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anti-KSR1 antibody :: Rabbit anti-Human, Mouse KSR1 Polyclonal Antibody

Scan QR to view Datasheet Catalog #    MBS9600686
Unit / Price
0.1 mL  /  $255 +1 FREE 8GB USB
0.2 mL  /  $295 +1 FREE 8GB USB
 
 Go to:   rightarrow  Product Names   rightarrow Product Info   rightarrow Accession #s   rightarrow Product Desc   rightarrow Diseases/Tissues/Pathways   rightarrow Applications   rightarrow References 
 Product Name   

KSR1, Polyclonal Antibody

★Popular Item★
 Also Known As   

Phospho-KSR1 (Ser392/406) Antibody

 Product Synonym Names    BKSR 1; BKSR1; dKsr; EK 31; EK31; hb; Kinase suppressor of Ras 1; Kinase suppressor of ras; KSR 1; KSR; KSR1; KSR1_HUMAN; Positive Ras signaling mediator family member (86.4 kD); Positive Ras signaling mediator family member; RSU 2; RSU2; SR 31; SR31; Suppressor of activated let 60 Ras SUR3; Suppressor of Ras1 31
 Product Gene Name   

anti-KSR1 antibody

[Similar Products]
 Research Use Only    For Research Use Only. Not for use in diagnostic procedures.
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 3D Structure    ModBase 3D Structure for Q8IVT5
 Clonality    Polyclonal
 Isotype    IgG
 Host    Rabbit
 Species Reactivity    Human, Mouse
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 Specificity    Phospho-KSR1 (Ser392/406) antibody detects endogenous levels of KSR1.
 Purity/Purification    From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
 Form/Format    Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
 Concentration    1mg/ml (lot specific)
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 Immunogen    A synthesized peptide derived from human KSR1 around the phosphorylation site of Ser392/406.
 Subcellular Location    Endoplasmic Reticulum; Plasma Membrane
 Conjugation    Unconjugated
 Epitope    Phospho Ser392, Phospho Ser406
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 Preparation and Storage    Store at -20 degree C. Stable for 12 months from date of receipt.
 Other Notes    Small volumes of anti-KSR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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Related Product Information for anti-KSR1 antibody

   Function: Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself.
Subunit Structure: Interacts with HSP90AA1/HSP90, YWHAE/14-3-3, CDC37, MAP2K1/MEK-1 and MAP2K2/MEK-2 (PubMed:10409742). Interacts with MARK3, PPP2R1A and PPP2CA. Also interacts with RAF and MAPK/ERK, in a Ras-dependent manner. The binding of 14-3-3 proteins to phosphorylated KSR prevents the membrane localization (By similarity). Interacts with isoform 1 of VRK2 (PubMed:20679487). Interacts with AKAP13 and BRAF (PubMed:21102438). Identified in a complex with AKAP13, MAP2K1 and BRAF (By similarity).
Post-translational Modifications: Phosphorylated on Ser-311 and, to a higher extent, on Ser-406 by MARK3. Dephosphorylated on Ser-406 by PPP2CA. In resting cells, phosphorylated KSR1 is cytoplasmic and in stimulated cells, dephosphorylated KSR1 is membrane-associated. Phosphorylated by PKA at Ser-888. Phosphorylation at Ser-888 is required for cAMP-dependent activation of MAPK1 and/or MAPK3 (By similarity).
Similarity: The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation (PubMed:10409742).The protein kinase domain is predicted to be catalytically inactive. The domain is sufficient for KSR1 and KSR1-mediated MAP2K1 and MAP2K2 membrane localization. The domain is required but not sufficient for MAP kinase-mediated inhibition of ELK1 phosphorylation.The N-terminal region mediates interaction with BRAF and with membranes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
 Applications Tested/Suitable for anti-KSR1 antibody   

Western Blot (WB), Immunohistochemistry (IHC)

 Application Notes for anti-KSR1 antibody    WB: 1:500-1:2000
IHC: 1:50-1:200
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 Immunohistochemistry (IHC) of anti-KSR1 antibody    MBS9600686 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
anti-KSR1 antibody Immunohistochemistry (IHC) (IHC) image
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 Western Blot (WB) of anti-KSR1 antibody    Western blot analysis of extracts of HEK293 cells, using Phospho-KSR1 (Ser392/406) Antibody.
anti-KSR1 antibody Western Blot (WB) (WB) image
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NCBI/Uniprot data below describe general gene information for KSR1. It may not necessarily be applicable to this product.
 NCBI GI #    75677325
 NCBI GeneID    8844
 NCBI Accession #    NP_055053.1 [Other Products]
 NCBI GenBank Nucleotide #    NM_014238.1 [Other Products]
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 UniProt Primary Accession #    Q8IVT5 [Other Products]
 UniProt Secondary Accession #    Q13476; F8WEA9; H7BYU0 [Other Products]
 UniProt Related Accession #    Q8IVT5 [Other Products]
 Molecular Weight    Observed: 115 kDa
Predicted: 103 kDa
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 NCBI Official Full Name    kinase suppressor of Ras 1
 NCBI Official Synonym Full Names    kinase suppressor of ras 1
 NCBI Official Symbol    KSR1 [Similar Products]
 NCBI Official Synonym Symbols   
KSR; RSU2
[Similar Products]
 NCBI Protein Information    kinase suppressor of Ras 1
 UniProt Protein Name    Kinase suppressor of Ras 1
 Protein Family    Kinase suppressor of Ras
 UniProt Gene Name    KSR1 [Similar Products]
 UniProt Synonym Gene Names    KSR [Similar Products]
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 UniProt Comments for KSR1    Scaffolding protein that is part of a multiprotein signaling complex. Promotes phosphorylation of Raf family members and activation of downstream MAP kinases. Promotes activation of MAPK1 and/or MAPK3, both in response to EGF and to cAMP. Does not have kinase activity by itself.
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 Research Articles on KSR1    1. findings demonstrate that KSR-MEK complexes allosterically activate BRAF through the action of N-terminal regulatory region and kinase domain contacts and challenge the accepted role of KSR as a scaffold for MEK recruitment to RAF
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 Precautions    All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
 Disclaimer    While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
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Products associated with anti-KSR1 antibodyPathways associated with anti-KSR1 antibody
 Reference Product  PubMed Publications
 MAPK1 antibody  >9 publications with KSR1 and MAPK1
 MAP2K1 antibody  >3 publications with KSR1 and MAP2K1
 MARK3 antibody  >2 publications with KSR1 and MARK3
 RAF1 antibody  >1 publications with KSR1 and RAF1
 NME1 antibody  >1 publications with KSR1 and NME1
 Products by Pathway  Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Ceramide Signaling Pathway antibodies  Ceramide Signaling Pathway Diagram
 ErbB1 Downstream Signaling Pathway antibodies  ErbB1 Downstream Signaling Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
 Tuberculosis Pathway antibodies  Tuberculosis Pathway Diagram
Diseases associated with anti-KSR1 antibodyOrgans/Tissues associated with anti-KSR1 antibody
 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >16 publications with KSR1 and Neoplasms
 Necrosis Antibodies  >5 publications with KSR1 and Necrosis
 Death Antibodies  >5 publications with KSR1 and Death
 Breast Neoplasms Antibodies  >4 publications with KSR1 and Breast Neoplasms
 Inflammation Antibodies  >3 publications with KSR1 and Inflammation
 Carcinoma, Squamous Cell Antibodies  >2 publications with KSR1 and Carcinoma, Squamous Cell
 Drug Toxicity Antibodies  >2 publications with KSR1 and Drug Toxicity
 Lung Neoplasms Antibodies  >2 publications with KSR1 and Lung Neoplasms
 Ovarian Neoplasms Antibodies  >2 publications with KSR1 and Ovarian Neoplasms
 Ovarian Diseases Antibodies  >2 publications with KSR1 and Ovarian Diseases
 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >6 publications with KSR1 and Blood
 Lung Antibodies  >5 publications with KSR1 and Lung
 Intestine Antibodies  >3 publications with KSR1 and Intestine
 Embryonic Tissue Antibodies  >3 publications with KSR1 and Embryonic Tissue
 Brain Antibodies  >3 publications with KSR1 and Brain
 Vascular Antibodies  >2 publications with KSR1 and Vascular
 Muscle Antibodies  >2 publications with KSR1 and Muscle
 Adipose Tissue Antibodies  >1 publications with KSR1 and Adipose Tissue
 Heart Antibodies  >1 publications with KSR1 and Heart
 Eye Antibodies  >1 publications with KSR1 and Eye
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