
 IKBKB antibody  >9 publications with KEAP1 and IKBKB
 PGAM5 antibody  >6 publications with KEAP1 and PGAM5
 UBC antibody  >3 publications with KEAP1 and UBC
 NFE2L1 antibody  >2 publications with KEAP1 and NFE2L1
 DPP3 antibody  >1 publications with KEAP1 and DPP3
 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 Antigen Processing: Ubiquitination & Proteasome Degradation Pathway antibodies  Antigen Processing: Ubiquitination & Proteasome Degradation Pathway Diagram
 Class I MHC Mediated Antigen Processing & Presentation Pathway antibodies  Class I MHC Mediated Antigen Processing & Presentation Pathway Diagram
 Immune System Pathway antibodies  Immune System Pathway Diagram
 Keap1-Nrf2 Pathway antibodies  Keap1-Nrf2 Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
 Ubiquitin Mediated Proteolysis Pathway antibodies  Ubiquitin Mediated Proteolysis Pathway Diagram
Diseases associated with anti-KEAP1 antibodyOrgans/Tissues associated with anti-KEAP1 antibody
 Disease Name  Pubmed Publications
 Inflammation Antibodies  >170 publications with KEAP1 and Inflammation
 Carcinoma Antibodies  >126 publications with KEAP1 and Carcinoma
 Liver Diseases Antibodies  >87 publications with KEAP1 and Liver Diseases
 Nervous System Diseases Antibodies  >73 publications with KEAP1 and Nervous System Diseases
 Lung Neoplasms Antibodies  >66 publications with KEAP1 and Lung Neoplasms
 Necrosis Antibodies  >50 publications with KEAP1 and Necrosis
 Kidney Diseases Antibodies  >48 publications with KEAP1 and Kidney Diseases
 Liver Neoplasms Antibodies  >44 publications with KEAP1 and Liver Neoplasms
 Carcinoma, Hepatocellular Antibodies  >41 publications with KEAP1 and Carcinoma, Hepatocellular
 Disease Progression Antibodies  >38 publications with KEAP1 and Disease Progression
 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >205 publications with KEAP1 and Liver
 Lung Antibodies  >146 publications with KEAP1 and Lung
 Blood Antibodies  >101 publications with KEAP1 and Blood
 Brain Antibodies  >83 publications with KEAP1 and Brain
 Kidney Antibodies  >77 publications with KEAP1 and Kidney
 Vascular Antibodies  >61 publications with KEAP1 and Vascular
 Heart Antibodies  >56 publications with KEAP1 and Heart
 Skin Antibodies  >52 publications with KEAP1 and Skin
 Muscle Antibodies  >46 publications with KEAP1 and Muscle
 Nerve Antibodies  >35 publications with KEAP1 and Nerve
Table BarTOPTable Bar
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions